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Planta

, Volume 247, Issue 4, pp 875–886 | Cite as

The microtubule-associated RING finger protein 1 (OsMAR1) acts as a negative regulator for salt-stress response through the regulation of OCPI2 (O. sativa chymotrypsin protease inhibitor 2)

  • Yong Chan Park
  • Sandeep Chapagain
  • Cheol Seong JangEmail author
Original Article

Abstract

Main conclusion

Our results suggest that a rice E3 ligase, OsMAR1, physically interacts with a cytosolic protein OCPI2 and may play an important role under salinity stress.

Salt is an important abiotic stressor that negatively affects plant growth phases and alters development. Herein, we found that a rice gene, OsMAR1 (Oryza sativa microtubule-associated RING finger protein 1), encoding the RING E3 ligase was highly expressed in response to high salinity, water deficit, and ABA treatment. Fluorescence signals of its recombinant proteins were clearly associated with the microtubules in rice protoplasts. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) showed that OsMAR1 interacted with a cytosolic protein OCPI2 (O. sativa chymotrypsin protease inhibitor 2) and led to its degradation via the 26S proteasome. Heterogeneous overexpression of OsMAR1 in Arabidopsis showed retarded root growth compared with that of control plants, and then led to hypersensitivity phenotypes under high salinity stress. Taken together, OsMAR1 negatively regulates the salt-stress response via the regulation of the OCPI2 protein in rice.

Keywords

Microtubule Heterogeneous overexpression OsMAR1 OCPI2 Rice RING finger protein Salt stress 

Abbreviations

OCPI2

Oryza sativa chymotrypsin protease inhibitor 2

OsMAR1

Oryza sativa microtubule-associated RING finger protein 1

Ub

Ubiquitin

Notes

Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2016R1A2B4015626).

Supplementary material

425_2017_2834_MOESM1_ESM.pdf (42 kb)
Supplementary material 1 (PDF 42 kb)
425_2017_2834_MOESM2_ESM.pdf (42 kb)
Supplementary material 2 (PDF 41 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Plant Genomics Lab, Department of Applied Plant SciencesKangwon National UniversityChuncheonRepublic of Korea

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