Advertisement

Development Genes and Evolution

, Volume 212, Issue 2, pp 99–103 | Cite as

Induction of gametogenesis in the basal cnidarian Nematostella vectensis (Anthozoa)

  • Jens H. Fritzenwanker
  • Ulrich Technau
Technical Note

Abstract.

A protocol was established to reproducibly induce spawning in the basal cnidarian Nematostella vectensis (Anthozoa). We found that a combination of feeding regime, dark-light cycle and temperature shift synergistically induced gametogenesis in adult polyps. Females lay between 100–600 eggs. This procedure led reproducibly to the production of thousands of eggs over the course of more than 1 year in weekly cycles. Gametes are released in a time window of about 2 h resulting in predictable and fairly synchronized development. We also present a method for in vitro fertilization allowing manipulation of early embryos. These methods as well as the simple culture conditions could provide important prerequisites for the use of Nematostella as a model system for the development of a basal Metazoa.

Cnidaria Nematostella vectensis Gametogenesis Gastrulation Planula 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Jens H. Fritzenwanker
    • 1
  • Ulrich Technau
    • 1
  1. 1.Molecular Cell Biology, Zoological Institute, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany

Personalised recommendations