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Gene expression and spatiotemporal localization of antifungal chitin-binding proteins during Moringa oleifera seed development and germination

  • Tarcymara B. Garcia
  • Arlete A. Soares
  • Jose H. Costa
  • Helen P. S. Costa
  • João X. S. Neto
  • Lady Clarissa B. Rocha-Bezerra
  • Fredy Davi A. Silva
  • Mariana R. Arantes
  • Daniele O. B. Sousa
  • Ilka M. VasconcelosEmail author
  • Jose T. A. OliveiraEmail author
Original Article
First Online:
  • 64 Downloads

Abstract

Main conclusion

Chitin-binding proteins behave as storage and antifungal proteins in the seeds of Moringa oleifera.

Moringa oleifera is a tropical multipurpose tree. Its seed constituents possess coagulant, bactericidal, fungicidal, and insecticidal properties. Some of these properties are attributed to a group of polypeptides denominated M. oleifera chitin-binding proteins (in short, Mo-CBPs). Within this group, Mo-CBP2, Mo-CBP3, and Mo-CBP4 were previously purified to homogeneity. They showed high amino acid similarity with the 2S albumin storage proteins. These proteins also presented antimicrobial activity against human pathogenic yeast and phytopathogenic fungi. In the present study, the localization and expression of genes that encode Mo-CBPs and the biosynthesis and degradation of the corresponding proteins during morphogenesis and maturation of M. oleifera seeds at 15, 30, 60, and 90 days after anthesis (DAA) and germination, respectively, were assessed. The Mo-CBP transcripts and corresponding proteins were not detected at 15 and 30 days after anthesis (DAA). However, they accumulated at the latter stages of seed maturation (60 and 90 DAA), reaching the maximum level at 60 DAA. The degradation kinetics of Mo-CBPs during seed germination by in situ immunolocalization revealed a reduction in the protein content 48 h after sowing (HAS). Moreover, Mo-CBPs isolated from seeds at 60 and 90 DAA prevented the spore germination of Fusarium spp. Taken together, these results suggest that Mo-CBPs play a dual role as storage and defense proteins in the seeds of M. oleifera.

Keyword

Drumstick seed Mo-CBPs 2S albumin Biosynthesis Degradation Antifungal activity 
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Notes

Acknowledgments

This work was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazilian Ministry of Education, Process Number: 306202/2017-4, grant number 1280942/2014-2018); the CAPES - Toxinology Project, Process Number: 431511/2016-0; and the Brazilian National Council for Scientific and Technological Development (CNPq, Process number: 306202/2017-4). We are also grateful to the Central Analytical facilities of the Federal University of Ceara, Brazil, for the scanning electron micrograph (SEM) analysis.

Compliance with ethical standards

Conflict of interest

The authors hereby declare no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Tarcymara B. Garcia
    • 1
  • Arlete A. Soares
    • 2
  • Jose H. Costa
    • 1
  • Helen P. S. Costa
    • 1
  • João X. S. Neto
    • 1
  • Lady Clarissa B. Rocha-Bezerra
    • 1
  • Fredy Davi A. Silva
    • 1
  • Mariana R. Arantes
    • 1
  • Daniele O. B. Sousa
    • 1
  • Ilka M. Vasconcelos
    • 1
    Email author
  • Jose T. A. Oliveira
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyFederal University of CearaFortalezaBrazil
  2. 2.Department of BiologyFederal University of CearaFortalezaBrazil

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