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Genome-wide identification and characterization of ALTERNATIVE OXIDASE genes and their response under abiotic stresses in Camellia sinensis (L.) O. Kuntze

  • Chang-Qing Ding
  • Sophia Ng
  • Lu Wang
  • Yu-Chun Wang
  • Na-Na Li
  • Xin-Yuan Hao
  • Jian-Ming Zeng
  • Xin-Chao Wang
  • Ya-Jun Yang
Original Article

Abstract

Main conclusion

Four typical ALTERNATIVE OXIDASE genes have been identified in tea plants, and their sequence features and gene expression profiles have provided useful information for further studies on function and regulation.

Abstract

Alternative oxidase (AOX) is a terminal oxidase located in the respiratory electron transport chain. AOX catalyzes the oxidation of quinol and the reduction of oxygen into water. In this study, a genome-wide search and subsequent DNA cloning were performed to identify and characterize AOX genes in tea plant (Camellia sinensis (L.) O. Kuntze cv. Longjing43). Our results showed that tea plant possesses four AOX genes, i.e., CsAOX1a, CsAOX1d, CsAOX2a and CsAOX2b. Gene structure and protein sequence analyses revealed that all CsAOXs share a four-exon/three-intron structure with highly conserved regions and amino acid residues, which are necessary for AOX secondary structures, catalytic activities and post-translational regulations. All CsAOX were shown to localize in mitochondria using the green fluorescent protein (GFP)-targeting assay. Both CsAOX1a and CsAOX1d were induced by cold, salt and drought stresses, and with different expression patterns in young and mature leaves. Reactive oxygen species (ROS) accumulated strongly after 72 and 96 h cold treatments in both young and mature leaves, while the polyphenol and total catechin decreased significantly only in mature leaves. In comparison to AtAOX1a in Arabidopsis thaliana, CsAOX1a lost almost all of the stress-responsive cis-acting regulatory elements in its promoter region (1500 bp upstream), but possesses a flavonoid biosynthesis-related MBSII cis-acting regulatory element. These results suggest a link between CsAOX1a function and the metabolism of some secondary metabolites in tea plant. Our studies provide a basis for the further elucidation of the biological function and regulation of the AOX pathway in tea plants.

Keywords

CsAOX Cold stress Flavonoid biosynthesis Reactive oxygen species Secondary metabolisms Tea plant 

Abbreviations

AOX

Alternative oxidase

ROS

Reactive oxygen species

Notes

Acknowledgements

This work was supported by the Central Public-interest Scientific Institution Basal Research Fund (1610212017003), the Earmarked Fund for China Agriculture Research System (CARS-19) and the Major Project of Agricultural Science and Technology in Breeding of Tea Plant Variety in Zhejiang Province (2016C02053-4). SN is supported by the European Molecular Biology Organization (EMBO) Long–term Fellowship (ALTF1239-2015) and Marie Curie Actions, which are funded by the European Commission (LTFCOFUND2013, GA-2013-609409).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interest.

Supplementary material

425_2018_2974_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)
425_2018_2974_MOESM2_ESM.docx (14 kb)
Supplementary material 2 (DOCX 14 kb)

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Copyright information

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Authors and Affiliations

  1. 1.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, National Center for Tea ImprovementTea Research Institute, Chinese Academy of Agricultural SciencesHangzhouPeople’s Republic of China
  2. 2.ARC Centre of Excellence Plant Energy BiologyThe University of Western AustraliaCrawleyAustralia
  3. 3.Institut de Biosciences et BiotechnologiesCommissariat à l’Énergie Atomique et aux Énergies Alternatives, CadaracheSt Paul-Lez-DuranceFrance

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