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Pumilio genes from the Platyhelminthes

  • Uriel Koziol
  • Monica Marín
  • Estela Castillo
Sequence Corner

Abstract

Pumilio proteins are proposed to have a conserved primordial function in the maintenance of proliferation in stem cells through post-transcriptional regulation. In this work, a search for pumilio homology domain (PUM-HD) sequences of pumilio genes from several Platyhelminthes species was performed, including representatives form Cestoda, Trematoda and Tricladida. Only one PUM-HD sequence was found in each triclad species; however, two PUM-HD homologues were found in all the parasitic species. These sequences formed two clearly separated clades: PlatyPum1, with sequences from all species, and PlatyPum2, composed exclusively of neodermatan sequences. Therefore, at least one duplication of the pumilio gene must have occurred before the divergence of cestodes and trematodes. Further duplications of PUM-HD were found in Fasciola hepatica, but these consist of retropseudogenes. This is the first comparative analysis of PUM-HD sequences in the Platyhelminthes and, more generally, in any lophotrochozoan phylum.

Keywords

Pumilio Platyhelminthes Mesocestoides Echinococcus Fasciola 

Notes

Acknowledgments

The authors thank Laura Dominguez and Jenny Saldaña for providing infected mice, Lucia Canclini for her help with M. corti cultures, Jose Tort and Gabriel Rinaldi for biological samples and genomic DNA of F. hepatica, and Rodrigo Ponce de León for biological samples of planarians.

Supplementary material

427_2007_200_MOESM1_ESM.tif (142 kb)
Supplementary Figure 1 Amplification of PUM-HD in different developmental stages from M. corti. RT-PCR was performed with degenerate primers for PUM-HD. 3d 3 day culture tetrathyridia; 11d 11 day culture elongated tetrathyridia; S segmented animals. Arrows point to the McPum1 (1) and McPum2 (2) amplification products. No amplification products were obtained in a negative control without cDNA (not shown). M Molecular weight marker; molecular weights are indicated on the left (TIF 145 KB)
427_2007_200_MOESM2_ESM.doc (22 kb)
Supplementary Table 1 Pumilio sequences from the Platyhelminthes (GenBank) (DOC 22 KB)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Sección Bioquímica, Facultad de CienciasUniversidad de la RepúblicaMontevideoUruguay

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