Development Genes and Evolution

, Volume 216, Issue 9, pp 543–550 | Cite as

Drosophila RhoGAP68F is a putative GTPase activating protein for RhoA participating in gastrulation

  • Justina Sanny
  • Vincent Chui
  • Caillin Langmann
  • Carla Pereira
  • Baharak Zahedi
  • Nicholas Harden
Short Communication
First Online:
Received:
Accepted:

Abstract

The Rho family small GTPases Rho, Rac, and Cdc42 regulate cell shape and motility through the actin cytoskeleton. These proteins cycle between a GTP-bound “on” state and a GDP-bound “off” state and are negatively regulated by GTPase-activating proteins (GAPs), which accelerate the small GTPase’s intrinsic hydrolysis of bound GTP to GDP. Drosophila RhoGAP68F is similar to the mammalian protein p50RhoGAP/Cdc42GAP, which exhibits strong GAP activity toward Cdc42. We find that, despite the strong similarities between RhoGAP68F and p50RhoGAP/Cdc42GAP, RhoGAP68F is most effective as a GAP for RhoA. These in vitro data are supported by the in vivo analysis of mutants in RhoGAP68F. We demonstrate through the characterization of two alleles of the RhoGAP68F gene that RhoGAP68F participates in gastrulation of the embryo, a morphogenetic event driven by cell constriction that involves RhoA signaling. We propose that RhoGAP68F functions as a regulator of RhoA signaling during gastrulation.

Keywords

RhoGAP68F RhoA GTPase activating protein Drosophila Gastrulation 
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Notes

Acknowledgements

We thank Y. Y. Ng, R. Matsumora, and V. Santiago for assistance; Esther Verheyen for comments on the manuscript; H. Bellen, C. Magie, S. Parkhurst, and the Bloomington Stock Center for fly stocks; and S. D. Menon and D. Kiehart for antibodies. This work was supported by a grant to N. H. from the Natural Sciences and Engineering Research Council of Canada.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Justina Sanny
    • 1
  • Vincent Chui
    • 1
  • Caillin Langmann
    • 1
  • Carla Pereira
    • 1
  • Baharak Zahedi
    • 1
  • Nicholas Harden
    • 1
  1. 1.Department of Molecular Biology and BiochemistrySimon Fraser UniversityBurnabyCanada

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