Development Genes and Evolution

, Volume 217, Issue 6, pp 413–420

Both Hoxc13 orthologs are functionally important for zebrafish tail fin regeneration

  • Ryan Thummel
  • Mila Ju
  • Michael P. SarrasJr.
  • Alan R. Godwin
Original Article


Hox genes are re-expressed during regeneration in many species. Given their important role in body plan development, it has been assumed, but not directly shown, that they play a functional role in regeneration. In this paper we show that morpholino-mediated knockdown of either Hoxc13a or Hoxc13b during the process of zebrafish tail fin regeneration results in a significant reduction of regenerative outgrowth. Furthermore, cellular proliferation within the blastema is directly affected in both knockdowns. Hence, similar to the demonstration of unique functions of multiple Hox genes during limb formation, both Hoxc13 orthologs have distinct functions in regeneration.


Hox Regeneration Morpholino Electroporation Zebrafish 


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Ryan Thummel
    • 1
    • 2
  • Mila Ju
    • 3
  • Michael P. SarrasJr.
    • 3
    • 4
  • Alan R. Godwin
    • 1
  1. 1.Department of Molecular and Integrative PhysiologyUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Center for Zebrafish Research and Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  3. 3.Department of Anatomy and Cell BiologyUniversity of Kansas Medical CenterKansas CityUSA
  4. 4.Department of Cell Biology and AnatomyRosalind Franklin University of Medicine and ScienceNorth ChicagoUSA

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