Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development
- 24 Downloads
Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism.
Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.
KeywordsArabinofuranosidase Cuticle Post-genital fusion Rhamnogalacturonan-I Xyloglucan
Cotton fibre middle lamella
Comprehensive microarray polymer profiling
This work was supported by Villum Foundation project PLANET (Grant No. 00009283) and the European Union Seventh Framework Programme under the WallTraC project (Grant agreement No. 263916). This paper reflects the authors’ views only. The European Community is not liable for any use that may be made of the information contained herein. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Ademe MS, He S, Pan Z, Sun J, Wang Q, Qin H, Liu J, Liu H, Yang J, Xu D, Yang J, Ma Z, Zhang J, Li Z, Cai Z, Zhang X, Zhang X, Huang A, Yi X, Zhou G, Li L, Zhu H, Pang B, Wang L, Jia Y, Du X (2017) Association mapping analysis of fiber yield and quality traits in upland cotton (Gossypium hirsutum L.). Mol Genet Genom 292:1267–1280. https://doi.org/10.1007/s00438-017-1346-9 CrossRefGoogle Scholar
- Buffetto F, Cornuault V, Rydahl MG, Ropartz D, Alvarado C, Echasserieau V, Le Gall S, Bouchet B, Tranquet O, Verhertbruggen Y, Willats WGT, Knox JP, Ralet MC, Guillon F (2015) The deconstruction of pectic rhamnogalacturonan I unmasks the occurrence of a novel arabinogalactan oligosaccharide epitope. Plant Cell Physiol 56:2181–2196Google Scholar
- Hande AS, Katageri IS, Jadhav MP, Adiger S, Gamanagatti S, Padmalatha KV, Dhandapani G, Kanakachari M, Kumar PA, Reddy VS (2017) Transcript profiling of genes expressed during fibre development in diploid cotton (Gossypium arboreum L.). BMC Genom 18:675. https://doi.org/10.1186/s12864-017-4066-y CrossRefGoogle Scholar
- Hernandez-Gomez MC, Runavot JL, Guo XY, Bourot S, Benians TAS, Willats WGT, Meulewaeter F, Knox JP (2015a) Heteromannan and heteroxylan cell wall polysaccharides display different dynamics during the elongation and secondary cell wall deposition phases of cotton fiber cell development. Plant Cell Physiol 56:1786–1797CrossRefGoogle Scholar
- Mishra DK, Agrawa N, Choudhary A, Yadav VK, Yadav VK (2018) An overview on advances in cotton genome and regulation of fiber development. ISJR 7:294–300Google Scholar
- Moller I, Marcus SE, Haeger A, Verhertbruggen Y, Verhoef R, Schols H, Ulvskov P, Mikkelsen JD, Knox JP, Willats W (2008) High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconj J 25:37–48CrossRefGoogle Scholar
- Pedersen HL, Fangel JU, McCleary B, Ruzanski C, Rydahl MG, Ralet MC, Farkas V, von Schantz L, Marcus SE, Andersen MC, Field R, Ohlin M, Knox JP, Clausen MH, Willats WG (2012) Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem 287:39429–39438. https://doi.org/10.1074/jbc.M112.396598 CrossRefGoogle Scholar
- Yuan D, Tang Z, Wang M, Gao W, Tu L, Jin X, Chen L, He Y, Zhang L, Zhu L, Li Y, Liang Q, Lin Z, Yang X, Liu N, Jin S, Lei Y, Ding Y, Li G, Ruan X, Ruan Y, Zhang X (2015) The genome sequence of Sea-Island cotton (Gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres. Nat Sci Rep 5:17662. https://doi.org/10.1038/srep17662 CrossRefGoogle Scholar