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Planta

, Volume 250, Issue 6, pp 1897–1910 | Cite as

The Rpf84 gene, encoding a ribosomal large subunit protein, RPL22, regulates symbiotic nodulation in Robinia pseudoacacia

  • Zhao Feng
  • Lu Zhang
  • Yuanyuan Wu
  • Li Wang
  • Mingying Xu
  • Mo Yang
  • Yajuan Li
  • Gehong Wei
  • Minxia ChouEmail author
Original Article

Abstract

Main conclusion

A homologue of the ribosomal protein L22e, Rpf84, regulates root nodule symbiosis by mediating the infection process of rhizobia and preventing bacteroids from degradation in Robinia pseudoacacia.

Abstract

Ribosomal proteins (RPs) are known to have extraribosomal functions, including developmental regulation and stress responses; however, the effects of RPs on symbiotic nodulation of legumes are still unclear. Ribosomal protein 22 of the large 60S subunit (RPL22), a non-typical RP that is only found in eukaryotes, has been shown to function as a tumour suppressor in animals. Here, a homologue of RPL22, Rpf84, was identified from the leguminous tree R. pseudoacacia. Subcellular localization assays showed that Rpf84 was expressed in the cytoplasm and nucleus. Knockdown of Rpf84 by RNA interference (RNAi) technology impaired the infection process and nodule development. Compared with the control, root and stem length, dry weight and nodule number per plant were drastically decreased in Rpf84-RNAi plants. The numbers of root hair curlings, infection threads and nodule primordia were also significantly reduced. Ultrastructure analyses showed that Rpf84-RNAi nodules contained fewer infected cells with fewer bacteria. In particular, remarkable deformation of bacteroids and fusion of multiple symbiosomes occurred in infected cells. By contrast, overexpression of Rpf84 promoted nodulation, and the overexpression nodules maintained a larger infection/differentiation region and had more infected cells filled with bacteroids than the control at 45 days post inoculation, suggesting a retarded ageing process in nodules. These results indicate for the first time that RP regulates the symbiotic nodulation of legumes and that RPL22 may function in initiating the invasion of rhizobia and preventing bacteroids from degradation in R. pseudoacacia.

Keywords

Black locust Infection process Nodule symbiosis Ribosomal protein Subcellular location Symbiosome 

Abbreviations

Dpi

Days post-inoculation

GFP

Green fluorescent protein

GUS

β-Glucuronidase

IT

Infection thread

Lb

Leghaemoglobin

RNAi

RNA interference

RP

Ribosomal protein

RPL

Ribosomal large subunit protein

TEM

Transmission electron microscopy

Notes

Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (Grant no. 2016YFD0800706), the National Natural Science Foundation of China (Grant no. 31172252) and the Shaanxi Province Natural Science Foundation of China (Grant no. 2016JM3004).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

425_2019_3267_MOESM1_ESM.pdf (182 kb)
Supplementary material 1 (PDF 181 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Stress Biology in Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life SciencesNorthwest A&F UniversityYanglingChina
  2. 2.College of Medical TechnologyShaanxi University of Chinese MedicineXianyangChina

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