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RNA interference can be used to disrupt gene function in tardigrades

Abstract

How morphological diversity arises is a key question in evolutionary developmental biology. As a long-term approach to address this question, we are developing the water bear Hypsibius dujardini (Phylum Tardigrada) as a model system. We expect that using a close relative of two well-studied models, Drosophila (Phylum Arthropoda) and Caenorhabditis elegans (Phylum Nematoda), will facilitate identifying genetic pathways relevant to understanding the evolution of development. Tardigrades are also valuable research subjects for investigating how organisms and biological materials can survive extreme conditions. Methods to disrupt gene activity are essential to each of these efforts, but no such method yet exists for the Phylum Tardigrada. We developed a protocol to disrupt tardigrade gene functions by double-stranded RNA-mediated RNA interference (RNAi). We showed that targeting tardigrade homologs of essential developmental genes by RNAi produced embryonic lethality, whereas targeting green fluorescent protein did not. Disruption of gene functions appears to be relatively specific by two criteria: targeting distinct genes resulted in distinct phenotypes that were consistent with predicted gene functions and by RT-PCR, RNAi reduced the level of a target mRNA and not a control mRNA. These studies represent the first evidence that gene functions can be disrupted by RNAi in the phylum Tardigrada. Our results form a platform for dissecting tardigrade gene functions for understanding the evolution of developmental mechanisms and survival in extreme environments.

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Acknowledgments

We thank Bob McNuff for the continuing help with tardigrade cultures, Victoria Madden for helping with electron microscopy, and members of the Goldstein lab for the critical reading of this manuscript. J.R.T. was supported by the National Institutes of Health, Minority Opportunities in Research division of the National Institute of General Medical Sciences (NIGMS) grant K12GM000678 and by the NIH-funded UNC Developmental Biology Training Grant (T32HD046369-03). S.M. was supported by ARRA funding from the Minority Opportunities in Research (MORE) division of the National Institute of General Medical Sciences (NIGMS) supplementing the IRACDA/SPIRE grant K12GM000678. This work was supported by NSF grants IOS 0235658 and IOS 0652007 to B.G.

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Correspondence to Jennifer R. Tenlen.

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Communicated by: Dahua Chen

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Tenlen, J.R., McCaskill, S. & Goldstein, B. RNA interference can be used to disrupt gene function in tardigrades. Dev Genes Evol 223, 171–181 (2013). https://doi.org/10.1007/s00427-012-0432-6

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Keywords

  • Hypsibius dujardini
  • Tardigrade
  • RNA interference
  • Evo-devo
  • Extreme environments