Effect of salt stress on fatty acid and α-tocopherol metabolism in two desert shrub species
- 365 Downloads
Compared to Artemisia ordosiea Kraschen, a higher content of α-tocopherol in Artemisia sphaerocephala Kraschen under salt stress inhibits the conversion of linoleic acid (C18:2) into linolenic acid (C18:3), maintains cell membrane stability and contributes to higher salt resistance.
Artemisia sphaerocephala Kraschen and Artemisia ordosiea Kraschen are widely distributed in the arid and semiarid desert regions of the northwest of China. Under salt stress, it has been known that α-tocopherol (α-T) improves membrane permeability and maintains Na+/K+ homeostasis; however, the function of α-T in regulating membrane components of fatty acids is unknown. In this study, 100-day-old plants of A. ordosiea and A. sphaerocephala are subjected to various NaCl treatments for 7, 14, and 21 days. Compared to A. ordosiea, A. sphaerocephala has a higher Na+ concentration, higher chlorophyll content and dry weight in all NaCl treatments, but lower relative electric conductivity. The stable unsaturated levels of the lipids in A. sphaerocephala may be attributed to higher level of C18:2. Under 200 mM NaCl treatment, α-T and C18:2 contents in A. sphaerocephala increase significantly, while the Na+, C18:1, C18:3 and jasmonic acid (JA) contents decrease. Moreover, α-T is positively correlated with C18:2, but negatively correlated with C18:3.
KeywordsMembrane permeability Salt stress α-Tocopherol Unsaturated fatty acid
Polyunsaturated fatty acid
The authors would like to thank Changgui Wan (Department of Natural Resources Management, Texas Technology University, USA) and Prof. Zengyu Wang (The Samuel Roberts Noble Foundation, USA) for the great help in English improving and discussion on possible mechanism. We also thank Haiyan Wen (State Key Laboratory of Grassland Agro-ecosystems, Lanzhou University) for data analysis. This work was supported by the National Basic Research Program of China (2014CB138703), National Key R&D Program of China (2016YFC0500506), National Natural Science Foundation of China (31770763), the Program for Chang Jiang Scholars and Innovative Research Team in University (IRT-17R50), and the Fundamental Research Funds for the Central Universities (lzujbky-2017-54).
- Board E (2010) Flora of China, vol 2-25. Science Press, BeijingGoogle Scholar
- Chrost B, Falk J, Kernebeck B, Mölleken H, Krupinska K (1999) Tocopherol biosynthesis in senescing chloroplasts—a mechanism to protect envelope membranes against oxidative stress and a prerequisite for lipid remobilization? In: Argyroudi-Akoyunoglou JH, Senger H (eds) The chloroplast: from molecular biology to biotechnology. Kluwer Academic Press, Dordrecht, pp 171–176CrossRefGoogle Scholar
- Farouk S (2011) Ascorbic acid and α-tocopherol minimize salt-induced wheat leaf senescence. J Physiol Biochem 7:58–79Google Scholar
- Filek M, Walas S, Mrowiec H, Rudolphy-Skórska E, Sieprawska A, Biesaga-Kościelniak J (2012) Membrane permeability and micro- and macroelement accumulation in spring wheat cultivars during the short-term effect of salinity- and PEG-induced water stress. Acta Physiol Plant 34:985–995CrossRefGoogle Scholar
- Hanway J, Heidel H (1952) Soil analysis methods as used in Iowa state college soil testing laboratory. Iowa Agric 57:1–31Google Scholar
- Jin S, Daniell H (2014) Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species. Plant Biotechnol J 12:1274–1285CrossRefPubMedPubMedCentralGoogle Scholar
- Koohafkan P, Stewart BA (2008) Water and cereals in drylands. Food and Agriculture Organization of the United Nations, EarthscanGoogle Scholar
- Rekha C, Reema C, Alka S (2012) Salt tolerance of Sorghum bicolor cultivars during germination and seedlinggrowth. Res J Rec Sci 1(3):1–10Google Scholar
- Salama KHA (2009) Amelioration of NaCl-induced alterations on the plasma membrane of Allium cepa L. by ascorbic acid. Austr J Basic Appl Sci 3(2):990–994Google Scholar
- Sárvári É, Balczer T, Szigeti Z, Záray G, Fodor F (2008) Effect of Cd on the iron re-supply-induced formation of chlorophyll-protein complexes in cucumber. Acta Biol Szeged 52:183–186Google Scholar
- Vom D, Hölzl G, Plohmann C, Eisenhut M, Abraham M, Weber APM, Hanson AD, Dörmann P (2015) Remobilization of phytol from chlorophyll degradation is essential for tocopherol synthesis and growth of Arabidopsis. Plant Cell 27(10):2846–2859Google Scholar
- Yusuf MA, Kumar D, Rajwanshi R, Strasser RJ, Tsimilli-Michael M, Govindjee Sarin NB (2010) Overexpression of γ-tocopherol methyl transferase gene in transgenic Brassica juncea plants alleviates abiotic stress: physiological and chlorophyll a fluorescence measurements. BBA (Bioenergetics) 1797:1428–1438CrossRefGoogle Scholar
- Zamani S, Bybordi A, Khorshidi MB, Nezami T (2010) Effects of NaCl salinity levels on lipids and proteins of canola (Brassica napus L.) cultivars. Adv Environ Res 28:197–206Google Scholar
- Zhang JT, Liu H, Sun J, Li B, Qiang Z, Chen SL, Zhang HX (2012) Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth. Plos One 7(7):e30355 1–e30355 12Google Scholar