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Development Genes and Evolution

, Volume 218, Issue 10, pp 525–539 | Cite as

Embryonic development and metamorphosis of the scyphozoan Aurelia

  • David Yuan
  • Nagayasu Nakanishi
  • David K. Jacobs
  • Volker Hartenstein
Original Article

Abstract

We investigated the development of Aurelia (Cnidaria, Scyphozoa) during embryogenesis and metamorphosis into a polyp, using antibody markers combined with confocal and transmission electron microscopy. Early embryos form actively proliferating coeloblastulae. Invagination is observed during gastrulation. In the planula, (1) the ectoderm is pseudostratified with densely packed nuclei arranged in a superficial and a deep stratum, (2) the aboral pole consists of elongated ectodermal cells with basally located nuclei forming an apical organ, which is previously only known from anthozoan planulae, (3) endodermal cells are large and highly vacuolated, and (4) FMRFamide-immunoreactive nerve cells are found exclusively in the ectoderm of the aboral region. During metamorphosis into a polyp, cells in the planula endoderm, but not in the ectoderm, become strongly caspase 3 immunoreactive, suggesting that the planula endoderm, in part or in its entirety, undergoes apoptosis during metamorphosis. The polyp endoderm seems to be derived from the planula ectoderm in Aurelia, implicating the occurrence of “secondary” gastrulation during early metamorphosis.

Keywords

Cnidaria Scyphozoa Embryogenesis Planula Polyp 

Notes

Acknowledgements

We thank Mike Schaadt and Kiersten Darrow of the Cabrillo Marine aquarium, San Pedro, CA, USA for providing us with the Aurelia material. This work was supported by the UCLA Edwin W. Pauley fellowship (to N.N.) and the NASA Astrobiology Institute. We also thank anonymous reviewers for helpful comments on the manuscript.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • David Yuan
    • 1
  • Nagayasu Nakanishi
    • 1
  • David K. Jacobs
    • 1
  • Volker Hartenstein
    • 2
    • 3
  1. 1.Department of Ecology and Evolutionary BiologyUCLALos AngelesUSA
  2. 2.Department of Molecular, Cellular, and Developmental BiologyUCLALos AngelesUSA
  3. 3.Department of Molecular, Cell, and Developmental BiologyUC Los AngelesLos AngelesUSA

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