Development Genes and Evolution

, Volume 217, Issue 1, pp 63–72

Rel homology domain-containing transcription factors in the cnidarian Nematostella vectensis

  • James C. Sullivan
  • Demetrios Kalaitzidis
  • Thomas D. Gilmore
  • John R. Finnerty
Sequence Corner

Abstract

The Rel/NF-κB and NFAT families of transcription factors are related through an N-terminal DNA-binding domain called the Rel Homology domain (RHD). Neither the RHD nor the NF-κB pathway has been identified in a basal (i.e., nonbilaterian) animal phylum. Using genomic and cDNA databases, we have identified two RHD domain-containing proteins from the cnidarian Nematostella vectensis: an NF-κB-like protein (Nv-NF-κB) and an NFAT-like protein (Nv-NFAT). The gene structure and RHD predicted amino acid sequence of Nv-nfkb are similar to those of the vertebrate NF-κB p50/p52 proteins, whereas the sequence of Nv-NFAT allows only ambiguous assignment to the NFAT family. Nv-NF-κB lacks the C-terminal IκB-like sequences present in all other NF-κB proteins. There are, however, two IκB-like genes in Nematostella encoded by loci distinct from Nv-nfkb. The separate nfkb and ikb genes of Nematostella may reflect the ancestral metazoan condition, suggesting that a gene fusion event created the nfkb genes in Drosophila and vertebrates. Nematostella also has genes that encode upstream and downstream components of the vertebrate NF-κB signaling pathway. Upstream components include Toll- and tumor necrosis-like receptors and ligands, adaptor proteins (Trafs, Myd88), caspases, and a TBK-like kinase. Downstream components include the NF-κB coactivator protein Bcl-3 and several NF-κB target genes. These results demonstrate that RHD-containing transcription factors and associated pathways are evolutionarily more ancient than previously known. Moreover, they suggest models for the evolutionary diversification of the insect and vertebrate Rel/NF-κB/IκB and NFAT gene families and suggest that cnidarians possess an NF-κB-regulated developmental or stress response pathway.

Keywords

NF-kappaB NFAT IkappaB Rel homology domain Evolution 

Supplementary material

427_2006_111_MOESM1_ESM.doc (32 kb)
Table S1Nomenclature of RHD and IκB-like proteins (DOC 32 kb)
427_2006_111_MOESM2_ESM.doc (48 kb)
Table S2Genbank accession numbers and taxon ID’s of taxa used in phylogenetic analyses (DOC 48 kb)
427_2006_111_Fig1_ESM.jpg (454 kb)
Fig. S1

Alignment used in phylogenetic analyses of the IκB family represented in Fig. 4 (JPEG 465 kb)

427_2006_111_Fig1_ESM.tif (8.7 mb)
High resolution image file (TIFF 9,157 kb)

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • James C. Sullivan
    • 1
  • Demetrios Kalaitzidis
    • 2
  • Thomas D. Gilmore
    • 1
  • John R. Finnerty
    • 1
  1. 1.Department of BiologyBoston UniversityBostonUSA
  2. 2.Hematology–Oncology/Cancer Biology Program, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA

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