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Development Genes and Evolution

, Volume 223, Issue 5, pp 319–328 | Cite as

Identification and characterization of a twist ortholog in the polychaete annelid Platynereis dumerilii reveals mesodermal expression of Pdu-twist

  • Kathrin Pfeifer
  • Christoph Schaub
  • Georg Wolfstetter
  • Adriaan DorresteijnEmail author
Original Article

Abstract

The basic helix-loop-helix transcription factor twist plays a key role during mesoderm development in Bilateria. In this study, we identified a twist ortholog in the polychaete annelid Platynereis dumerilii and analyze its expression during larval development, postlarval growth up to the adult stage, and caudal regeneration after amputation of posterior segments. At late larval stages, Pdu-twist is expressed in the mesodermal anlagen and in developing muscles. During adulthood and caudal regeneration, Pdu-twist is expressed in the posterior growth zone, in mesodermal cells within the newly forming segments and budding parapodia. Our results indicate that Pdu-twist is involved in mesoderm formation during larval development, posterior growth, and caudal regeneration.

Keywords

Regeneration Muscle Transcription factor Posterior growth Segmentation 

Notes

Acknowledgments

The authors would like to thank Brigitte Fronk, Renate Plaß, and Susanne Vasoldt-Kröckel for maintenance of the Platynereis dumerilii culture.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Kathrin Pfeifer
    • 1
  • Christoph Schaub
    • 2
  • Georg Wolfstetter
    • 3
  • Adriaan Dorresteijn
    • 1
    Email author
  1. 1.Institut für Allgemeine und Spezielle Zoologie, Allgemeine Zoologie und EntwicklungsbiologieJustus-Liebig-Universität GießenGiessenGermany
  2. 2.Institut für Biologie, Abteilung EntwicklungsbiologieFriedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany
  3. 3.Department of Molecular BiologyUmea University (Sweden)UmeaSweden

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