Magnetic sensitivity mediated by the Arabidopsis blue-light receptor cryptochrome occurs during flavin reoxidation in the dark
Arabidopsis cryptochrome mediates responses to magnetic fields that have been applied in the absence of light, consistent with flavin reoxidation as the primary detection mechanism.
Cryptochromes are highly conserved blue-light-absorbing flavoproteins which have been linked to the perception of electromagnetic stimuli in numerous organisms. These include sensing the direction of the earth’s magnetic field in migratory birds and the intensity of magnetic fields in insects and plants. When exposed to light, cryptochromes undergo flavin reduction/reoxidation redox cycles leading to biological activation which generate radical pairs thought to be the basis for magnetic sensitivity. However, the nature of the magnetically sensitive radical pairs and the steps at which they act during the cryptochrome redox cycle are currently a matter of debate. Here, we investigate the response of Arabidopsis cryptochrome-1 in vivo to a static magnetic field of 500 μT (10 × earth’s field) using both plant growth and light-dependent phosphorylation as an assay. Cryptochrome responses to light were enhanced by the magnetic field, as indicated by increased inhibition of hypocotyl elongation and increased cryptochrome phosphorylation. However, when light and dark intervals were given intermittently, a plant response to the magnetic field was observed even when the magnetic field was given exclusively during the dark intervals between light exposures. This indicates that the magnetically sensitive reaction step in the cryptochrome photocycle must occur during flavin reoxidation, and likely involves the formation of reactive oxygen species.
KeywordsBlue-light photoreceptor Cryptochromes Magnetoreceptor Photomorphogenesis Radical Pairs ROS
Cryptochromes are highly conserved flavoprotein blue-light receptors implicated in numerous aspects of plant growth and development including seedling de-etiolation, response to stress, the initiation of flowering, leaf morphology, elongation growth, and the entrainment of the circadian clock (Chaves et al. 2011; Ozturk 2017; Yang et al. 2017). Structurally, cryptochromes are flavoproteins that undergo light-induced photoreduction, whereby a bound FAD cofactor is reduced from the dark-adapted, oxidized redox state (FADox) to the radical (FADH°), and reduced (FADH-) redox states upon illumination. Formation of the reduced redox state has been correlated with biological activity in numerous studies (Banerjee et al. 2007; Bouly et al. 2007; Kondoh et al. 2011; see review of current literature in Ahmad 2016) as a consequence of conformational change leading to the active state. The reduced flavin cofactor is subsequently oxidized in the presence of molecular oxygen to restore the resting, dark-adapted stage in a reoxidation reaction that does not require light (Muller and Ahmad 2011). In sum, under conditions of illumination, the cryptochrome photoreceptors are constantly cycling between inactive (oxidized) and activated (reduced) redox states, such that the net biological activity results from the sum of the light-induced (activating) and reverse (de-activating) redox reactions at any given timepoint. A model of the cryptochrome photocycle incorporating these elements and an estimation of the quantum efficiency of redox state interconversions both in vitro and in vivo has been recently derived (Procopio et al. 2016).
Subsequent to their initial discovery in plants, cryptochromes were identified in numerous other organisms including in insects and vertebrates. These homologues are highly similar structurally and some (for instance, insect type I cryptochromes) have been shown to undergo similar redox reactions to plant cryptochromes and activated in a similar manner (Ganguly et al. 2016; Lin et al. 2018).
Of particular interest was the identification of cryptochromes in the UV cones of the avian retina, which are suitably positioned for magnetosensing in migratory birds, which use the geomagnetic field (GMF) for directional orientation (Wiltschko and Wiltschko 2012). The suggestion that cryptochromes may be magnetoreceptors in birds was originally put forward on the grounds that the redox cycle of cryptochromes can generate unpaired radicals, which on theoretical grounds may be affected by weak magnetic fields (MFs; see Hore and Mouritsen 2016 for review). Behavioral studies then showed that wavelengths of light corresponding to the cryptochrome flavin absorption spectrum are in fact correlated with avian magnetoreception, and that, furthermore, the cryptochrome protein in the avian retina undergoes conformational change compatible with the deduced redox cycle of cryptochromes (Nießner et al. 2013; Wiltschko and Wiltschko 2014). Thus, a model for magnetosensing has been suggested, whereby the rate constants (efficiency of redox state interconversion) of the cryptochrome redox cycle are altered in response to weak electromagnetic fields, with a resulting change in biological activity. Support for this suggestion has come from numerous studies in diverse organisms, where cryptochrome mutants are available. Using such mutants, it was possible to show that cryptochromes are required for effects of low-level MFs on growth and developmental responses in Arabidopsis (Ahmad et al. 2007; Xu et al. 2012, 2014, 2015) and on behavioral and physiological responses to low level applied MFs in Drosophila (e.g., Yoshii et al. 2009; Foley et al. 2011; Fedele et al. 2014; Marley et al. 2014; Wu et al. 2016).
The effects of applied MFs on Arabidopsis seedling growth and cryptochrome phosphorylation are relatively minor, and, indeed, require highly controlled conditions of blue-light intensity to visualize. It would, therefore, appear unlikely that Arabidopsis, or, indeed, plants, in general, would show a response to possible minor fluctuations or changes in direction of MFs in the natural environment. Nonetheless, geomagnetic fields (GMFs) have been suggested as a possible factor in plant evolution. During the Tertiary and Cretaceous periods, when Angiosperms diversified into many orders and families, there were also a number of reversals in GMF polarity accompanied by sharp decreases in GMF intensity. The possible influence of these significant changes in GMF has thereby been suggested to be involved in plant evolution (Maffei 2014; Occhipinti et al. 2014; Van der Straeten et al. 2018). Moreover, at significantly elevated MF levels (in the mTessla range), effects on germination and increased root length have been reported, as well as increase in oxidative stress (increased H2O2 levels and decrease in antioxidant activity) (see Maffei 2014 for review). Therefore, a possible role for magnetic sensitivity in plant development and evolution remains an open question.
In order for a biological photoreceptor to function as a magnetoreceptor, it must first produce unpaired radicals with suitable characteristics for interaction with applied MFs, as predicted by the radical-pair hypothesis (Hore and Mouritsen 2016). Cryptochromes are one of the rare classes of photoreceptor that can in fact form multiple such unpaired radicals during activation. They do so at several different steps in the course of flavin redox state interconversion, including both during forward (light-driven) flavin reduction and during reverse (light-independent) flavin reoxidation of the photocycle. As a result, an applied MF could be predicted to alter the chemical rate constants of these redox reactions, creating a difference in the concentration of the activated state receptor to result in altered biological activity.
At the present time, most attention has focussed on the forward (light-driven) electron transfer reaction of cryptochromes as a possible magnetically sensitive reaction. In this reaction, an electron is transferred to the oxidized flavin from the protein surface via a chain of intraprotein electron transfer comprising multiple tryptophan (Trp) and tyrosine (Tyr) residues (see Chaves et al. 2011 for review). This results in the formation of Trp°/FADH° or Tyr°/FADH° radicals which have shown suitable characteristics in modelling studies for interaction with weak MFs and thereby alter the rate constants for redox state interconversion (Hore and Mouritsen 2016). In support of this hypothesis, experimental evidence with isolated Arabidopsis and Drosophila cryptochromes as well as the very similar E. coli photolyase has shown magnetic effects on forward electron transfer reaction rates in vitro, albeit only at relatively high MF strength (in the mT range) (Maeda et al. 2012; Sheppard et al. 2017).
However, a number of recent studies in the avian directional sensing system are in contradiction to this view. First, it has been demonstrated that robins are able to orient to the geomagnetic field (GMF) in green light, at a wavelength that can only be absorbed by the FADH° neutral radical redox state of cryptochrome. In consequence, the flavin radical would be fully reduced in the course of the reaction (from FADH° to FADH-) and there is no possibility for a Trp° or Tyr°/FADH° radical pair to be formed and undergo modification by the MF (Nießner et al. 2013). Even more telling was the result from so-called ‘flicker’ experiments, where birds were subjected to intermittent pulses of light and dark during the behavioral experiments (Wiltschko et al. 2016). In these tests, the birds were exposed to MF direction only during the dark interval between light pulses. The birds in fact proved to be directionally oriented under these conditions. Since a Trp°/flavin or Tyr°/flavin radical pair has a very short lifetime (few milliseconds), these radicals could not be the targets for the MF and, therefore, could not be implicated in magnetosensing. These experiments instead indicated that the magnetosensitive step in the bird orientation experiments must occur in the dark period, which concurs with the reoxidation reaction of cryptochromes.
To help resolve the apparently conflicting lines of reasoning and experimental evidence in the literature, we here present data from Arabidopsis, where sensitivity to applied MFs is mediated through a cryptochrome-dependent mechanism (Ahmad et al. 2007; Xu et al. 2012, 2014, 2015). Using two distinct and unrelated assays for cryptochrome biological activity, we probe the effects of a static MF of 500 μT (10 × earth strength) on Arabidopsis placed under intermittent light/dark conditions. The MF was given solely during the dark interval between light pulses, such that there is a 10 s gap between the cessation of illumination and the onset of the MF. In this way, all transient radical pairs formed during forward electron transfer during the light period are eliminated from consideration as the possible magnetosensing step. Our results indicate that the plants, indeed, show response to the MF applied solely in the dark interval, consistent with data from the avian system (Wiltschko et al. 2016).
Materials and methods
Plant materials and growth conditions
Arabidopsis thaliana wild type (Wassilewskija) and phytochrome A, B deficient mutant lines (Ahmad and Cashmore 1997) were used for cry1 phosphorylation experiments. Transgenic cry1 overexpressing lines as described (Ahmad et al. 2002) were used in hypocotyl growth tests. Seeds were sterilized by incubation with 25% bleach solution for 30 min and subsequently washed 3× with sterile water before plating. Seeds were plated on Petri dishes containing 2% (w/v) sucrose, 0.5X MS Murashige and Skoog Basal Salt Mixture pH 6.0 (MP biomedicals, INC, Illkirch, France), and 0.9% (w/v) agar, and then maintained at 4 °C in the darkness for 48 h. Petri plates were then illuminated with red light (633 nm) for 24 h to induce the germination, and then maintained in darkness for 4 days at 22 °C before the start of the seedling phosphorylation assay. For the seedling growth assay, seedlings were transferred from red light immediately to the test blue light and MF condition for 5 days growth.
Magnetic field (MF) and blue-light exposure conditions
All experiments were performed in an indoor controlled-temperature room (10 m × 2 m × 2 m dimension) at 23 °C with 46 µT of local MF intensity. For the phosphorylation tests, we used a Helmholtz coil to generate a homogeneous 500 µT static MF adjusted parallel to the local GMF. Each coil consisted of 20 windings of 1 mm-diameter copper wire around a square plexiglass frame (20 × 20 × 20 cm diameter) at a separation of 11.5 cm between coils. For the plant seedling growth experiments, two double wound matched Helmholtz coils as described in Ahmad et al. (2007) were used per experiment. The test coil generated a static MF of 500 µT intensity at the plant seedling position. The control field condition was generated by cancelling the MF by currents running in opposite directions in the coils (see Ahmad et al. 2007 for more detailed description). The magnetic intensity in the cancelled field control coil conditions was 40 µT, which was the local GMF.
To provide blue-light exposure, 40 mm-diameter round pre-mounted arrays of 7 high-intensity ‘Royal Blue’ (peak 448 nm) blue-light LEDS (http://www.luxeonstar.com/royal-blue-sinkpad-ii-40mm-7-uP-led-modules) were used together with a circular diffused optic array for optical diffusion of the beam. LEDs were placed 4.5 cm above the seedings at the center of the magnetic coils.
The activation of the magnetic coils and of the LEDs was controlled by custom–built automated programmable switches. These were created using a Raspberry Pi 3B. A 4-channel 5 V power relay board used GPIO pins on the Raspberry Pi to power on both the LED arrays and Helmholz coils. A Scratch program was written to control the GPIO pins connected to the power relay channels and enable the user to create the various light/magnetic field cycle patterns. In addition, a MAX31856 T-type thermocouple board was connected to GPIO pins on the Raspberry Pi and a T-type thermocouple was connected to this board to monitor temperature during the growth process. The MAX31856 board has an open-source python library that was leveraged to record the data from the thermocouple and save it to the raspberry throughout the course of the experiment in real time.
The programmable switch could, therefore, be set to control the current to the LEDs as well as to the MF coils to automatically and continuously generate the alternating pulses of blue light and MF exposure used in this study. Photon fluence of light intensity for the experiment was detected by a Quantum light meter (LI-185B, Li-Cor, Lincoln, NE, USA).
Arabidopsis phyAphyB double mutant etiolated seedlings that had been previously germinated in darkness for 4 days were exposed to the test condition (MF and/or blue-light exposure) as indicated in “Results”. In cases, where the seedlings were exposed to MFs only in the dark interval between light pulses, an interval of 10 s after the end of the light period was inserted before the application of the MF. This resulted in the following programmed exposure condition: (1) 5 min blue light (60 µmol m−2 s−1) at local GMF; (2) 10 s darkness at local GMF; (3) 9 min + 50 s darkness at 500 µT MF. This cycle was repeated six times in succession for this experiment. Subsequently, protein was extracted from plant seedlings and subjected to Western blotting. For the phosphorylation assay, we performed the analysis, as described in Shalitin et al. (2003). The intensity of the upper, phosphorylated band from the Western blotting [cry1(Pi)] was determined using the imaging software and expressed as a percentage of the intensity of the total cry1 protein (sum of phosphorylated plus unphosphorylated cry1) in the same lane. The formula is thereby [cry1(Pi)]/[cry1 (total)] × 100 yielding the percentage of phosphorylated cryptochrome per lane. Three triplicate lanes per individual experiment were averaged to yield the percent phosphorylation at the respective condition (MF or control). The percent phosphorylation between the MF and control conditions was then subtracted to yield the difference in phosphorylation between the test and control (MF vs. control) condition. Thus, a single percentage value of differential phosphorylation was obtained for each biological repeat (e.g., MF vs. control). In sum, five or six independent experiments were performed for each condition (± MF), each involving three replicates.
To obtain the data plotted in Figs. 3, 4, 5, the percentage difference values (MF vs. control) originating from the five or six independent experiments were averaged. The values from these five or six independent experiments were then further subjected to statistical analysis, as described in “Materials and methods” to obtain the P values, as plotted in Figs. 3, 4, 5.
All phosphorylation experiments were analyzed by Western blotting essentially as described (Ahmad et al. 2002; Xu et al. 2014). Triplicate samples of seedings were harvested from each plate per condition and homogenized in SDS-gel electrophoresis sample buffer (2% SDS, 0.5 M Tris–HCl pH 6.8, 20% glycerol, 100 mM β-mercaptoethanol, and 0.01% bromophenol blue), boiled for 10 min. Gel electrophoresis was carried out in a 10% SDS-PAGE separation gel: 30% bis/acrylamide stock (30% stock solution from Sigma), 75 M Tris pH 8.8, 1% SDS, and resolved on a mini-gel apparatus (Amersham, Kingsport, TN, USA) for up to 4 h at 20 mA. Resolved proteins were transferred to a nitrocellulose membrane (Amersham). The efficiency of protein transfer was verified by staining of blots with 2% ponceau S prior to detection by antibody. Anti-cry1 antibody and detection procedure were as described previously (Ahmad et al. 2002; Bouly et al. 2007).
The detected cry1 antibody-specific bands were analyzed by the ImageJ 1.50i software (NIH) to obtain digital values for signal intensities of the slower migrating (phosphorylated) and faster migrating (non-phosphorylated) bands as described previously (Shalitin et al. 2003; Xu et al. 2014). The extent of phosphorylation is expressed as the signal intensities of the slow-migrating bands normalized to the signal intensities of total cryptochrome protein (unphosphorylated plus phosphorylated cry1) in the same gel lane and represented numerically as a ration (cry1(Pi)/cry1). Each experimental sample plate was harvested in triplicate to obtain three separate readings per sample plate per experimental condition. The experimental value per condition was the mean of the three triplicate measurements. For each MF condition, duplicate plates were prepared, such that one plate was used for the test applied MF condition and the other maintained under the cancelled (geomagnetic) field condition, at the same position in the coil and identical illumination. The MF effect is expressed as a percentage of the response to the MF in the test condition in comparison with the response to the local (cancelled) field condition. Each such comparison experiment was repeated for six independent biological repeats performed on different days and using different batches of seeds.
Hypocotyl growth experiment
Subsequent to germination, cry1-overexpressing Arabidopsis seedlings were exposed to the following programmed test condition: (1) 5 min blue light (60 µmol m−2 s−1) at the local GMF; (2) 10 s darkness at local GMF; and (3) 9 min + 50 s of darkness plus 500 µT MF. This exposure regime was repeated continuously over a period of 5 days using the automated switch. Concomitantly with the test condition, in separate coil, a duplicate plate of seedlings was exposed to an identical light regime but with the MF cancelled by opposing currents running through the double wound coils (see Ahmad et al. 2007 for details of cancellation of the field). As a result, the seedlings in the cancelled field condition were never exposed to a 500 µT MF. During the experiment, the temperature was monitored continuously and did not vary significantly between both coils. All experiments were performed double blind, such that the person measuring the seedlings did not know from under which condition they were taken. Hypocotyl lengths of 15 seedlings were measured per plate using the ImageJ 1.50i software (NIH). For each experiment, the mean seedling length in the test condition (+MF) was expressed as a percentage of the mean seedling length of the local field (−MF) condition. Five independent biological repeats were performed under each condition to generate the average value presented in Fig. 3 for statistical analysis.
In addition to the comparison between seedlings with and without exposure to 500 µT MF, we performed a control group experiment (Fig. 1), as for the phosphorylation experiments described above. For the control group, we performed the five replicate biological repeats, using two groups of plates grown under the same Helmholz coils as for the test hypocotyl growth experiments. However, in the control group experiments, none of the seedlings were exposed to an applied MF during the experiment (i.e., compared seedlings exposed to cancelled field to other seedlings exposed to cancelled field). In this way, we obtained the comparison between 2 control-treated groups which were in the identical local GMF condition (40 µT). Since all plates were treated under the identical conditions, the control group would reveal any variability or artifact inherent in our experimental setup or due to inhomogeneous electromagnetic or otherwise conditions in the growth room, where the experiments were conducted.
Data and statistical analysis
As described above, all experiments involved five or six independent biological repeats comparing the effect of application of a 500 µT MF to the identical untreated control seedlings. The MF effect was expressed as a differential percentage (treated vs. untreated sample) for each individual experiment in the MF group. In addition to the test experiments performed in this manner, a control group experiment was performed for each condition. In this case, all plates in a given experiment were treated with the identical light, but with the MF was cancelled. Therefore, no MF was applied to either test or control plates and the effect of the ‘mock’ magnetic field condition expressed as a differential percentage of the ‘local field’ condition (control vs. control sample), which were in fact identical. The data obtained from the MF group and control group were also subjected to the same analysis. The five or six independent values were then subject to statistical analysis (see below) and the average plotted on the graphs ± SE. The schematic of this experimental design is shown in Fig. 1.
For statistical analysis, all data were analyzed by the SPSS Statistics program software IBM SPSS Statistics 23 (IBM). Data were analyzed for normality with the Shapiro–Wilk test (P < 0.05) and homogeneity of variances with Levene (P < 0.05). Results were expressed as mean ± standard error of the mean (SE). The differences between the MF group and the control group were compared using Anova analysis. Differences were considered statistically significant with a P value < 0.05 (*), < 0.01 (**), and < 0.001 (***).
In the prior study, it had been reported that exposure of etiolated Arabidopsis seedlings to a static MF of 500 μT resulted in a significant increase in phosphorylation of Cry1 relative to control seedlings (Xu et al. 2014). We, therefore, began replicating the conditions of this prior study using wild-type seedlings of ecotype Wassilewskija. In our experiments, we illuminated the wild-type seedlings at 60 μmol m−2 s−1 blue light for 30 min in the absence (control) or the presence (MF) of a 500 μT applied staticMF, which is roughly tenfold the earth’s GMF. In our initial experiment, we obtained a measurable increase in phosphorylation due to application of the MF (Fig. 2b), consistent with the prior report (Xu et al. 2014).
We next attempted to improve the sensitivity of the assay, by testing the effects of applied MFs on seedlings that are deficient in the red/far red receptor phytochrome. Phytochrome is known to interact with cryptochrome at many levels and enhances and maintains plant downstream responses to blue light (Ahmad and Cashmore 1997; Mazzella et al. 2001). These interactions, furthermore, include a direct phosphorylation of the C-terminal domain of cryptochrome by phytochrome A, which likely also occurs in vivo (Ahmad et al. 1998). Therefore, phytochrome action could mask and reduce any visible effect of an externally applied MF on cryptochrome responses, as phytochromes do not form radical pairs required for chemical magnetosensing. Indeed, we found that the phyAphyB deficient mutants showed a visibly enhanced response to an applied MF in our preliminary experiments (Fig. 2b). For all subsequent phosphorylation experiments, we, therefore, used the phyAphy mutant seedling lines.
Cryptochrome undergoes a photocycle, whereby flavin is reduced in response to light. The extent of flavin reduction is determined by the light intensity at quantum efficiencies that have been calculated empirically from in vitro and in vivo experiments (Procopio et al. 2016). Therefore, for magnetic sensitivity to occur, the light intensity should be at an intensity that is below saturation, but sufficiently above threshold for small changes in cryptochrome sensitivity to be readily visible in the phenotypic response. To assess the optimum illumination conditions for observing magnetic sensitivity, we accordingly performed phosphorylation experiments at increasing blue-light intensities ranging from 10 to 120 μmol m−2 s−1. Phosphorylation under the applied MF condition was expressed as a percentage of the phosphorylation obtained under identical illumination conditions in the absence of the applied field. Six duplicate experiments were performed for each condition, involving independent biological repeats (see “Materials and methods”). Temperature was measured by a thermocouple attached to a computer and monitored throughout the experiment.
The MF sensitivity was expressed as the percent increase in phosphorylation in the presence of an MF as compared to that of seedlings in the control condition. Statistical analysis was performed on six independently performed experiments to obtain statistical P values (see “Materials and methods”).
There are many hidden variables that may affect perception of MFs in plants and other organisms, which has made such studies historically difficult to replicate. For example, the existence of high concentrations of metal in the building, or of random electromagnetic noise or of electronic background signals can apparently perturb avian directional sensing which is presumed to follow from a cryptochrome-dependent mechanism (see, e.g., Engels et al. 2014). We, therefore, devised an additional control experiment to eliminate all possible artifacts due to the experimental setup or the environment in the room, where the experiments were performed. In this additional ‘control’ experiment, we performed six independent biological repeats of the illumination treatments exactly as for the MF test experiment. However, in the control experiment, none of the plates had received an applied MF, and therefore, in this experiment, we measured the percentage phosphorylation differences between each of six ‘mock’-treated plates to each of six untreated plates. In this way, the inherent variation in our experimental setup could be unequivocally determined. Further details of the experimental design and procedures, image analysis, and statistical treatments are found in “Materials and methods”.
The data presented in Fig. 3b represent the average of all of the six independent biological repeats, comparing the increase in phosphorylation after exposure to a 500 μT MF (MF, grey squares) at the indicated blue-light intensities. For the control experiments (control, white squares), six independent experiments were performed comparing phosphorylation in non-exposed seedlings to ‘mock’ exposed seedlings, such that neither sample group had been exposed to an MF (see “Materials and methods”).
As expected, there was no significant variation in phosphorylation in the control samples, which consisted of repetitions under identical conditions of 6 independent biological repeats of pairs of samples receiving the same exposure to the local MF. The control sample thereby represents the inherent variation of the experimental method used for each condition. By contrast, under 30, 60, and 120 μmol m−2 s−1 blue-light illumination, there was a significant increase in phosphorylation as a result of exposure to the MF. The maximal effect was about 12% increased phosphorylation in seedlings exposed to the MF as compared to control seedlings (P value < 0.01) at 60 μmol m−2 s−1, which occurs well below saturation in fluence response range deduced for Cry1 (Procopio et al. 2016).
To date, two distinct steps in the cryptochrome redox cycle have come under consideration as possible points of interaction with an MF. The first occurs during forward (light-driven) electron transfer, where electrons are transferred from the protein surface to the flavin via a chain of intraprotein electron transfer involving Trp° and Tyr° radicals. In this case, the radical pairs are short-lived intermediates with a maximum lifetime of less than 100 ms (Thöing et al. 2015; Nohr et al. 2016) in the isolated proteins. By contrast, evidence from the avian system has shown that magnetic sensitivity can occur in darkness if MF pulses are given in the dark periods between pulses of illumination (Wiltschko et al. 2016). In this way, short-lived radical pairs such as formed during forward electron transfer are eliminated from consideration as possible targets for interaction with MFs, as they decay too rapidly to be present when the MF is applied in the dark intervals.
Next, we determined whether the response to the MF could only occur when the MF was applied at the same time as the illumination. For this experiments, we use the 5 min blue light/10 min dark repeated pulsed light condition as described above; however, we applied the static field only during the 10 min dark intervals between the 5 min light pulses. To ensure that no radicals formed under conditions of illumination were carried over into the subsequent dark period, we provided a 10 s gap in darkness after the end of the illumination period and the onset of the MF. The results showed that there was a 19% increase in phosphorylation of cry1 under this regime (P value < 0.001; Fig. 4). These results show that the MF effect on cryptochrome occurs during the process of the return of the receptor to its resting state, corresponding to the period of flavin reoxidation in the cryptochorme photocycle (Müller and Ahmad 2011).
As an additional and complementary test for cryptochrome response to MFs, we used the hypocotyl growth inhibition assay, which is a cryptochrome-dependent response under blue light which has been previously shown to be modulated by an applied MF (Ahmad et al. 2007). We have here modified and optimized this assay as compared to prior studies (Ahmad et al. 2007) using transgenic seedlings which overexpress the Cry1 receptor protein. In these lines, cryptochrome protein expression is enhanced five times over background levels leading to correspondingly enhanced cryptochrome function (Ahmad et al. 2002). In addition, we have conducted the growth experiments over 5 days instead of 72 h as in prior studies, and used the illumination conditions which showed the optimum response to the applied MF for the phosphorylation experiments (from Fig. 4).
To eliminate the possibility of artifact ensued from unknown electromagnetic background in the test chamber, and to get a baseline value for the inherent variability in our experimental setup, we again performed the entire experiment using the identical magnetic coils and illumination conditions, in the absence of an MF. That is, both plates of a matched set of seedlings were grown under identical illumination conditions, at the identical positions in the Helmholz coils as the MF test experiments, and both receiving the identical mock treatments (antiparallel cancelled fields). In other words, all seedlings in the test experiment received the same, earth strength MF. As expected, the results of this control experiment showed a variability of only ± 2.5%, with no statistical significance in seedling length between the two groups of seedlings under the two coils.
Therefore, the effect of the applied MF under the test condition is conclusive and provides evidence that the effect of the static MF on the cryptochrome receptor occurs at a step on the reaction pathway that is independent of light.
The suggestion that a biological response to weak (near earth strength) MFs can be mediated by a radical-pair (RP) mechanism was first proposed by Schulten et al. (1976) and has gained wide spread acceptance in the past 30 years. In particular, a broad literature has shown experimentally that the rate, yield, and/or product distribution of chemical reactions which involve radical intermediates can, indeed, be modified by MFs (Rodgers 2009). For biological responses, cryptochrome receptors had been initially proposed as possible magnetosensors by Ritz et al. (2000) and subsequently implicated experimentally in MF sensitivity in organisms ranging from plants to fruit flies and migratory birds (Ritz et al. 2010; Hore and Mouritsen 2016). The current challenge is to determine the underlying chemistry of how the MF can interact with this biological sensor, and in particular to identify the relevant radical-pair intermediates and reaction rates that are altered.
Up until now, attention has been focussed primarily on the forward electron transfer reaction of cryptochromes, whereby excited state flavin is reduced through a chain of intramolecular electron transfer involving Trp° and Tyr° radicals. The resulting Trp°/FADH° or Trp°/FAD-° radicals are presumed targets for electromagnetic fields, which as a consequence alter the rate of flavin photoreduction which leads to biological activation (see Ahmad 2016; Ganguly et al. 2016; Lin et al. 2018) for current discussion of the role of redox state interconversion in cryptochrome photo-sensing). This reaction has been deemed particularly suitable for magnetic sensitivity on theoretical grounds (see, e.g., Mouritsen and Hore 2012; Dodson et al. 2013; Lüdemann et al. 2015; Hong and Pachter 2015; Worster et al. 2016). It has also been shown experimentally that applied MFs can alter flavin reduction rates using isolated cryptochrome proteins from plants and Drosophila, albeit at high (mT) MF intensities (Maeda et al. 2012; Sheppard et al. 2017). However, recent evidence from the avian system has shown that the Trp°/FADH° radical pair cannot be involved in magnetosensing, as the birds are oriented in green light during which these radicals are not formed (Nießner et al. 2013). Furthermore, it has been shown that birds subjected to alternating pulses of light and darkness show directional magnetic sensitivity even when the MF is applied solely during the dark intervals between light pulses (Wiltschko et al. 2016). These results are inconsistent with forward electron transfer to the flavin as the reaction step at which the MF can act.
In this study, we have examined the magnetic sensitivity of Arabidopsis cryptochrome in light of the known parameters of the cryptochrome photocycle (Procopio et al. 2016). To ensure reproducibility of our methods, we have replicated a reported direct assay from another lab for MF sensitivity of cryptochrome photoreceptor phosphorylation (Xu et al. 2014). This is a rapid assay (30–90 min duration) that measures changes in conformational state at the level of the photoreceptor molecule itself. It results from C-terminal conformational changes which occur upon cryptochrome activation and which render phosphorylation sites accessible to cellular kinases (Tan et al. 2013; Wang et al. 2015). Phosphorylation only occurs in response to blue light, as it is a direct consequence of cryptochrome photoreceptor activation (Tan et al. 2013; Wang et al. 2015). However, continuous light is not necessary for biological activity as the cryptochrome active (photoreduced) redox state is stable for a period of several min even after return of the system to darkness (Bouly et al. 2007; Herbel et al. 2013; Procopio et al. 2016). Therefore, robust cryptochrome activation, which includes phosphorylation, can be observed under pulsed light conditions, wherein brief successive periods of illumination are alternated with periods of complete darkness.
Under continuous illumination, using the phosphorylation assay, we observed an increase in cryptochrome phosphorylation in response to an applied MF relative to control seedlings maintained under the same illumination conditions but without applied field. This indicated that the applied 500 μT field enhanced the cryptochrome biological response (extent of receptor activation) to illumination, consistent with reports from the prior study using this method (Xu et al. 2014). Using pulsed light conditions of 5 min light alternating with 10 min of dark, we also observed cryptochrome sensitivity to an applied MF, in this case even more pronounced than under continuous illumination. Significantly, applying the MF solely in the dark intervals between blue-light illumination pulses resulted in the same stimulation of protein phosphorylation as found when the MF was applied during both light and dark intervals (continuously). This indicates that the MF cannot act at a reaction step in the cryptochrome photocycle that involves the forward electron transfer pathway, as these radical-pair intermediates (Trp°/FADH° or Tyr°/FADH°) have reported lifetimes only in the millisecond time scale (see, e.g., Thöing et al. 2015).
These results were further confirmed by use of an independent assay for cryptochrome photoreceptor function, namely, inhibition of hypocotyl elongation in Arabidopsis seedlings. In this assay, seedling growth is inhibited in response to blue light through activation of cryptochrome, such that the greater the cryptochrome biological activity, the shorter the seedling hypocotyl length in response to blue light. Application of weak MFs has been reported to alter hypocotyl growth inhibition in the past studies (Ahmad et al. 2007; Xu et al. 2012), although other labs have reported difficulty in reproducing these results (Harris et al. 2009).
We believe that the reasons for variable results among different labs attempting the hypocotyl growth inhibition protocol are due to the already weak nature of the reported biological response and the many cellular factors (including the action of hormones, phytochromes, and other metabolic processes) that contribute to elongation growth and thereby mask the effect of cryptochromes (see, e.g., Cheng et al. 2013; Hu et al. 2017). Furthermore, different lab environments may have differing amounts of metal or electronic equipment generating background electromagnetic noise (Engels et al. 2014), and also there could be other forms of interference with the relatively weak MFs used to elicit differential plant growth responses. Therefore, to ensure reproducibility of this method under variable growth conditions in different labs, we have optimized the hypocotyl growth assay in the current study using cryptochrome overexpressing seedlings. These seedlings have approximately fivefold increase in cryptochrome with respect to wild-type seedlings, such that the cryptochrome-dependent signal effectively dominates the growth inhibition response in the expressing plant and reduces the interference from additional growth factors (Ahmad et al. 2002). We have also further increased the period of seedling growth from 3 to 5 days, to maximize the effect of differential growth under the applied MF, and used the optimized illumination protocols developed in the current study. Using this optimized hypocotyl growth inhibition protocol, we observed approximately 20% reduced seedling growth upon application of an applied MF (Fig. 5). Moreover, this response was obtained even when the MF was applied exclusively in the dark interval between successive light pulses (Fig. 5).
At present, theoretical considerations have argued against a flavin/superoxide radical pair, which is formed in the course of flavin reoxidation (Müller and Ahmad 2011), as the magnetosensing intermediate in cryptochromes (reviewed in Hore and Mouritsen 2016). However, cryptochrome localized within living cells is in contact with many cellular metabolites, which, moreover, can move into the flavin pocket in close association with the flavin cofactor (see, e.g., Engelhard et al. 2014). Therefore, the possibility of the third party cellular factors participating in the formation of radical pairs during the process of cryptochrome flavin reoxidation cannot be excluded. Finally, there is as yet no conclusive evidence that cryptochrome is, in fact, a magnetosensor. Indeed, there have been reports of magnetosensing, whereby cryptochromes interact with an iron-based magnetosensor MagR (Qin et al. 2016) as opposed to the current radical-pair-based (chemical) mechanism (Hore and Mouritsen 2016) discussed here. Future theoretical studies taking all of these possibilities into account, such as properties of potential scavenging intermediates in magnetic sensitivity (Kattnig and Hore 2017), are, therefore, required to provide the necessary framework for further experimental work to identify the actual magnetosensing intermediate states.
MA designed the experiments, MA, CFM, JL, and MP wrote the paper; MP and LDA conducted experiments, DB, CFM, JL built equipment and designed analytical tools, MA and MP analyzed the data, and all authors read and approved the manuscript.
We are indebted to Adam Purvis, Dorothy Engle and Haider Raad for valuable technical assistance and support at many stages of the project. Support was from NSF (#1658640), AFOSR (FA9550-14-0-0409) and AFOSR (FA9550-17-1-0458).
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