Planta

, Volume 205, Issue 1, pp 32–38

Stage-specific responses of embryogenic carrot cell suspension cultures to arabinogalactan protein-binding β-glucosyl Yariv reagent

  • Helen J. M. Thompson
  • J. Paul Knox
Article

DOI: 10.1007/s004250050293

Cite this article as:
Thompson, H. & Knox, J. Planta (1998) 205: 32. doi:10.1007/s004250050293

Abstract.

The arabinogalactan protein-binding β-d-glucosyl Yariv reagent (βGlcY) was applied to the various developmental stages of embryogenic carrot (Daucus carota L. cv. Early Nantes) cell-suspension cultures. Roots without shoot structures were produced in cultures grown under embryo-inducing conditions in medium containing βGlcY. Only low concentrations of βGlcY permitted the subsequent production of embryos in these cultures. When early stage embryos were transferred to medium containing βGlcY, the roots elongated greatly while the shoot apices expanded radially. These embryos did not progress to the next developmental stage. Torpedo embryos and plantlets, however, showed an overall inhibition of growth in the presence of βGlcY. Developmental stage therefore appears to determine how cultures and embryos respond to βGlcY, root growth being promoted in the early stages, and overall growth reduced in the late stages.

Key words: Arabinogalactan protein Daucus (arabinogalactan proteins) β-Glucosyl Yariv reagent Cell suspension culture Somatic embryogenesis 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Helen J. M. Thompson
    • 1
  • J. Paul Knox
    • 1
  1. 1.Centre for Plant Biochemistry and Biotechnology, University of Leeds, Leeds, LS2 9JT, UKGB

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