Development Genes and Evolution

, Volume 213, Issue 7, pp 363–367 | Cite as

Complementary expression of AP-2 and AP-2rep in ectodermal derivatives of Xenopus embryos

Expression Note

Abstract

In an attempt to define the pattern of developmental expression of AP-2rep and AP-2 in Xenopus embryos, we cloned a Xenopus AP-2rep cDNA. The AP-2rep message was localized in the organizer region at the gastrula stage whereas AP-2 was expressed ventro-laterally in the animal hemisphere. Later, AP-2rep was expressed in the entire neural tissue at the neurula stage while AP-2 was predominantly expressed in the cranial neural crest areas. The endogenous expression of AP-2 in the neural crest area was diminished by ectopic injection of AP-2rep RNA, suggesting a role for AP-2rep in the differentiation of neural tissues by restricting the expression of AP-2 in the Xenopus embryo.

Keywords

Activator protein-2 KLF-12 Telencephalon Neural crest Repressor 

References

  1. Bieker JJ (2001) Krüppel-like factors: three fingers in many pies. J Biol Chem 276:34355–34358CrossRefPubMedGoogle Scholar
  2. Bourguignon C, Li J, Papalopulu, N (1998) XBF-1, a winged helix transcription factor with dual activity, has a role in positioning neurogenesis in Xenopus competent ectoderm. Development 125:4889–4900PubMedGoogle Scholar
  3. Chazaud C, Oulad-Abdelghani M, Bouillet P, Decimo D, Chambon P, Dolle P (1996) AP-2.2, a novel gene related to AP-2, is expressed in the forebrain, limbs and face during mouse embryogenesis. Mech Dev 54:83–94CrossRefPubMedGoogle Scholar
  4. Hemmati-Brivanlou A, de la Torre JR, Holt C, Harland RM (1991) Cephalic expression and molecular characterization of Xenopus En-2. Development 111:715–724PubMedGoogle Scholar
  5. Imhof A, Schuierer M, Werner O, Moser M, Roth C, Bauer R, Buettner R (1999) Transcriptional regulation of the AP-2α promoter by BTEB-1 and AP-2rep, a novel wt-1/erg-related zinc finger repressor. Mol Cell Biol 19:194–204PubMedGoogle Scholar
  6. Kawahara A, Dawid IB (2000) Expression of the Krüppel-like zinc finger gene bilkf during zebrafish development. Mech Dev 97:173–176CrossRefPubMedGoogle Scholar
  7. Kawahara A, Dawid IB (2001) Critical role of biklf in erythroid cell differentiation in zebrafish. Current Biol 17:1353–1357CrossRefGoogle Scholar
  8. Kikkawa M, Yamazaki M, Izutsu Y Maeno M (2001) Two-step induction of primitive erythrocytes in Xenopus laevis embryos: signals from the vegetal endoderm and the overlying ectoderm. Int J Dev Biol 45:387–396PubMedGoogle Scholar
  9. Luo T, Matsuo-Takasaki M, Thomas ML, Weeks DL, Sargent TD (2002) Transcription factor AP-2 is an essential and direct regulator of epidermal development in Xenopus. Dev Biol 245:136–144CrossRefPubMedGoogle Scholar
  10. Luo T, Leedagger YH, Saint-Jeannet JP, Sargent TD (2003) Induction of neural crest in Xenopus by transcription factor AP2α. Proc Natl Acad Sci USA 100:532–537CrossRefPubMedGoogle Scholar
  11. Mitchell PJ, Wang C, Tjian R (1987) Positive and negative regulation of transcription in vitro: Enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 50:847–861PubMedGoogle Scholar
  12. Monge I, Mitchell PJ (1998) DAP-2, the Drosophila homolog of transcription factor AP-2. Mech Dev 76:191–195CrossRefPubMedGoogle Scholar
  13. Moser M, Imhof A, Pscherer A, Bauer R, Amselgruber W, Sinowatz F, Hofstadter F, Schule R, Buettner R (1995) Cloning and characterization of a second AP-2 transcription factor: AP-2 β. Development 121:2779–2788PubMedGoogle Scholar
  14. Nieuwkoop PD, Faber J (1967) Normal table of Xenopus laevis (Daudin). North-Holland, AmsterdamGoogle Scholar
  15. Schorle H, Meier P, Buchrt M, Jaenisch R, Mitchell PJ (1996) Transcription factor AP-2 essential for cranial closure and craniofacial development. Nature 381:235–238Google Scholar
  16. Schuierer M, Hilger-Eversheim K, Dobner T, Bosserhoff AK, Moser M, Turner J, Crossley M, Buettner R (2001) Induction of AP-2α expression by adenoviral infection involves inactivation of the AP-2rep transcriptional corepressor CtBP1. J Biol Chem 276:27944–27949CrossRefPubMedGoogle Scholar
  17. Shain DH, Zuber MX (1996) Sodium dodecyl sulfate (SDS)- based whole-mount in situ hybridization of Xenopus embryo. J Biochem Biophys Meth 31:185–188PubMedGoogle Scholar
  18. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedGoogle Scholar
  19. West-Mays JA, Clyle BM, Piatigorsky J, Papagiotas S, Libby D (2002) Ectopic expression of AP-2α transcription factor in the lens disrupts fiber cell differentiation. Dev Biol 245:13–27CrossRefPubMedGoogle Scholar
  20. Zhang J, Hagopian-Donaldson S, Serbedzija G, Elsemore J, Plehn-Dujowich D, McMahon AP, Flavell RA, Williams T (1996) Neural tube, skeletal and body wall defects in mice lacking transcription factor AP-2. Nature 381:238–241Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Graduate School of Science and TechnologyNiigata UniversityNiigata 950-2181Japan
  2. 2.Department of Biology, Faculty of ScienceNiigata UniversityNiigata 950-2181Japan

Personalised recommendations