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pp 1–9 | Cite as

Speciation and accumulation of Zn in sweetcorn kernels for genetic and agronomic biofortification programs

  • Zhong Xiang CheahEmail author
  • Peter M. Kopittke
  • Stephen M. Harper
  • Gregor Meyer
  • Tim J. O’Hare
  • Michael J. Bell
Original Article

Abstract

Main conclusion

In sweetcorn (Zea mays L.), embryo Zn is accumulated mainly as Zn-phytate, whereas endosperm Zn is complexed with a N- or S-containing ligand.

Understanding the speciation of Zn in crop plants helps improve the effectiveness of biofortification efforts. Kernels of four sweetcorn (Zea mays L.) varieties were analysed for Zn concentration and content. We also assessed the speciation of the Zn in the embryo, endosperm, and pericarp in situ using synchrotron-based X-ray absorption spectroscopy. The majority of the Zn was in the endosperm and pericarp (72%), with the embryo contributing 28%. Approximately 79% of the Zn in the embryo accumulated as Zn-phytate, whereas in the endosperm most of the Zn was complexed with a N- or S-containing ligand, possibly as Zn-histidine and Zn-cysteine. This suggests that whilst the Zn in the endosperm and pericarp is likely to be bioavailable for humans, the Zn in the embryo is of low bioavailability. This study highlights the importance of targeting the endosperm of sweetcorn kernels as the tissue for increasing bioavailable Zn concentration.

Keywords

Bioavailability Embryo Endosperm Nutrient Phytate Synchrotron-based X-ray absorption spectroscopy (XAS) Zea mays Zinc 

Abbreviations

DAP

Days after pollination

DW

Dry weight

EXAFS

Extended X-ray absorption fine-structure

ICP-OES

Inductively coupled plasma optical emission spectroscopy

LCF

Linear combination fitting

pKa

Acid dissociation constants

XANES

X-ray absorption near-edge structure

XAS

X-ray absorption spectroscopy

Notes

Acknowledgements

We thank Jeremy Wykes of Australian Synchrotron XAS beamline for assistance provided during data collection.

Author contributions

ZXC, PMK, SMH, TJO and MJB conceived and designed the research. ZXC, PMK, SMH, GM and MJB conducted experiments. ZXC and PMK contributed reagents and experimental tools. ZXC, PMK, SMH, GM and MJB analysed the data. ZXC wrote the manuscript. All the authors read and approved the manuscript.

Funding

This research was undertaken on the X-ray absorption spectroscopy (XAS) beamline at the Australian Synchrotron, part of ANSTO. This work was supported by the Australian Government Research Training Program (RTP) Scholarship and the Australian Centre for International Agricultural Research (ACIAR) [SMCN/2012/029].

Compliance with ethical standards

Conflicts of interest

The authors declare no competing financial interest.

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Agriculture and Food SciencesThe University of QueenslandGattonAustralia
  2. 2.Department of Agriculture and FisheriesGattonAustralia
  3. 3.Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandGattonAustralia

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