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Planta

, Volume 248, Issue 2, pp 513–518 | Cite as

The HAF2 protein shapes histone acetylation levels of PRR5 and LUX loci in Arabidopsis

Short Communication

Abstract

Main conclusion

The histone acetyltransferase HAF2 facilitates H3 acetylation deposition at the PRR5 and LUX promoters to contribute to robust circadian oscillation.

The circadian clock ensures synchronization of endogenous rhythmic processes with environmental cycles. Multi-layered regulation underlies precise circadian oscillation, and epigenetic regulation is emerging as a crucial scheme for robust circadian maintenance. Here, we report that HISTONE ACETYLTRANSFERASE OF THE TAFII250 FAMILY 2 (HAF2) is involved in circadian homeostasis. The HAF2 gene is activated at midday, and its temporal expression is shaped by CIRCADIAN CLOCK-ASSOCIATED 1. The midday-activated HAF2 protein stimulates H3 acetylation (H3ac) deposition at the PRR5 and LUX loci, contributing to establishment of the raising phase. These results indicate that epigenetic waves in circadian networks underlie temporal compartmentalization of circadian components and stable maintenance of circadian oscillation.

Keywords

Circadian clock Epigenetic modification HAF2 Histone acetylation 

Abbreviations

CCA1

CIRCADIAN CLOCK-ASSOCIATED 1

ChIP

Chromatin immunoprecipitation

H3ac

Histone 3 acetylation

HAF2

HISTONE ACETYLTRANSFERASE OF THE TAFII250 FAMILY 2

LHY

LATE ELONGATED HYPOCOTYL

LUX

LUX ARRHYTHMO

PRR

PSEUDO-RESPONSE REGULATOR

ZT

Zeitgeber time

Notes

Acknowledgements

This work was supported by the Basic Science Research (NRF-2016R1D1A1B03931139) and Basic Research Laboratory (NRF-2017R1A4A1015620) programs provided by the National Research Foundation of Korea and by the Next-Generation BioGreen 21 Program (PJ01314501) provided by the Rural Development Administration.

Supplementary material

425_2018_2921_MOESM1_ESM.pdf (253 kb)
Supplementary material 1 (PDF 252 kb)

References

  1. Alabadi D, Oyama T, Yanovsky MJ, Harmon FG, Mas P, Kay SA (2001) Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock. Science 293(5531):880–883.  https://doi.org/10.1126/science.1061320 CrossRefPubMedGoogle Scholar
  2. Benhamed M, Bertrand C, Servet C, Zhou DX (2006) Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression. Plant Cell 18(11):2893–2903.  https://doi.org/10.1105/tpc.106.043489 CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bertrand C, Benhamed M, Li YF, Ayadi M, Lemonnier G, Renou JP, Delarue M, Zhou DX (2005) Arabidopsis HAF2 gene encoding TATA-binding protein (TBP)-associated factor TAF1, is required to integrate light signals to regulate gene expression and growth. J Biol Chem 280(2):1465–1473.  https://doi.org/10.1074/jbc.M409000200 CrossRefPubMedGoogle Scholar
  4. Green RM, Tobin EM (2002) The role of CCA1 and LHY in the plant circadian clock. Dev Cell 2(5):516–518CrossRefPubMedGoogle Scholar
  5. Hemmes H, Henriques R, Jang IC, Kim S, Chua NH (2012) Circadian clock regulates dynamic chromatin modifications associated with Arabidopsis CCA1/LHY and TOC1 transcriptional rhythms. Plant Cell Physiol 53(12):2016–2029.  https://doi.org/10.1093/pcp/pcs148 CrossRefPubMedPubMedCentralGoogle Scholar
  6. Lee HG, Lee K, Jang K, Seo PJ (2015) Circadian expression profiles of chromatin remodeling factor genes in Arabidopsis. J Plant Res 128(1):187–199.  https://doi.org/10.1007/s10265-014-0665-8 CrossRefPubMedGoogle Scholar
  7. Lu SX, Knowles SM, Webb CJ, Celaya RB, Cha C, Siu JP, Tobin EM (2011) The Jumonji C domain-containing protein JMJ30 regulates period length in the Arabidopsis circadian clock. Plant Physiol 155(2):906–915.  https://doi.org/10.1104/pp.110.167015 CrossRefPubMedGoogle Scholar
  8. Malapeira J, Khaitova LC, Mas P (2012) Ordered changes in histone modifications at the core of the Arabidopsis circadian clock. Proc Natl Acad Sci USA 109(52):21540–21545.  https://doi.org/10.1073/pnas.1217022110 CrossRefPubMedGoogle Scholar
  9. Nakamichi N (2011) Molecular mechanisms underlying the Arabidopsis circadian clock. Plant Cell Physiol 52(10):1709–1718.  https://doi.org/10.1093/pcp/pcr118 CrossRefPubMedPubMedCentralGoogle Scholar
  10. Nusinow DA, Helfer A, Hamilton EE, King JJ, Imaizumi T, Schultz TF, Farre EM, Kay SA (2011) The ELF4-ELF3-LUX complex links the circadian clock to diurnal control of hypocotyl growth. Nature 475(7356):398–402.  https://doi.org/10.1038/nature10182 CrossRefPubMedPubMedCentralGoogle Scholar
  11. Seo PJ, Mas P (2014) Multiple layers of posttranslational regulation refine circadian clock activity in Arabidopsis. Plant Cell 26(1):79–87.  https://doi.org/10.1105/tpc.113.119842 CrossRefPubMedPubMedCentralGoogle Scholar
  12. Seo PJ, Mas P (2015) STRESSing the role of the plant circadian clock. Trends Plant Sci 20(4):230–237.  https://doi.org/10.1016/j.tplants.2015.01.001 CrossRefPubMedGoogle Scholar
  13. Wang L, Kim J, Somers DE (2013) Transcriptional corepressor TOPLESS complexes with pseudoresponse regulator proteins and histone deacetylases to regulate circadian transcription. Proc Natl Acad Sci USA 110(2):761–766.  https://doi.org/10.1073/pnas.1215010110 CrossRefPubMedGoogle Scholar
  14. Yakir E, Hilman D, Kron I, Hassidim M, Melamed-Book N, Green RM (2009) Posttranslational regulation of CIRCADIAN CLOCK ASSOCIATED 1 in the circadian oscillator of Arabidopsis. Plant Physiol 150(2):844–857.  https://doi.org/10.1104/pp.109.137414 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Zhang C, Xie Q, Anderson RG, Ng G, Seitz NC, Peterson T, McClung CR, McDowell JM, Kong D, Kwak JM, Lu H (2013) Crosstalk between the circadian clock and innate immunity in Arabidopsis. PLoS Pathog 9(6):e1003370.  https://doi.org/10.1371/journal.ppat.1003370 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological SciencesSungkyunkwan UniversitySuwonRepublic of Korea

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