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SCARECROW promoter-driven expression of a bacterial mercury transporter MerC in root endodermal cells enhances mercury accumulation in Arabidopsis shoots

  • Shimpei Uraguchi
  • Yuka Sone
  • Aino Yoshikawa
  • Michi Tanabe
  • Haruka Sato
  • Yuto Otsuka
  • Ryosuke Nakamura
  • Yasukazu Takanezawa
  • Masako KiyonoEmail author
Short Communication

Abstract

Main conclusion

Mercury accumulation in Arabidopsis shoots is accelerated by endodermis specific expression of fusion proteins of a bacterial mercury transporter MerC and a plant SNARE SYP121 under control of SCARECROW promoter.

Abstract

We previously demonstrated that the CaMV 35S RNA promoter (p35S)-driven ubiquitous expression of a bacterial mercury transporter MerC, fused with SYP121, an Arabidopsis SNARE protein increases mercury accumulation of Arabidopsis. To establish an improved fine-tuned mercury transport system in plants for phytoremediation, the present study generated and characterized transgenic Arabidopsis plants expressing MerC-SYP121 specifically in the root endodermis, which is a crucial cell type for root element uptake. We generated four independent transgenic Arabidopsis lines expressing a transgene encoding mCherry-MerC-SYP121 under the control of the endodermis-specific SCARECROW promoter (hereafter pSCR lines). Quantitative real-time PCR analysis showed that expression levels of the transgene in roots of the pSCR lines were 3–23% of the p35S driven-overexpressing line. Confocal microscopy analysis showed that mCherry-MerC-SYP121 was dominantly expressed in the endodermis of the meristematic zone as well as in the mature zone of the pSCR roots. Mercury accumulation in shoots of the pSCR lines exposed to inorganic mercury was overall higher than the wild-type and comparable to the p35S over-expressing line. These results suggest that endodermis-specific expression of the MerC-SYP121 fusion proteins in plant roots sufficiently enhances mercury uptake and accumulation into shoots, which would be an ideal phenotype for phytoremediation of mercury-contaminated environments.

Keywords

Bacterial mercury transporter Cell-type specificity Endodermis MerC Phytoremediation SCARECROW 

Abbreviations

pSCR

SCARECROW promoter

SNARE

Soluble N-ethylmaleimide-sensitive factor protein attachment protein receptor

Notes

Acknowledgements

We thank Prof. Dr. Angelika Mustroph (University of Bayreuth) for providing a plasmid containing the pSCR fragment. This work was partly supported by the Japan Society for the Promotion of Science (Grant nos. 15H02839 and 18H03401 to M.K.).

Supplementary material

425_2019_3186_MOESM1_ESM.pdf (746 kb)
Supplementary material 1 (PDF 746 kb)
425_2019_3186_MOESM2_ESM.xlsx (10 kb)
Supplementary material 2 (XLSX 9 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Shimpei Uraguchi
    • 1
  • Yuka Sone
    • 1
  • Aino Yoshikawa
    • 1
  • Michi Tanabe
    • 1
  • Haruka Sato
    • 1
  • Yuto Otsuka
    • 1
  • Ryosuke Nakamura
    • 1
  • Yasukazu Takanezawa
    • 1
  • Masako Kiyono
    • 1
    Email author
  1. 1.Department of Public Health, School of PharmacyKitasato UniversityTokyoJapan

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