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Ionic selectivity and coordinated transport of Na+ and K+ in flag leaves render differential salt tolerance in rice at the reproductive stage

  • Koushik ChakrabortyEmail author
  • Krishnendu ChattaopadhyayEmail author
  • Lopamudra Nayak
  • Soham Ray
  • Lucina Yeasmin
  • Priyanka Jena
  • Sunanda Gupta
  • Sangram K. Mohanty
  • Padmini Swain
  • Ramani K. Sarkar
Original Article

Abstract

Main conclusion

The present study shows that salt tolerance in the reproductive stage of rice is primarily governed by the selective Na+ and K+ transport from the root to upper plant parts. Ionic discrimination at the flag leaf, governed by differential expression of Na+- and K+-specific transporters/ion pumps, is associated with reduced spikelet sterility and reproductive stage salt tolerance.

Abstract

Reproductive stage salt tolerance is crucial in rice to guarantee yield under saline condition. In the present study, differential ionic selectivity and the coordinated transport (from root to flag leaf) of Na+ and K+ were investigated to assess their impact on reproductive stage salt tolerance. Four rice genotypes having differential salt sensitivity were subjected to reproductive stage salinity stress in pots. The selective Na+ and K+ transport from the root to upper plant parts was observed in tolerant genotypes. We noticed that prolonged salt exposure did not alter flag leaf greenness even up to 6 weeks; however, it had a detrimental effect on panicle development especially in the salt-susceptible genotype Sabita. But more precise chlorophyll fluorescence imaging analysis revealed salinity-induced damages in Sabita. The salt-tolerant genotype Pokkali (AC41585), a potential Na+ excluder, managed to sequester higher Na+ load in the roots with little upward transport as evident from greater expression of HKT1 and HKT2 transporters. In contrast, the moderately salt-tolerant Lunidhan was less selective in Na+ transport, but possessed a higher capacity to Na+ sequestration in leaves. Higher K+ uptake and tissue-specific redistribution mediated by HAK and AKT transporters showed robust control in selective K+ movement from the root to flag leaf and developing panicles. On the contrary, expressions of Na+-specific transporters in developing panicles were either down-regulated or unaffected in tolerant and moderately tolerant genotypes. Yet, in the panicles of the susceptible genotype Sabita, some of the Na+-specific transporter genes (SOS1, HKT1;5, HKT2;4) were upregulated. Apart from the ionic regulation strategy, cellular energy balance mediated by different plasma-membrane and tonoplastic H+-pumps were also associated with the reproductive stage salt tolerance in rice.

Keywords

Chlorophyll fluorescence imaging Flowering stage Ion channels Ion pumps Salinity Transporter 

Abbreviations

ChlF

Chlorophyll fluorescence

HKT

High-affinity potassium transporter

SOS

Salt overly sensitive

SSI

Stress stability index

V-PPase

Vacuolar pyrophosphatase

V-ATPase

Vacuolar type H+ ATPase

YSI

Yield stability index

Notes

Acknowledgements

We thank ICAR-National Rice Research Institute, Cuttack, Odisha, India, and National Innovations on Climate Resilient Agriculture (NICRA, EAP-245), Indian Council of Agricultural Research, New Delhi, for providing necessary facilities and funding to carry out the research.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

Supplementary material

425_2019_3253_MOESM1_ESM.pdf (236 kb)
Supplementary material 1 (PDF 236 kb)
425_2019_3253_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 15 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.ICAR, National Rice Research InstituteCuttackIndia

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