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The embryonic development of the malacostracan crustacean Porcellio scaber (Isopoda, Oniscidea)

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Abstract

To examine the evolution of development and put it into a phylogenetic context, it is important to have, in addition to a model organism like Drosophila, more insights into the huge diversity of arthropod morphologies. In recent years, the malacostracan crustacean Porcellio scaber Latreille, 1804 has become a popular animal for studies in evolutionary and developmental biology, but a detailed and complete description of its embryonic development is still lacking. Therefore, the embryonic development of the woodlouse P. scaber is described in a series of discrete stages easily identified by examination of living animals and the widely used technique of nuclei staining on fixed specimens. It starts with the first cleavage of the zygote and ends with a hatched manca that eventually leaves the mother’s brood pouch. Classical methods like normal light microscopy, scanning electron microscopy and fluorescence microscopy are used, in addition to confocal LCM and computer-aided 3D reconstruction in order to visualise important processes during ontogeny. The purpose of these studies is to offer an easy way to define the different degrees of development for future comparative analyses of embryonic development amongst crustaceans in particular, as well as between different arthropod groups. In addition, several aspects of Porcellio embryonic development, such as the mouth formation, limb differentiations and modifications or the formation of the digestive tract, make this species particularly interesting for future studies in evolutionary and developmental biology.

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Acknowledgements

I thank R. Mbacke for the help with collecting specimens and G. Drescher (Natural History Museum, Berlin) for the support in using the SEM. I also thank Kristen Panfilio and the two reviewers for the helpful advice and Greg Edgecombe for improving the English.

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Correspondence to Carsten Wolff.

Additional information

Communicated by S. Roth

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Supplementary file. Mpg movie: Gut_peristaltic_stage15: The time lapse movie (normal light microscope) shows the peristaltic contractions of the midgut anlagen in a living Porcellio embryo (lateral view, anterior is left). Note that only the posterior part of the midgut is moving. While the midgut tubes elongate more and more during development, the contractions are more frequent (approximately two times per minute). (MPG 498 kb)

Supplementary file. Mpg movie: Gut_peristaltic_stage15: The time lapse movie (normal light microscope) shows the peristaltic contractions of the midgut anlagen in a living Porcellio embryo (lateral view, anterior is left). Note that only the posterior part of the midgut is moving. While the midgut tubes elongate more and more during development, the contractions are more frequent (approximately two times per minute). (MPG 498 kb)

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Wolff, C. The embryonic development of the malacostracan crustacean Porcellio scaber (Isopoda, Oniscidea). Dev Genes Evol 219, 545–564 (2009) doi:10.1007/s00427-010-0316-6

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Keywords

  • Arthropods
  • Isopoda
  • Embryogenesis
  • Evolution
  • Direct development
  • Crustacea