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Planta

, Volume 249, Issue 2, pp 515–525 | Cite as

Knockdown of PCBER1, a gene of neolignan biosynthesis, resulted in increased poplar growth

  • Tobias BruegmannEmail author
  • Hendrik Wetzel
  • Kay Hettrich
  • Annika Smeds
  • Stefan Willför
  • Birgit Kersten
  • Matthias Fladung
Original Article

Abstract

Main conclusion

Poplar trees displayed an increased plant height due to the transgenic knockdown of PCBER1, a gene of lignan biosynthesis. The wood composition was slightly altered in both overexpression and knockdown lines.

The gene PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE1 (PCBER1) is well known as an important gene in the synthesis of lignans, a group of diverse phenylpropanoid derivatives. They are widely distributed in the plant kingdom and may have a role in both plant defense and growth regulation. To analyze its role in biomass formation and wood composition in poplar, both overexpression and knockdown approaches have been performed. Transgenic lines were analyzed on genetic and phenotypic levels, and partly in regard to their biomass composition. While the PCBER1 overexpression approach remained unremarkable concerning the plant height, biomass composition of obtained transgenic lines was modified. They had a significantly increased amount of ethanol extractives. The PCBER1 knockdown resulted in significantly deviating plants; after 17 months of greenhouse cultivation, transgenic plants were up to 38% higher compared to non-transgenic wild type. Most examined transgenic lines did not reveal a significantly enhanced stem diameter after three vegetation periods in the greenhouse. Significant changes were not obtained with regard to the three major wood components, lignin, cellulose and hemicelluloses. As a slight but not significant reduction in ethanol extractives was detected, the hypothesis arises that the lignan content could be influenced. Lignans become important in the pharmaceutical industry and clinical studies concerning cancer and other diseases, thus further investigations on lignan formation in poplar and its connection to biomass formation seem promising.

Keywords

Biomass Lignan synthesis Lignin Populus Transformation Vegetative growth 

Notes

Acknowledgements

We are grateful to Guido Jach (Phytowelt, Cologne, Germany) for providing the amiRNA transformation vectors. Thanks to Susanne Jelkmann, Olaf Polak, Jonas Schönfeld, Jakob Fromme, Gundel Wiemann, Monika Spauszus, and Rainer Ebbinghaus for technical assistance. This work was part of the joint project “PopMass”, founded by the German Federal Ministry of Education and Research (BMBF) under the funding number 0315972A.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Thuenen Institute of Forest GeneticsGrosshansdorfGermany
  2. 2.Fraunhofer Institute for Applied Polymer ResearchPotsdam-GolmGermany
  3. 3.Johan Gadolin Process Chemistry CentreÅbo Akademi UniversityTurku/ÅboFinland

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