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Planta

, Volume 250, Issue 6, pp 1911–1925 | Cite as

Identification of Shaker K+ channel family members in Rosaceae and a functional exploration of PbrKAT1

  • Guodong Chen
  • Qian Chen
  • Kaijie Qi
  • Zhihua Xie
  • Hao Yin
  • Peng Wang
  • Runze Wang
  • Zhi Huang
  • Shaoling Zhang
  • Li WangEmail author
  • Juyou WuEmail author
Original Article

Abstract

Main conclusion

PbrKAT1, which is inhibited by external Na+ in Xenopus laevis oocytes, is characterized as encoding a typical inward rectifying channel that is mainly expressed in guard cells.

Abstract

Potassium (K+) is the most abundant cation in plant cells necessary for plant growth and development. The uptake and transport of K+ are mainly completed through transporters and channels, and the Shaker family genes are the most studied K+ channels in plants. However, there is far less information about this family in Rosaceae species. We performed a genome-wide analysis and identified Shaker K+ channel gene family members in Rosaceae. We cloned and characterized a Shaker K+ channel KAT1 from pear (Pyrus × bretschneideri). In total, 36 Shaker K+ channel genes were identified from Rosaceae species and were classified into five subgroups based on structural characteristics and a phylogenetic analysis. Whole-genome and dispersed duplications were the primary forces underlying Shaker K+ channel gene family expansion in Rosaceae, and purifying selection played a key role in the evolution of Shaker K+ channel genes. β-Glucuronidase and qRT-PCR assays revealed that PbrKAT1 was mainly expressed in leaves, especially in guard cells. PbrKAT1 displayed a typical inward-rectifying current when expressed in Xenopus laevis oocytes. The activity of PbrKAT1 was inhibited by external sodium ions, possibly playing an important role in the regulation of salt tolerance in pear. These results provide valuable information on evolution, expression and functions of the Shaker K+ channel gene family in plants.

Keywords

Na+ inhibition Pear Potassium Stomata Voltage-clamp technique 

Abbreviations

GUS

β-Glucuronidase

Kin

The inward rectifying K+

WGD

Whole Genome Duplication

Notes

Funding

This work was supported by the Fundamental Research Funds for the Central Universities (KJQN201926), National Natural Science Foundation of China (31801842, 31772256), and the National Key R&D Program of China (2018YFD0201400).

Compliance with ethical standards

Competing interests

The authors declare that no competing interests exist.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Guodong Chen
    • 1
  • Qian Chen
    • 1
  • Kaijie Qi
    • 1
  • Zhihua Xie
    • 1
  • Hao Yin
    • 1
  • Peng Wang
    • 1
  • Runze Wang
    • 1
  • Zhi Huang
    • 1
  • Shaoling Zhang
    • 1
  • Li Wang
    • 1
    Email author
  • Juyou Wu
    • 1
    Email author
  1. 1.Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm EnhancementCollege of Horticulture, Nanjing Agricultural UniversityNanjingChina

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