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Planta

, Volume 248, Issue 4, pp 893–907 | Cite as

LeSPL-CNR negatively regulates Cd acquisition through repressing nitrate reductase-mediated nitric oxide production in tomato

  • Wei Wei Chen
  • Jian Feng Jin
  • He Qiang Lou
  • Li Liu
  • Leon V. Kochian
  • Jian Li YangEmail author
Original Article

Abstract

Main conclusion

An SPL-type transcription factor, LeSPL-CNR, is negatively involved in NO production by modulating SlNR expression and nitrate reductase activity, which contributes to Cd tolerance.

Cadmium (Cd) is a highly toxic pollutant. Identifying factors affecting Cd accumulation in plants is a prerequisite for minimizing dietary uptake of Cd from crops grown with contaminated soil. Here, we report the involvement of a SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor LeSPL-CNR in Cd tolerance in tomato (Solanum lycopersicum). In comparison with the wild-type Ailsa Craig (AC) plants, the Colourless non-ripening (Cnr) epimutant displayed increased Cd accumulation and enhanced sensitivity to Cd, which was in well accordance with the repression of LeSPL-CNR expression. Cd stress-induced NO production was inhibited by nitrate reductase (NR) inhibitor, but not NO synthase-like enzyme inhibitor. Expression of LeSPL-CNR was negatively correlated with SlNR expression and the NR activity. We also demonstrated that LeSPL-CNR inhibited the SlNR promoter activity in vivo and bound to SlNR promoter sequence that does not contain a known SBP-binding motif. In addition, expression of an IRON-REGULATED TRANSPORTER1, SlIRT1, was more abundant in Cnr roots than AC roots under Cd stress. LeSPL-CNR may thus provide a molecular mechanism linking Cd stress response to regulation of NR-dependent NO production, which then contributes to Cd uptake via SlIRT1 expression in tomato.

Keywords

Cadmium toxicity Iron uptake Solanum lycopersicum Transcription factor 

Abbreviations

AC

Ailsa Craig

Cnr

Colourless non-ripening

DAF-FM DA

4-Amino-5methylamino-2′,7′-difluorescein diacetate

IRT1

IRON-REGULATED TRANSPORTER1

NO

Nitric oxide

NOS

Nitric oxide synthase

NR

Nitrate reductase

SlNR

Solanum lycopersicum nitrate reductase

SPL

SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE

TF

Transcription factor

Notes

Acknowledgements

This work was supported financially by the Natural Science Foundation of China (31222049) and The Chang Jiang Scholars Program (JLY). We are grateful to Prof. Yiguo Hong (Hangzhou Normal University) for providing us the tomato seeds and his critical comments to the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

425_2018_2949_MOESM1_ESM.docx (2 mb)
Supplementary material 1 (DOCX 2020 kb)
425_2018_2949_MOESM2_ESM.xlsx (12 kb)
Supplementary material 2 (XLSX 12 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Research Centre for Plant RNA Signaling, College of Life and Environmental SciencesHangzhou Normal UniversityHangzhouChina
  2. 2.State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouChina
  3. 3.State Key Laboratory of Subtropical Silviculture, School of Forestry and BiotechnologyZhejiang A&F UniversityLin’anChina
  4. 4.Global Institute for Food SecurityUniversity of SaskatchewanSaskatoonCanada

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