Planta

, Volume 246, Issue 4, pp 673–685 | Cite as

Allelopathic effects of exogenous phenylalanine: a comparison of four monocot species

  • Barbara R. Evans
  • Garima Bali
  • Art Ragauskas
  • Riddhi Shah
  • Hugh O’Neill
  • Cory Howard
  • Fayola Lavenhouse
  • Dawn Ramirez
  • Kelly Weston
  • Kelly Ramey
  • Valerie Cangemi
  • Brian Kinney
  • Claudia Partee
  • Teresa Ware
  • Brian Davison
Original Article
  • 349 Downloads

Abstract

Main conclusion

Exogenous phenylalanine stunted annual ryegrass but not switchgrass or winter grain rye, with deuterium incorporation up to 3% from phenyalanine-d8. Toxicity to duckweed varied with illumination intensity and glucose uptake.

Isotopic labeling of biomolecules through biosynthesis from deuterated precursors has successfully been employed for both structural studies and metabolic analysis. Phenylalanine is the precursor of many products synthesized by plants, including the monolignols used for synthesis of lignin. Possible allelochemical effects of phenylalanine have not been reported, although its deamination product cinnamic acid is known to have deleterious effects on root elongation and growth of several plant species. The effects of phenylalanine and its deuterated analog phenylalanine-d8 added to growth media were studied for annual ryegrass (Lolium multiflorum), winter grain rye (Secale cereale), and switchgrass (Panicum virgatum) cultivated under hydroponic conditions. Growth of annual ryegrass was inhibited by phenylalanine while switchgrass and rye were not significantly affected. Growth was less affected by deuterated phenylalanine-d8 than by its protiated counterpart, which may be a typical deuterium kinetic isotope effect resulting in slower enzymatic reaction rates. Deuterium incorporation levels of 2–3% were achieved in biomass of switchgrass and annual ryegrass. Both protiated and deuterated phenylalanine were moderately toxic (IC25 values 0.6 and 0.8 mM, respectively) to duckweed (Lemna minor) grown using a 12 h diurnal cycle under photoautotrophic conditions. A significant increase in toxicity, greater for the deuterated form, was noted when duckweed was grown under higher intensity, full spectrum illumination with a metal halide lamp compared to fluorescent plant growth lamps emitting in the blue and red spectral regions. Supplementation with glucose increased toxicity of phenylalanine consistent with synergy between hexose and amino acid uptake that has been reported for duckweed.

Keywords

Phenylalanine Allelopathy Deuterium Switchgrass Ryegrass Lemna 

Notes

Acknowledgements

This research was supported by the U. S. Department of Energy, Office of Science, through the Genomic Science Program, Office of Biological and Environmental Research, under Contract FWP ERKP752. The research at Oak Ridge National Laboratory’s Center for Structural Molecular Biology (CSMB) was supported by the U. S. Department of Energy, Office of Science, through the Office of Biological and Environmental Research under Contract FWP ERKP291, using facilities supported by the Office of Basic Energy Sciences, U. S. Department of Energy. R. Shah was partly supported by the graduate fellowship program of the Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville. C. Howard, F. Lavenhouse, and D. Ramirez, with K. Ramey as teacher-mentor, were supported by the Siemens Foundation through the Siemens Teachers As Research Scientists (STARS) summer 2014 program administered by Oak Ridge Institute of Science and Education, Oak Ridge Associated Universities. V. Cangemi, B. Kinney, C. Partee, and T. Ware were participants in the Appalachian Regional Commission/Oak Ridge National Laboratory Summer Math Science Technology Institute 2015 summer program. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U. S. Department of Energy under Contract DE-AC05-00OR22725.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

425_2017_2720_MOESM1_ESM.pdf (1.4 mb)
Supplementary material 1 (PDF 1435 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Barbara R. Evans
    • 1
  • Garima Bali
    • 2
  • Art Ragauskas
    • 2
    • 3
    • 4
    • 5
  • Riddhi Shah
    • 6
    • 7
  • Hugh O’Neill
    • 6
  • Cory Howard
    • 8
  • Fayola Lavenhouse
    • 8
  • Dawn Ramirez
    • 8
  • Kelly Weston
    • 8
  • Kelly Ramey
    • 8
  • Valerie Cangemi
    • 9
  • Brian Kinney
    • 9
  • Claudia Partee
    • 9
  • Teresa Ware
    • 9
  • Brian Davison
    • 5
  1. 1.Chemical Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  2. 2.Institute of Paper Science and TechnologySchool of Chemistry and Biochemistry, Georgia Institute of TechnologyAtlantaUSA
  3. 3.Department of Chemical and Biomolecular EngineeringUniversity of TennesseeKnoxvilleUSA
  4. 4.Department of Forestry, Wildlife, and Fisheries, Center for Renewable CarbonUniversity of Tennessee Institute of AgricultureKnoxvilleUSA
  5. 5.Biosciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  6. 6.Biology and Soft Matter DivisionOak Ridge National LaboratoryOak RidgeUSA
  7. 7.Bredesen Center for Interdisciplinary Research and Graduate EducationUniversity of TennesseeKnoxvilleUSA
  8. 8.Siemens Teachers As Research Scientists (STARS) High School Science Teacher Summer Research Experience ProgramOak Ridge Institute of Science and Education, Oak Ridge Associated UniversitiesOak RidgeUSA
  9. 9.Participants in Appalachian Regional Commission (ARC) High School Science Teacher Summer Research Experience ProgramOak Ridge National LaboratoryOak RidgeUSA

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