Planta

, Volume 206, Issue 2, pp 225–233

Functional analysis and cell-specific expression of a phosphate transporter from tomato

  • Pierre Daram
  • Silvia Brunner
  • Bengt L. Persson
  • Nikolaus Amrhein
  • Marcel Bucher
Article

DOI: 10.1007/s004250050394

Cite this article as:
Daram, P., Brunner, S., Persson, B. et al. Planta (1998) 206: 225. doi:10.1007/s004250050394

Abstract.

For a better understanding of the molecular and biochemical processes involved in orthophosphate (Pi) uptake at the root/soil interface, we cloned a Pi-transporter cDNA (LePT1) from a root air-specific cDNA library of tomato (Lycopersicon esculentum Mill.). The corresponding protein belongs to the growing family of ion transporters with twelve putative transmembrane domains. It is highly homologous to recently isolated Pi transporters from higher plants, yeast and fungi. When expressed in a Pi-uptake-deficient yeast mutant, the L. esculentum phosphate transporter 1 (LePT1) protein exhibits an apparent Km of 31 μM. The transporter is still active at submicromolar Pi concentrations and mediates highest Pi uptake at pH 5. The activity of LePT1 is dependent on the electrochemical membrane potential mediated by the yeast P-type H+-ATPase. Transcript levels of LePT1 in tomato seedlings are detectable in all vegetative organs under Pi-sufficient conditions, with highest concentrations in root hairs. In␣situ hybridization studies demonstrate cell-specific expression of LePT1 in the tomato root. The LePT1 mRNA is detectable in peripheral cell layers such as rhizodermal and root cap cells. Under Pi-deprivation conditions, mRNA levels are also detectable in young stelar tissue. This work presents molecular and biochemical evidence for distinct root cells playing an important role in Pi acquisition at the root/soil interface.

Key words:Lycopersicon (Pi uptake) Phosphate transporter cDNA Rhizodermis Root cap Root hair Yeast complementation system 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Pierre Daram
    • 1
  • Silvia Brunner
    • 1
  • Bengt L. Persson
    • 2
  • Nikolaus Amrhein
    • 1
  • Marcel Bucher
    • 1
  1. 1.Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Experimental Station Eschikon 33, CH-8315 Lindau, SwitzerlandCH
  2. 2.Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, University of Stockholm, S-106 91 Stockholm, Sweden and Department of Engineering and Natural Sciences, Växjö University, S-351 95 Växjö, SwedenSE

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