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Planta

, Volume 246, Issue 5, pp 857–878 | Cite as

Non-cellulosic polysaccharide distribution during G-layer formation in poplar tension wood fibers: abundance of rhamnogalacturonan I and arabinogalactan proteins but no evidence of xyloglucan

  • Fernanda Trilstz Perassolo Guedes
  • Françoise Laurans
  • Bernard Quemener
  • Carole Assor
  • Véronique Lainé-Prade
  • Nathalie Boizot
  • Jacqueline Vigouroux
  • Marie-Claude Lesage-Descauses
  • Jean-Charles Leplé
  • Annabelle Déjardin
  • Gilles PilateEmail author
Original Article

Abstract

Main conclusion

RG-I and AGP, but not XG, are associated to the building of the peculiar mechanical properties of tension wood.

Hardwood trees produce tension wood (TW) with specific mechanical properties to cope with environmental cues. Poplar TW fibers have an additional cell wall layer, the G-layer responsible for TW mechanical properties. We investigated, in two poplar hybrid species, the molecules potentially involved in the building of TW mechanical properties. First, we evaluated the distribution of the different classes of non-cellulosic polysaccharides during xylem fiber differentiation, using immunolocalization. In parallel, G-layers were isolated and their polysaccharide composition determined. These complementary approaches provided information on the occurrence of non-cellulosic polysaccharides during G-fiber differentiation. We found no evidence of the presence of xyloglucan (XG) in poplar G-layers, whereas arabinogalactan proteins (AGP) and rhamnogalacturonan type I pectins (RG-I) were abundant, with an apparent progressive loss of RG-I side chains during G-layer maturation. Similarly, the intensity of immunolabeling signals specific for glucomannans and glucuronoxylans varies during G-layer maturation. RG-I and AGP are best candidate matrix components to be responsible for TW mechanical properties.

Keywords

Cellulose aggregation G-fiber maturation Hemicellulose Hydrogel formation Mechanical strain Pectin 

Abbreviations

AGP

Arabinogalactan protein

HG

Homogalacturonan

HPAEC-PAD

High-performance anion exchange chromatography with pulsed amperometric detection

MALDI-TOF MS

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry

ML

Middle lamella

OW

Opposite wood

PCW

Primary cell wall

RG-I (II)

Rhamnogalacturonan type I (type II) pectin

TW

Tension wood

XG

Xyloglucan

XGO

Xyloglucan oligomers

Notes

Acknowledgements

This work was partly supported by the “Stress in Trees” Project ANR-12-BS09-0004. FTP Guedes was funded by CAPES (Ministry of Education, Brazil). We would like to thank Pierre-Ivan Raynal (Electron Microscopy Facility, François Rabelais University and University Hospital of Tours, France) for technical support for MET observations. Confocal microscopy was carried out in Xylobiotech Facility funded by the IA project “Xyloforest” (ANR-10-EQPX-16).

Supplementary material

425_2017_2737_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 16 kb)
425_2017_2737_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 19 kb)
425_2017_2737_MOESM3_ESM.docx (587 kb)
Supplementary material 3 (DOCX 586 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Fernanda Trilstz Perassolo Guedes
    • 1
    • 3
  • Françoise Laurans
    • 1
  • Bernard Quemener
    • 2
  • Carole Assor
    • 2
    • 4
  • Véronique Lainé-Prade
    • 1
  • Nathalie Boizot
    • 1
  • Jacqueline Vigouroux
    • 2
  • Marie-Claude Lesage-Descauses
    • 1
  • Jean-Charles Leplé
    • 1
  • Annabelle Déjardin
    • 1
  • Gilles Pilate
    • 1
    Email author
  1. 1.AGPF, INRAOrléansFrance
  2. 2.BIA, INRANantesFrance
  3. 3.Faber-CastellSão CarlosBrazil
  4. 4.IATE, INRAMontpellierFrance

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