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Planta

, Volume 213, Issue 2, pp 231–240 | Cite as

A xylosyltransferase that synthesizes β-(1→4)-xylans in wheat (Triticum aestivum L.) seedlings

  • H. Kuroyama
  • Y. TsumurayaEmail author
Original Article

Abstract.

A particulate preparation from 6-day-old seedlings of wheat (Triticum aestivum L.) was found to contain a xylosyltransferase (XylTase) which incorporated xylose (Xyl) from UDP-xylose into exogenous β-(1→4)-xylooligosaccharides with 2-aminopyridine-derivatized reducing end groups. High-performance liquid chromatographic analysis showed that the chain elongation of pyridylaminated β-(1→4)-xylotriose (Xyl3-PA) occurred by attachment of a series of one, two, or three xylosyl residues, depending on substrate concentrations and reaction times. Methylation analysis and β-xylosidase digestion of the newly synthesized Xyl4-PA confirmed that the xylosyl residues were incorporated through β-(1→4)-linkages. The enzyme was maximally active at pH 6.8 and 20 °C, and required Triton X-100, which enhanced activity 5-fold at a concentration of 0.05–2%. Divalent ions, including Mn2+ and Mg2+, did not affect activity. Enzyme activity increased with increasing polymerization of xylosyl residues of the acceptor substrates: for instance, Xyl5-PA was almost 7 times as efficient as Xyl2-PA. The apparent Michaelis constants of the enzyme for Xyl3-PA and UDP-xylose were 13.5 and 7.9 mM, respectively. The enzyme also catalyzed incorporation of radioactive sugars (Xyl together with a small portion of L-arabinose) from UDP-[14C]xylose into higher β-(1→4)-xylooligosaccharides (degree of polymerization > 7) with or without (4-O-methyl-)glucuronosyl side chains at activities comparable to those observed for pyridylaminated xylooligosaccharides, and into several heteroxylans but with much lower efficiency. Enzymatic hydrolysis of the product with a β-xylanase degraded it into mainly xylobiose, providing further evidence that the xylosyl residues are incorporated through β-(1→4)-linkages.

Cell wall (xylan) Triticum (cell wall) Xylosyltransferase Xylan 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-okubo, Urawa 338-8570, JapanJapan

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