, Volume 249, Issue 4, pp 1177–1188 | Cite as

The apple U-box E3 ubiquitin ligase MdPUB29 contributes to activate plant immune response to the fungal pathogen Botryosphaeria dothidea

  • Peng-Liang Han
  • Yuan-Hua Dong
  • Kai-Di Gu
  • Jian-Qiang Yu
  • Da-Gang HuEmail author
  • Yu-Jin HaoEmail author
Original Article


Main conclusion

MdPUB29 is a positive regulator of the defense response to the fungal pathogen Botryosphaeria dothidea possibly by directly regulating the salicylic acid (SA) content as well as SA synthesis-related and signaling-related gene transcription.

In plants, ubiquitin E3 ligases containing a U-box domain (PUBs, Plant U-box E3 ubiquitin ligase) have been identified as key regulators of fundamental cellular processes, such as cellular growth, development, and apoptosis, as well as biotic and abiotic stress responses. However, the function of PUBs in apple ring rot remains elusive. Here, we isolated the U-box E3 ligase MdPUB29 from the apple cultivar ‘Royal Gala’ and characterized its function in plant pathogen defense against Botryosphaeria dothidea. qRT-PCR showed that the expression of MdPUB29 was significantly induced in apple fruits after B. dothidea infection. Overexpression of the MdPUB29 gene in apple calli increased the resistance to B. dothidea infection. In contrast, silencing MdPUB29 in apple calli resulted in reduced resistance. Ectopic expression of MdPUB29 in Arabidopsis also exhibited enhanced resistance to B. dothidea infection compared to that of the wild-type (Col) control. In addition, it was found that the increase of plant pathogen defense was correlated with the increased salicylic acid (SA) content, as well as SA synthesis-related and signaling-related gene transcription in comparison to the wild type. We elucidated the mechanism by which MdPUB29 elevates plant pathogen defense against B. dothidea possibly by regulating the SA pathway.


Apple Botryosphaeria dothidea Callose deposition E3 ubiquitin ligase MdPUB29 Pathogen resistance ROS Salicylic acid 



PAMPs-triggered immunity


Plant U-box E3 ubiquitin ligase


Reactive oxygen species


Salicylic acid



We would like to thank Prof. Takaya Moriguchi of National Institute of Fruit Tree Science, Japan, for ‘Orin’ apple calli. We also would like to thank TopEdit English Editing LLC for providing linguistic assistance during the preparation of this manuscript. This work was supported by Grants from the National Natural Science Foundation of China (31601728, 31471854 and 31772288), Ministry of Education of China (IRT15R42), Shandong Province (ZR2016CQ13 and SDAIT-06-03), and Shandong Agricultural University (564024, 24024).

Supplementary material

425_2018_3069_MOESM1_ESM.pdf (113 kb)
Supplementary material 1 (PDF 113 kb)
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Supplementary material 2 (PDF 236 kb)
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Supplementary material 3 (PDF 233 kb)
425_2018_3069_MOESM4_ESM.pdf (159 kb)
Supplementary material 4 (PDF 159 kb)
425_2018_3069_MOESM5_ESM.pdf (76 kb)
Supplementary material 5 (PDF 75 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and EngineeringShandong Agricultural UniversityTai’anChina

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