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Planta

, Volume 201, Issue 3, pp 273–280 | Cite as

Formation of a phragmosome-like structure in centrifuged protonemal cells of Adiantum capillus-veneris L.

  • Takashi MurataEmail author
  • Masamitsu Wada
Article

Abstract

A phragmosome (PS) is a transvacuolar aggregation of cytoplasm that develops in the plane of future cytokinesis and is found specifically in highly vacuolated cells. Although protonemal cells of Adiantum capillus-veneris L. usually do not form a PS, a PS-like structure developed at the site of a preprophase band (PPB) of microtubules (MTs) when the nucleus and endoplasm were displaced from the division site by centrifugation, leaving a PPB in the cortical cytoplasm. The PS-like structure contained endoplasmic MTs, Factin, oil droplets and mitochondria. The structure did not develop when the cells were centrifuged before the formation of a PPB. Application of amiprophos-methyl (APM) before development of the PPB strongly inhibited the formation of the PS-like structure after centrifugation. The PS-like structure was dispersed after cytokinesis which occurred in the region of the displaced nucleus. Treatment with APM after the formation of the PS-like structure arrested the cell cycle at the M phase and inhibited the degradation of this structure. These results suggest that development of a PS-like structure is associated both with the formation of a PPB and with the stage of the cell cycle.

Key words

Adiantum Cytokinesis Microtubule Phragmosome Preprophase band Protonema 

Abbreviations

APM

amiprophos-methyl

DAPI

4′,6′-diamidino-2-phenylindole

DMSO

dimethylsulfoxide

MT

microtubule

PPB

preprophase band

PS

phragmosome

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  1. 1.Department of Life Science, Graduate School of Arts and SciencesThe University of TokyoTokyoJapan
  2. 2.Department of Biology, Faculty of ScienceTokyo Metropolitan UniversityTokyoJapan

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