Development Genes and Evolution

, Volume 216, Issue 6, pp 291–299 | Cite as

Identification of alternatively spliced dab1 isoforms in zebrafish

  • Arianna Costagli
  • Barbara Felice
  • Alessandro Guffanti
  • Stephen W. Wilson
  • Marina Mione
Original Article


We have investigated the genomic organization, the occurrence of alternative splicing and the differential expression of the zebrafish disabled1 (dab1) gene. Dab1 is a key effector of the Reelin pathway, which regulates neuronal migration during brain development in vertebrates. The coding region of the zebrafish dab1 gene spans over 600 kb of genomic DNA and is composed of 15 exons. Alternative splicing in a region enriched for tyrosine residues generates at least three different isoforms. These isoforms are developmentally regulated and show differential tissue expression. Comparison with mouse and human data shows an overall conservation of the genomic organization with different alternative splicing events generating species-specific isoforms. Because these alternative splicing events give rise to isoforms with different numbers of phosphorylateable tyrosines, we speculate that alternative splicing of the dab1 gene in zebrafish and in other vertebrates regulates the nature of the cellular response to the Reelin signal.


Disabled1 Neuronal migration Transcriptional variants Reelin Tyrosine phosphorylation 



We would like to thank the UCL Zebrafish Facility and the UCL Zebrafish Group for their help throughout this work. We are grateful to Dr. Mario Caccamo, Wellcome Trust Sanger Institute, for helping us with the genomic data. The project was supported by Wellcome Trust grants to Steve Wilson.

Supplementary material

427_2005_52_MOESM1_ESM.pdf (526 kb)
Fig. S1(PDF 538 kb)
427_2005_52_MOESM2_ESM.pdf (73 kb)
Fig. S2(PDF 75 kb)
427_2005_52_MOESM3_ESM.pdf (36 kb)
Table S1(PDF 37 kb)


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Arianna Costagli
    • 1
  • Barbara Felice
    • 2
  • Alessandro Guffanti
    • 2
  • Stephen W. Wilson
    • 1
  • Marina Mione
    • 1
    • 2
  1. 1.Department of Anatomy and Developmental BiologyUniversity College LondonLondonUK
  2. 2.IfomFirc Institute of Molecular OncologyMilanItaly

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