Development Genes and Evolution

, Volume 218, Issue 5, pp 237–251

Expression patterns of engrailed and dpp in the gastropod Lymnaea stagnalis

  • Minoru Iijima
  • Takeshi Takeuchi
  • Isao Sarashina
  • Kazuyoshi Endo
Original Article


We isolated the full-length cDNAs of engrailed and dpp-BMP2/4 orthologues from the pond snail Lymnaea stagnalis and examined their expression patterns during development by the whole mount in situ hybridization. At the gastrula and trochophore stages, engrailed is expressed in the peripheral ectoderm of the presumptive and invaginating shell gland, corroborating its role in the shell formation that is widely conserved among molluscs. At the same stages, dpp-BMP2/4 is expressed in the right-hand side ectoderm of the shell gland and in the invaginating stomodaeum. Unlike in the gastropod Patella vulgata, our results suggested that dpp-BMP2/4 has a role in the shell formation, rather than in the regional specification and that it could be involved in the specification pathway of the left–right asymmetry of the developing shell in L. stagnalis.


Gastropod mollusk Lymnaea stagnalis Shell formation Engrailed dpp-BMP2/4 


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Minoru Iijima
    • 1
  • Takeshi Takeuchi
    • 1
  • Isao Sarashina
    • 1
  • Kazuyoshi Endo
    • 1
  1. 1.Department of Earth Evolution Sciences, Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan

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