Impact of two arbuscular mycorrhizal fungi on Arundo donax L. response to salt stress
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AM symbiosis did not strongly affect Arundo donax performances under salt stress, although differences in the plants inoculated with two different fungi were recorded.
The mechanisms at the basis of the improved tolerance to abiotic stresses by arbuscular mycorrhizal (AM) fungi have been investigated mainly focusing on food crops. In this work, the potential impact of AM symbiosis on the performance of a bioenergy crop, Arundo donax, under saline conditions was considered. Specifically, we tried to understand whether AM symbiosis helps this fast-growing plant, often widespread in marginal soils, withstand salt. A combined approach, involving eco-physiological, morphometric and biochemical measurements, was used and the effects of two different AM fungal species (Funneliformis mosseae and Rhizophagus irregularis) were compared. Results indicate that potted A. donax plants do not suffer permanent damage induced by salt stress, but photosynthesis and growth are considerably reduced. Since A. donax is a high-yield biomass crop, reduction of biomass might be a serious agronomical problem in saline conditions. At least under the presently experienced growth conditions, and plant–AM combinations, the negative effect of salt on plant performance was not rescued by AM fungal colonization. However, some changes in plant metabolisms were observed following AM-inoculation, including a significant increase in proline accumulation and a trend toward higher isoprene emission and higher H2O2, especially in plants colonized by R. irregularis. This suggests that AM fungal symbiosis influences plant metabolism, and plant–AM fungus combination is an important factor for improving plant performance and productivity, in presence or absence of stress conditions.
KeywordsAM symbiosis Bioenergy crop Climate change Giant reed Plant tolerance Salinity
Intrinsic water use efficiency
This work was funded by Progetto Premiale 2012 CNR-Biofuels and third-generation biorefinery integrated with the territory. The authors thank Maria Teresa della Beffa for the help during plant preparation and growth.
- Antoniou C, Filippou P, Mylona P, Fasoula D, Ioannides I, Polidoros A, Fotopoulos V (2013) Developmental stage- and concentration-specific sodium nitroprusside application results in nitrate reductase regulation and the modification of nitrate metabolism in leaves of Medicago truncatula plants. Plant Sign Behav 8:9CrossRefGoogle Scholar
- Armada E, Azcón R, López-Castillo OM, Calvo Polanco M, Ruiz-Lozano JM (2015) Autochthonous arbuscular mycorrhizal fungi and Bacillus thuringiensis from a degraded Mediterranean area can be used to improve physiological traits and performance of a plant of agronomic interest under drought conditions. Plant Physiol Biochem 90:64–74CrossRefPubMedGoogle Scholar
- Balestrini R, Chitarra W, Fotopoulos V, Ruocco M (2017a) Potential role of beneficial soil microorganisms in plant tolerance to abiotic stress. In: Lukac M, Gamboni M, Grenni P (eds) Soil biological communities and ecosystem resilience. Sustainability in plant and crop protection. Springer, New York, pp 269–283. ISBN 978-3-319-63335-0Google Scholar
- Baraza E, Tauler M, Romero-Munar A, Cifre J, Gulias J (2016) Mycorrhiza-based biofertilizer application to improve the quality of Arundo donax L. plantlets. In: Barth S, Murphy-Bokern D, Kalinina O, Taylor G, Jones M (eds) Perennial biomass crops for a resource-constrained world. Springer, New YorkGoogle Scholar
- Beckett M, Loreto F, Velikova V, Brunetti C, Di Ferdinando M, Tattini M, Calfapietra C, Farrant JM (2012) Photosynthetic limitations and volatile and non-volatile isoprenoids in the poikilochlorophyllous resurrection plant Xerophyta humilis during dehydration and rehydration. Plant, Cell Environ 35:2061–2074CrossRefGoogle Scholar
- Brilli F, Tsonev T, Mahmood T, Velikova V, Loreto F, Centritto M (2013) Ultradian variation of isoprene emission, photosynthesis, mesophyll conductance and optimum temperature sensitivity for isoprene emission in water-stressed Eucalyptus citriodora saplings. J Exp Bot 64:519–528CrossRefPubMedGoogle Scholar
- Christou A, Manganaris GA, Papadopoulos I, Fotopoulos V (2013) Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defense pathways. J Exp Bot 64:1953–1966CrossRefPubMedPubMedCentralGoogle Scholar
- Dahnke WC, Whitney DA (1988) Measurement of soil salinity. In: Recommended soil chemical test procedures for the North Central Region. North Central Regional Research Publication No. 221 (Revised). North Dakota Agricultural Experiment Station Bulletin 499, Fargo, pp 32–34Google Scholar
- Haworth M, Catola S, Marino G, Brunetti C, Michelozzi M, Riggi E, Avola G, Cosentino SL, Loreto F, Centritto M (2017b) Moderate drought stress induces increased foliar dimethylsulphoniopropionate (DMSP) concentration and isoprene emission in two contrasting ecotypes of Arundo donax. Front Plant Sci 8:1016. https://doi.org/10.3389/fpls201701016 CrossRefPubMedPubMedCentralGoogle Scholar
- Marino G, Brunetti C, Tattini M, Romano A, Biasioli F, Tognetti R, Loreto F, Ferrini F, Centritto M (2017) Dissecting the role of isoprene and stress-related hormones, ABA and ethylene, signaling in split-root Populus nigra exposed to water stress. Tree Physiol. https://doi.org/10.1093/treephys/tpx083 PubMedGoogle Scholar
- Olowu RA, Adewuyi GO, Onipede OJ, Lawal OA, Sunday OM (2015) Concentration of heavy metals in root, stem and leaves of Acalypha indica and Panicum maximum jacq from three major dumpsites in Ibadan Metropolis, South West Nigeria. Am J Chem 5:40–48Google Scholar
- Quiroga G, Erice G, Aroca R, Chaumont F, Ruiz-Lozano JM (2017) Enhanced drought stress tolerance by the arbuscular mycorrhizal symbiosis in a drought-sensitive maize cultivar is related to a broader and differential regulation of host plant aquaporins than in a drought-tolerant cultivar. Front Plant Sci 8:1056. https://doi.org/10.3389/fpls201701056 CrossRefPubMedPubMedCentralGoogle Scholar
- Romero-Munar A, Del-Saz NF, Ribas-Carbó M, Flexas J, Baraza E, Florez-Sarasa I, Fernie AR, Gulías J (2017) Arbuscular mycorrhizal symbiosis with Arundo donax decreases root respiration and increases both photosynthesis and plant biomass accumulation. Plant, Cell Environ 40:1115–1126CrossRefGoogle Scholar
- Tattini M, Loreto F, Fini A, Guidi L, Brunetti C, Velikova V, Gori A, Ferrini F (2015) Isoprenoids and phenylpropanoids are part of the antioxidant defense orchestrated daily by drought-stressed Platanus × acerifolia plants during Mediterranean summers. New Phytol 37:1950–1964Google Scholar
- Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986) Estimation of VA mycorrhizal infection levels Research for methods having a functional significance. Proceedings of the first European symposium, physiological and genetical aspects of mycorrhizae. Dijon Centre National de la Recherche Scientifique, Dijon; Institut National de la Recherche Agronomique, Dijon; Station d’Amelioration des Plantes, Paris, pp 217–221Google Scholar
- Unno H, Maeda Y, Yamamoto S, Okamoto M, Takenaga H (2002) Relationship between salt tolerance and Ca2+ retention among plant species. Jpn J Soil Sci Plant Nutr 73:715–718Google Scholar