Planta

, Volume 205, Issue 1, pp 132–139

The kinetics of sucrose concentration in the phloem of individual vascular bundles of the Ricinus communis seedling measured by nuclear magnetic resonance microimaging

  • Jutta Verscht
  • Bernhard Kalusche
  • Jutta Köhler
  • Walter Köckenberger
  • Alexander Metzler
  • Axel Haase
  • Ewald Komor
Article

Abstract.

The sucrose concentration was measured at 70-min intervals in the phloem of individual bundles of the hypocotyl of Ricinus seedlings by 1H nuclear magnetic resonance (NMR) spectroscopic imaging. The sucrose concentration stayed fairly constant in all bundles for more than 7 h if the cotyledons were embedded in the endosperm or excised and incubated in 100 mM sucrose. If, however, the sucrose solution was replaced by sucrose-free buffer solution, the sucrose levels in the phloem decreased with a kinetic depending on the seedling: in some cases there was a smooth decline, in some a decline followed by a slight recovery and in some cases a clear-cut oscillation. The sucrose concentration was often not identical in the phloem of the individual bundles. The oscillations were larger in the phloem at the apex of the hypocotyl than in the phloem at the base of the hypocotyl. Cutting the petiole of one cotyledon led to a decrease in sucrose not only in the four bundles directly connected to the severed petiole but in all eight bundles of the hypocotyl. Cutting the petiole and dividing the vascular ring at the cotyledonary node and at the root crown did not prevent the decline of sucrose in all eight bundles. Therefore, a functional equilibration of translocated solutes between the eight bundles may occur within the 1-h measuring interval by radial diffusion through the parenchyma of the hypocotyl.

Key words: Node (vasculature) Phloem Ricinus (phloem sucrose) Sieve tube Sucrose deprivation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jutta Verscht
    • 1
  • Bernhard Kalusche
    • 2
  • Jutta Köhler
    • 1
  • Walter Köckenberger
    • 1
  • Alexander Metzler
    • 4
  • Axel Haase
    • 2
  • Ewald Komor
    • 1
  1. 1.Pflanzenphysiologie, Universität Bayreuth, D-95440 Bayreuth, GermanyDE
  2. 2.Experimentelle Physik V, Universität Würzburg, D-97074 Würzburg, GermanyDE
  3. 3.Department of Physics, MR Centre, University of Nottingham, Nottingham NG7 2RD, UKGB
  4. 4.Fachbereich Biologie/Chemie, Universität Bremen, D-28334 Bremen, GermanyDE

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