pp 1–13 | Cite as

Cultures of Gossypium barbadense cotton ovules offer insights into the microtubule-mediated control of fiber cell expansion

  • Ethan T. Pierce
  • Benjamin P. Graham
  • Michael R. Stiff
  • Jason A. Osborne
  • Candace H. HaiglerEmail author
Original Article


Main conclusion

A novel method for culturing ovules of Gossypium barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion.

Cotton fibers undergo extensive elongation and secondary wall thickening as they develop into our most important renewable textile material. These single cells elongate at the apex as well as elongating and expanding in diameter behind the apex. These multiple growth modes represent an interesting difference compared to classical tip-growing cells that needs to be explored further. In vitro ovule culture enables experimental analysis of the controls of cotton fiber development in commonly grown Gossypium hirsutum cotton, but, previously, there was no equivalent system for G. barbadense, which produces higher quality cotton fiber. Here, we describe: (a) how to culture the ovules of G. barbadense successfully, and (b) the results of an in vitro experiment comparing the role of microtubules in controlling cell expansion in different zones near the apex of three types of cotton fiber tips. Adding the common herbicide fluridone, 1-Methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone, to the medium supported G. barbadense ovule culture, with positive impacts on the number of useful ovules and fiber length. The effect is potentially mediated through inhibited synthesis of abscisic acid, which antagonized the positive effects of fluridone. Fiber development was perturbed by adding colchicine, a microtubule antagonist, to ovules of G. barbadense and G. hirsutum cultured 2 days after flowering. The results supported the zonal control of cell expansion in one type of G. hirsutum fiber tip and highlighted differences in the role of microtubules in modulating cell expansion between three types of cotton fiber tips.


Colchicine Cotton fiber Cytoskeleton Fluridone Ovule culture Plant cell growth 



Abscisic acid


Analysis of variance


Days post-anthesis


Dimethyl sulfoxide


Gossypium barbadense


Gossypium hirsutum


Honestly significant difference



For research support, we thank Cotton Incorporated, Cary, NC. For assistance with fiber tip measurements, we thank Robin Grant Moore and Anne Pajerski. For assistance with laser scanning confocal microscopy, we thank Dr. Eva Johannes in the Cellular and Molecular Imaging Facility of North Carolina State University.

Compliance with ethical standards

Conflicts of Interest

The authors state that they have no conflict of interest.

Supplementary material

425_2019_3106_MOESM1_ESM.pdf (2.8 mb)
Supplementary material 1 (pdf 2830 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ethan T. Pierce
    • 1
    • 2
  • Benjamin P. Graham
    • 2
  • Michael R. Stiff
    • 1
    • 4
  • Jason A. Osborne
    • 3
  • Candace H. Haigler
    • 1
    • 2
    Email author
  1. 1.Department of Crop and Soil SciencesNorth Carolina State UniversityRaleighUSA
  2. 2.Department of Plant and Microbial BiologyNorth Carolina State UniversityRaleighUSA
  3. 3.Department of StatisticsNorth Carolina State UniversityRaleighUSA
  4. 4.Department of BiologyLenoir-Rhyne UniversityHickoryUSA

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