Planta

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Effect of salt stress on fatty acid and α-tocopherol metabolism in two desert shrub species

Original Article
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Abstract

Main conclusions

Compared toArtemisia ordosieaKraschen, a higher content of α-tocopherol inArtemisia sphaerocephalaKraschen under salt stress inhibits the conversion of linoleic acid (C18:2) into linolenic acid (C18:3), maintains cell membrane stability and contributes to higher salt resistance.

Artemisia sphaerocephala Kraschen and Artemisia ordosiea Kraschen are widely distributed in the arid and semiarid desert regions of the northwest of China. Under salt stress, it has been known that α-tocopherol (α-T) improves membrane permeability and maintains Na+/K+ homeostasis; however, the function of α-T in regulating membrane components of fatty acids is unknown. In this study, 100-day-old plants of A. ordosiea and A. sphaerocephala are subjected to various NaCl treatments for 7, 14, and 21 days. Compared to A. ordosiea, A. sphaerocephala has a higher Na+ concentration, higher chlorophyll content and dry weight in all NaCl treatments, but lower relative electric conductivity. The stable unsaturated levels of the lipids in A. sphaerocephala may be attributed to higher level of C18:2. Under 200 mM NaCl treatment, α-T and C18:2 contents in A. sphaerocephala increase significantly, while the Na+, C18:1, C18:3 and jasmonic acid (JA) contents decrease. Moreover, α-T is positively correlated with C18:2, but negatively correlated with C18:3.

Keywords

Membrane permeability Salt stress α-Tocopherol Unsaturated fatty acid 

Abbreviations

α-T

α-Tocopherol

C18:1

Oleic acid

C18:2

Linoleic acid

C18:3

Linolenic acid

FA

Fatty acid

JA

Jasmonic acid

PM

Plasma membrane

PUFA

Polyunsaturated fatty acid

Supplementary material

425_2017_2803_MOESM1_ESM.jpg (2.6 mb)
Supplementary material 1 Supplemental Fig. S1 % Change over control of C18:2 in A. sphaerocephala (a) and A. ordosiea (b) leaves treated with 50, 100, 150 and 200 mM NaCl for various periods. Values are mean ± SD (n = 5) and bars indicate SD. Different letters within a column indicate significant difference at P < 0.05 (JPEG 2711 kb)
425_2017_2803_MOESM2_ESM.jpg (2.7 mb)
Supplementary material 2 Supplemental Fig. S2 % Change over control of C18:3 in A. sphaerocephala (a) and A. ordosiea (b) leaves treated with 50, 100, 150 and 200 mM NaCl for various periods. Values are mean ± SD (n = 5) and bars indicate SD. Different letters within a column indicate significant difference at P < 0.05 (JPEG 2714 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, National Demonstration Center for Experimental Grassland Science Education, College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouChina

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