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Planta

, 251:16 | Cite as

Oxidation of polyphenols and inhibition of photosystem II under acute photooxidative stress

  • Guy SamsonEmail author
  • Zoran G. Cerovic
  • Waleed M. A. El Rouby
  • Pierre Millet
Original Article
  • 50 Downloads

Abstract

Main conclusion

We observed a close correlation between the inhibition of photosystem II and the oxidation of polyphenols during an acute oxidative stress in sunflower leaf discs.

Abstract

To assess the physiological significance of polyphenols as antioxidants in planta, we compared the kinetics of polyphenols oxidation with the inhibition of the photosynthetic apparatus in sunflower leaf discs exposed to an acute photooxidative stress. Illumination of leaf discs in the presence of methyl viologen induced a rapid and large non-photochemical quenching of chlorophyll-a fluorescence, which was reversed after 4 h of treatment as indicated by the ≈ 30% increases of the steady-state (Fs) and maximal (Fm′) levels of chlorophyll-a fluorescence relative to the first hour of treatment. This event coincided with the accelerated decreases of the maximum (Fv/Fm) and effective (∆F/Fm′) quantum yields of photosystem II, and also with the beginning of polyphenols oxidation, estimated by the UV absorbance of methanolic leaf extracts, and supported by the Folin–Ciocalteu method and cyclic voltammetry. The decreases of Fv/Fm and the concentrations of reducing polyphenols were highly correlated (R2 = 0.877) during the experiment. Coherent with the decrease of UV absorbance of methanolic extracts, polyphenol oxidation resulted in a marked decrease of UV absorbance of leaf epidermis. Also, polymerization of oxidized polyphenols caused the accumulation of brown pigments in the MV-treated leaf discs, decreasing leaf reflectance, especially at 550 and 740 nm. Fluorescence intensities were also decreased during the MV treatment. Interestingly, the emission fluorescence ratio F740/F684 (excitation at 550 nm) decreased similarly to Fv/Fm (R2 = 0.981) due to the brown pigments. Moreover, the excitation fluorescence ratio F484/F680 (emission at 740 nm) was linearly correlated (R2 = 0.957) to ∆F/Fm′, indicating a decrease of efficiency of energy transfer between the antenna pigments to the photosystem II reaction center during the oxidative stress. These results support the view that polyphenols can be effective antioxidants protecting the plants against reactive oxygen species.

Keywords

Antioxidants Brown pigments Chlorophyll fluorescence Excitation spectra Reflectance spectra Helianthus annuus Photosynthesis Polyphenols 

Notes

Acknowledgements

We are grateful to Lionel Saunois and Amandine Dubois from the greenhouse facility for taking care of the sunflower plants, and to Gwendal Latouche for his technical assistance. GS acknowledges his sabbatical leave from the Université du Québec à Trois-Rivières.

Author contribution statement

GS and ZGC conceived research, conducted most experiments and analyzed the data. WMAER and PM conducted experiments with voltammetry and analyzed the data. GS prepared the figures and wrote most of the manuscript. All authors reviewed and approved the manuscript.

Compliance with ethical standards

Conflict of interest

ZGC declares a link to the company FORCE-A as one of the co-authors of the Multiplex patent that the company exploits. Other authors have no competing interests.

Supplementary material

425_2019_3316_MOESM1_ESM.docx (57 kb)
Fig. 1S Polyphenol oxidation estimated from leaf methanolic extracts. Absorbance spectra of extracts from leaf treated or not with methyl viologen for different times (DOCX 56 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Guy Samson
    • 1
    Email author
  • Zoran G. Cerovic
    • 2
  • Waleed M. A. El Rouby
    • 3
    • 4
  • Pierre Millet
    • 3
  1. 1.Groupe de Recherche en Biologie Végétale, Département des sciences de l’environnementUniversité du Québec à Trois-RivièresTrois-RivièresCanada
  2. 2.Écologie, Systématique et Évolution, UMR 8079, CNRS, Univ. Paris-Sud, AgroParisTechUniversité Paris-SaclayOrsayFrance
  3. 3.Institut de Chimie Moléculaire et des Matériaux d’Orsay, UMR 8182, Université Paris-SudOrsay cedexFrance
  4. 4.Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Science (PSAS)Beni-Suef UniversityBeni-SuefEgypt

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