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Planta

, Volume 211, Issue 6, pp 887–893 | Cite as

A novel reverse-genetic approach (SIMF) identifies Mutator insertions in new Myb genes

  • Pablo D. Rabinowicz
  • Erich Grotewold
Rapid communication

Abstract.

We have developed a new strategy designated SIMF (Systematic Insertional Mutagenesis of Families), to identify DNA insertions in many members of a gene family simultaneously. This method requires only a short amino acid sequence conserved in all members of the family to make a degenerate oligonucleotide, and a sequence from the end of the DNA insertion. The SIMF strategy was successfully applied to the large maize R2R3 Myb family of regulatory genes, and Mutator insertions in several novel Myb genes were identified. Application of this technique to identify insertions in other large gene families could significantly decrease the effort involved in screening at the same time for insertions in all members of groups of genes that share a limited sequence identity.

Key words: DNA insertions (identification) – Myb – Transposable element – Transcription factor –Zea (DNA insertions) 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Pablo D. Rabinowicz
    • 2
  • Erich Grotewold
    • 1
  1. 1.Department of Plant Biology and Plant Biotechnology Center, The Ohio State University, Columbus, OH 43210, USAUS
  2. 2.Cold Spring Harbor Laboratory, 1 Bungtown Rd., Cold Spring Harbor, NY 11724, USAUS

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