Planta

, Volume 246, Issue 1, pp 105–122 | Cite as

Rice fatty acyl-CoA synthetase OsACOS12 is required for tapetum programmed cell death and male fertility

  • Xijia Yang
  • Wanqi Liang
  • Minjiao Chen
  • Dabing Zhang
  • Xiangxiang Zhao
  • Jianxin Shi
Original Article

Abstract

Main Conclusion

Loss of function mutation of rice OsACOS12 impairs lipid metabolism-mediated anther cuticle and pollen wall formation, and interferes with tapetum programmed cell death, leading to male sterility.

Acyl-CoA Synthetase (ACOS) is one of the enzymes activating fatty acids for various metabolic functions in plants. Here, we show that OsACOS12, an orthologue of Arabidopsis ACOS5 in rice, is crucial for rice fertility. Similar to acos5, osaocs12 mutant had no mature pollen. But unlike acos5, osaocs12 produced defective anthers lacking cutin and Ubisch bodies on the epidermal and inner surfaces, respectively, and delayed programmed cell death (PCD)-induced tapetum degradation. Those phenotypic changes were evident at stage 10, during which OsACOS12 had its maximum expression in tapetal cells and microspores. Chemical analysis revealed that the levels of anther cuticular lipid components (wax and cutin monomers) were significantly reduced in osaocs12, while the expression levels of three known lipid biosynthetic genes were unchanged. Recombinant OsACOS12 enzyme was shown to catalyze the conversion of C18:1 fatty acid to C18:1 CoA in vitro. Phylogenetic analysis indicated that OsACOS12 is an ancient and conserved enzyme associated with the plant’s colonization to earth. Collectively, our study suggests that OsACOS12 is an ancient enzyme participating in a conserved metabolic pathway for diversified biochemical functions to secure male reproduction in plants.

Keywords

Acyl-activating enzyme Anther cuticle Male sterility Oryza sativa Pollen exine 

Abbreviations

FID

Flame ionization detector

GC–MS

Gas chromatography–mass spectrometry

GUS

β-Glucuronidase

PCD

Programmed cell death

HPLC–MS/MS

High-performance liquid chromatography–mass spectrometer/mass spectrometry

SEM

Scanning electron microscopy

TEM

Transmission electron microscopy

TUNEL

Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling

Supplementary material

425_2017_2691_MOESM1_ESM.docx (1.4 mb)
Supplementary material 1 (DOCX 1472 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Xijia Yang
    • 1
  • Wanqi Liang
    • 1
  • Minjiao Chen
    • 1
  • Dabing Zhang
    • 1
    • 2
    • 3
  • Xiangxiang Zhao
    • 3
  • Jianxin Shi
    • 1
    • 3
  1. 1.Joint International Research Laboratory of Metabolic and Developmental SciencesShanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityShanghaiChina
  2. 2.Plant Genomics Center, School of Agriculture, Food and WineUniversity of AdelaideUrrbraeAustralia
  3. 3.Key Laboratory of Crop Marker-Assisted Breeding of Huaian MunicipalityJiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental ProtectionHuaianChina

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