Non-cellulosic polysaccharide distribution during G-layer formation in poplar tension wood fibers: abundance of rhamnogalacturonan I and arabinogalactan proteins but no evidence of xyloglucan
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RG-I and AGP, but not XG, are associated to the building of the peculiar mechanical properties of tension wood.
Hardwood trees produce tension wood (TW) with specific mechanical properties to cope with environmental cues. Poplar TW fibers have an additional cell wall layer, the G-layer responsible for TW mechanical properties. We investigated, in two poplar hybrid species, the molecules potentially involved in the building of TW mechanical properties. First, we evaluated the distribution of the different classes of non-cellulosic polysaccharides during xylem fiber differentiation, using immunolocalization. In parallel, G-layers were isolated and their polysaccharide composition determined. These complementary approaches provided information on the occurrence of non-cellulosic polysaccharides during G-fiber differentiation. We found no evidence of the presence of xyloglucan (XG) in poplar G-layers, whereas arabinogalactan proteins (AGP) and rhamnogalacturonan type I pectins (RG-I) were abundant, with an apparent progressive loss of RG-I side chains during G-layer maturation. Similarly, the intensity of immunolabeling signals specific for glucomannans and glucuronoxylans varies during G-layer maturation. RG-I and AGP are best candidate matrix components to be responsible for TW mechanical properties.
KeywordsCellulose aggregation G-fiber maturation Hemicellulose Hydrogel formation Mechanical strain Pectin
High-performance anion exchange chromatography with pulsed amperometric detection
- MALDI-TOF MS
Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry
Primary cell wall
- RG-I (II)
Rhamnogalacturonan type I (type II) pectin
This work was partly supported by the “Stress in Trees” Project ANR-12-BS09-0004. FTP Guedes was funded by CAPES (Ministry of Education, Brazil). We would like to thank Pierre-Ivan Raynal (Electron Microscopy Facility, François Rabelais University and University Hospital of Tours, France) for technical support for MET observations. Confocal microscopy was carried out in Xylobiotech Facility funded by the IA project “Xyloforest” (ANR-10-EQPX-16).
- Fujita M, Saiki H, Harada H (1974) Electron microscopy of microtubules and cellulose microfibrils in secondary wall formation of poplar tension wood fibers. Mokuzai Gakkaishi 20:147–156Google Scholar
- Gorshkova T, Mokshina NE, Chernova TE, Ibragimova NN, Salnikov VV, Mikshina PV, Tryfona T, Banasiak A, Immerzeel P, Dupree P, Mellerowicz E (2015) Aspen tension wood fibers contain β-(1 → 4)-galactans and acidic arabinogalactans retained by cellulose microfibrils in gelatinous walls. Plant Physiol 169:2048–2063PubMedPubMedCentralGoogle Scholar
- Knox JP, Peart J, Neill SJ (1995) Identification of novel cell surface epitopes using leaf epidermal strip assay system. Planta 196:266–270Google Scholar
- Koutaniemi S, Guillon F, Tranquet O, Bouchet B, Tuomainen P, Virkki L, Petersen HL, Willats WG, Saulnier L, Tenkanen M (2012) Substituent-specific antibody against glucuronoxylan reveals close association of glucuronic acid and acetyl substituents and distinct labeling patterns in tree species. Planta 236:739–751CrossRefPubMedGoogle Scholar
- Macquet A, Ralet MC, Loudet O, Kronenberger J, Mouille G, Marion-Poll A, North HM (2007) A naturally occurring mutation in an Arabidopsis accession affects a beta-d-galactosidase that increases the hydrophilic potential of rhamnogalacturonan I in seed mucilage. Plant Cell 19:3990–4006CrossRefPubMedPubMedCentralGoogle Scholar
- Nishikubo N, Awano T, Banasiak A, Bourquin V, Ibatullin F, Funada R, Brumer H, Teeri TT, Hayashi T, Sundberg B, Mellerowicz EJ (2007) Xyloglucan endo-transglycosylase (XET) functions in gelatinous layers of tension wood fibers in poplar—a glimpse into the mechanism of the balancing act of trees. Plant Cell Physiol 48:843–855CrossRefPubMedGoogle Scholar
- Puhlmann J, Bucheli E, Swain MJ, Dunning N, Albersheim P, Darvill AG, Hahn MG (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1-2)-linked fucosyl-containing epitope. Plant Physiol 104:699–710CrossRefPubMedPubMedCentralGoogle Scholar
- Richardson KC, Jarett L, Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Biotech Histochem 35:313–323Google Scholar
- Tan L, Eberhard S, Pattathil S, Warder C, Glushka J, Yuan CH, Hao ZY, Zhu X, Avci U, Miller JS, Baldwin D, Pham C, Orlando R, Darvill A, Hahn MG, Kieliszewski MJ, Mohnen D (2013) An Arabidopsis cell wall proteoglycan consists of pectin and arabinoxylan covalently linked to an arabinogalactan protein. Plant Cell 25:270–287CrossRefPubMedPubMedCentralGoogle Scholar
- Timell TE (1969) The chemical composition of tension wood. Sven Papperstidn 72:173–181Google Scholar