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Development Genes and Evolution

, Volume 211, Issue 10, pp 506–508 | Cite as

The isolation of a Distal-less gene fragment from two molluscs

  • Shannon E. Lee
  • Ruth D. Gates
  • David K. Jacobs
Sequence Corner

Abstract.

The transcription factor, Distal-less, and its orthologues (e.g. DLL/Dlx) are best known for their roles in proximo-distal and nervous system patterning as well as for their use in inferring the evolution of limbs and other proximo-distal structures. However, evolutionary applications to non-model taxa have primarily employed a polyclonal antibody, and sequence data are generally lacking. We have designed a degenerate PCR primer set specific to the recovery of a Distal-less gene fragment, and present sequence data for two bivalve molluscs, Crassostrea gigas and Nutricola sp. These sequences represent the first Distal-less homologs isolated from lophotrochozoan taxa.

Distal-less Crassostrea gigas Nutricola sp. 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Shannon E. Lee
    • 1
  • Ruth D. Gates
    • 1
  • David K. Jacobs
    • 1
  1. 1.Department of Organismic Biology, Ecology, and Evolution, University of California, Los Angeles, 621 Charles E. Young Drive South, Los Angeles, CA 90095–1606, USA

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