Planta

, Volume 245, Issue 6, pp 1215–1229 | Cite as

Lettuce flavonoids screening and phenotyping by chlorophyll fluorescence excitation ratio

  • Marek Zivcak
  • Klaudia Brückova
  • Oksana Sytar
  • Marian Brestic
  • Katarina Olsovska
  • Suleyman I. Allakhverdiev
Original Article

Abstract

Main conclusion

Environmentally induced variation and the genotypic differences in flavonoid and phenolic content in lettuce can be reliably detected using the appropriate parameters derived from the records of rapid non-invasive fluorescence technique.

The chlorophyll fluorescence excitation ratio method was designed as a rapid and non-invasive tool to estimate the content of UV-absorbing phenolic compounds in plants. Using this technique, we have assessed the dynamics of accumulation of flavonoids related to developmental changes and environmental effects. Moreover, we have tested appropriateness of the method to identify the genotypic differences and fluctuations in total phenolics and flavonoid content in lettuce. Six green and two red genotypes of lettuce (Lactuca sativa L.) grown in pots were exposed to two different environments for 50 days: direct sunlight (UV-exposed) and greenhouse conditions (low UV). The indices based on the measurements of chlorophyll fluorescence after red, green and UV excitation indicated increase of the content of UV-absorbing compounds and anthocyanins in the epidermis of lettuce leaves. In similar, the biochemical analyses performed at the end of the experiment confirmed significantly higher total phenolic and flavonoid content in lettuce plants exposed to direct sun compared to greenhouse conditions and in red compared to green genotypes. As the correlation between the standard fluorescence indices and the biochemical records was negatively influenced by the presence of red genotypes, we proposed the use of a new parameter named Modified Flavonoid Index (MFI) taking into an account both absorbance changes due to flavonol and anthocyanin content, for which the correlation with flavonoid and phenolic content was relatively good. Thus, our results confirmed that the fluorescence excitation ratio method is useful for identifying the major differences in phenolic and flavonoid content in lettuce plants and it can be used for high-throughput pre-screening and phenotyping of leafy vegetables in research and breeding applications towards improvement of vegetable health effects.

Keywords

Phenolic Flavonoids Chlorophyll fluorescence Lettuce UV radiation Phenotyping 

Abbreviations

ANTH

Fluorescence-based index for estimation of anthocyanin content in plant tissues

Anth

Anthocyanins

DW

Dry weight

FER

Fluorescence excitation ratio

FLAV

Fluorescence-based index for estimation of flavonol content in plant tissues

Flav

Flavonoids

FLAVC

Corrected fluorescence-based index for estimation of flavonol content

FRF

Far-red fluorescence, fluorescence with wavelength ~730 nm

FRFG

Far-red fluorescence emitted after excitation by green light

FRFR

Far-red fluorescence emitted after excitation by red light

FRFUV

Far-red fluorescence emitted after excitation by UV

MFI

Modified flavonoid index

Notes

Acknowledgements

This work was supported by the research projects VEGA-1-0923-16, APVV-15-0721,  by the EC project no. 26220220180: “Construction of the “AgroBioTech” Research Centre”, by Grants from the Russian Foundation for Basic Research, and by the Molecular and Cell Biology Programs of the Russian Academy of Sciences.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of Plant PhysiologySlovak Agricultural UniversityNitraSlovak Republic
  2. 2.Agrobiotech Research CenterSlovak Agricultural UniversityNitraSlovak Republic
  3. 3.Educational and Scientific Centre, Institute of Biology and MedicineTaras Shevchenko National University of KyivKyivUkraine
  4. 4.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  5. 5.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia
  6. 6.Department of Plant Physiology, Faculty of BiologyM.V. Lomonosov Moscow State UniversityMoscowRussia
  7. 7.Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of SciencesBakuAzerbaijan

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