Development Genes and Evolution

, Volume 214, Issue 4, pp 159–169 | Cite as

Isolation and evaluation of dextral-specific and dextral-enriched cDNA clones as candidates for the handedness-determining gene in a freshwater gastropod, Lymnaea stagnalis

  • Yoshito Harada
  • Yuji Hosoiri
  • Reiko KurodaEmail author
Original Article


The handedness of gastropods is genetically determined. The freshwater gastropod Lymnaea stagnalis is a normally dextral species, but contains minor sinistral populations. The gene responsible for handedness determination in this species is predicted to function maternally and specifically in the dextral-ovipositing snail. In this study, we used differential screening and cDNA subtraction to isolate eight “dextral genes” that are specific to, or enriched in, the dextral-ovipositing strains of L. stagnalis. These genes were promising candidates for the handedness-determining gene. In order to determine whether the true handedness-determining gene was among them, we tested for genetic correlations between the level of expression of each dextral gene and the handedness phenotype, i.e., the chirality of the next generation offspring, by using a collection of backcross F2 progeny of F1 offspring from crosses between dextral and sinistral strains. Although the present study could not identify the handedness-determining molecules, this approach appears to be promising for the isolation of such developmentally important genes.


Handedness Gastropod Differential screening cDNA subtraction Genetic evaluation 



We are grateful to Prof. Gary Freeman and Judith W. Lundelius (University of Texas) for their advice on the construction and maintenance of the laboratory snail stocks, and to Prof. Takahiro Asami (Shinshu University) for his kind assistance in obtaining the snails. We thank Dr. Miho Shimizu in our laboratory for her help with construction of the libraries. We also wish to thank other past and present members of our laboratory, especially Midori Kodama, Yuki Kadoishi, Keita Miyoshi, and Kota Yoshimatsu, for their efforts in maintaining the snail stocks. The sequences reported in this paper will appear in the DDBJ database with the respective accession numbers (DSC-1: AB159144, DSC-2: AB159145, DIC-1: AB159146, DIC-2: AB159147, DIC-3: AB159148, DEC-1: AB159149, DEC-2: AB159150, DEC-3: AB159151, RIC-1: AB159152, and YGSC-1: AB159153).


  1. Asami T (1993) Genetic variation and evolution of coiling chirality in snails. Forma 8:263–276Google Scholar
  2. Bottke W, Burschyk M, Vollmer J (1988) On the origin of the yolk protein ferritin in snails. Roux’s Arch Dev Biol 197:377–382Google Scholar
  3. Crampton HE (1894) Reversal of cleavage in a sinistral gastropod. Ann NY Acad Sci 8:167–170Google Scholar
  4. Freeman G (1983) The role of egg organization in the generation of cleavage patterns. In: Jeffery WR, Raff RA (eds) Time, space, and pattern in embryonic development. Wiley-Liss, New York, pp 171–196Google Scholar
  5. Freeman G, Lundelius JW (1982) The developmental genetics of dextrality and sinistrality in the gastropod Lymnaea peregra. Wilhelm Roux Arch Entwicklungsmech Org 191:69–83Google Scholar
  6. Hosoiri Y, Harada Y, Kuroda R (2003) Construction of a backcross progeny collection of dextral and sinistral individuals of a freshwater gastropod, Lymnaea stagnalis. Dev Genes Evol 213:193–198PubMedGoogle Scholar
  7. Meshcheryakov VN, Beloussov LV (1975) Asymmetrical rotations of blastomeres in early cleavage of gastropoda. Wilhelm Roux Arch Entwicklungsmech Org 177:193–203Google Scholar
  8. Satou Y, Kusakabe T, Araki I, Satoh N (1995) Timing of initiation of muscle-specific gene expression in the ascidian embryo precedes that of developmental fate restriction in lineage cells. Dev Growth Differ 37:319–327CrossRefGoogle Scholar
  9. Sturtevant AH (1923) Inheritance of direction of coiling in Limnaea. Science 58:269–270Google Scholar
  10. Supp DM, Witte DP, Potter SS, Brueckner M (1997) Mutation of an axonemal dynein affects left-right asymmetry in inversus viscerum mice. Nature 389:963–966PubMedGoogle Scholar
  11. Thitamadee S, Tuchihara K, Hashimoto T (2002) Microtubule basis for left-handed helical growth in Arabidopsis. Nature 417:193–196CrossRefPubMedGoogle Scholar
  12. Xu B, Janson JC, Sellos D (2001) Cloning and sequencing of a molluscan endo-β-1,4-glucanase gene from the blue mussel, Mytilus edulis. Eur J Biochem 268:3718–3727PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Kuroda Chiromorphology Project, ERATOJapan Science and Technology Corporation (JST)TokyoJapan
  2. 2.Department of Life Sciences, Graduate School of Arts and SciencesUniversity of TokyoTokyoJapan
  3. 3.Sugashima Marine Biological Laboratory, Graduate School of ScienceNagoya UniversityMieJapan

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