, Volume 249, Issue 5, pp 1267–1284 | Cite as

Stress-responsive miRNAome of Glycine max (L.) Merrill: molecular insights and way forward

  • S. V. RameshEmail author
  • V. Govindasamy
  • M. K. Rajesh
  • A. A. Sabana
  • Shelly Praveen


Main conclusion

Analysis of stress-associated miRNAs of Glycine max (L.) Merrill reveals wider ramifications of small RNA-mediated (conserved and legume-specific miRNAs) gene regulatory foot prints in molecular adaptive responses.

MicroRNAs (miRNAs) are indispensable components of gene regulatory mechanism of plants. Soybean is a crop of immense commercial potential grown worldwide for its edible oil and soy meal. Intensive research efforts, using the next generation sequencing and bioinformatics techniques, have led to the identification and characterization of numerous small RNAs, especially microRNAs (miRNAs), in soybean. Furthermore, studies have unequivocally demonstrated the significance of miRNAs during the developmental processes and various stresses in soybean. In this review, we summarize the current state of understanding of miRNA-based abiotic and biotic stress responses in soybean. In addition, the molecular insights gained from the stress-related soybean miRNAs have been compared to the miRNAs of other crops, especially legumes, and the core commonalities have been highlighted, though differences among them were not ignored. Nature of response of soybean-derived conserved miRNAs during various stresses was also analyzed to gain deeper insights regarding sRNAome-based defense responses. This review further provides way forward in legume small RNA transcriptomics based on the adaptive responses of soybean and other legume-derived miRNAs.


Conserved miRNAs Gene regulation Legumes miRNA evolution Non-coding RNAs Soybean Stressors 





Arbuscular mycorrhiza




Auxin response factor


Asian soybean rust




Dehydration responsive element


Early nodulin 93


Genome survey sequence


Heterochromatic siRNAs


HUA enhancer 1










Natural antisense transcript siRNAs


Next generation sequencing


Protein encoding genes


Post transcriptional gene silencing


dsRNA-binding proteins


RNA-dependent DNA methylation


RNA-induced silencing complex


Soybean cyst nematode




Small interfering RNAs


Soybean mosaic virus


Small non-coding RNAs


Symbiotic nitrogen fixation


Transcriptional factors


Transcriptional gene silencing



This study was funded by Indian Council of Agricultural Research (ICAR)-Indian Institute of Soybean Research (ICAR-IISR) sponsored project (Grant no.: 1.24/12).

Compliance with ethical standards

Conflict of interest

Authors declare that there are no competing interests.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.ICAR-Indian Institute of Soybean Research (ICAR-IISR)IndoreIndia
  2. 2.ICAR-Central Plantation Crops Research Institute (ICAR-CPCRI)KasaragodIndia
  3. 3.ICAR-Indian Agricultural Research Institute (ICAR-IARI)New DelhiIndia

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