Molecular cytogenetic characterization of a new wheat-rye 1BL•1RS translocation line expressing superior stripe rust resistance and enhanced grain yield
- 728 Downloads
A new wheat-rye 1BL•1RS translocation line, with the characteristics of superior stripe rust resistance and high thousand-kernel weight and grain number per spike, was developed and identified from progenies of wheat-rye- Psathyrostachys huashanica trigeneric hybrids.
The wheat-rye 1BL•1RS translocation line from Petkus rye has contributed substantially to the world wheat production. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. In this study, a new wheat-rye line K13-868, derived from the progenies of wheat-rye-Psathyrostachys huashanica trigeneric hybrids, was identified and analyzed using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), acid polyacrylamide gel electrophoresis (A-PAGE), and molecular markers. Cytological studies indicated that the mean chromosome configuration of K13-868 at meiosis was 2n = 42 = 0.14 I + 18.78 II (ring) + 2.15 II (rod). Sequential FISH and GISH results demonstrated that K13-868 was a compensating wheat-rye 1BL•1RS Robertsonian translocation line. Acid PAGE analysis revealed that clear specific bands of rye 1RS were expressed in K13-868. Simple sequence repeat (SSR) and rye 1RS-specific markers ω-sec-p1/ω-sec-p2 and O-SEC5′-A/O-SEC3′-R suggested that the 1BS arm of wheat had been substituted by the 1RS arm of rye. At the seedling and adult growth stage, compared with its recurrent wheat parent SM51 and six other wheat cultivars containing the 1RS arm in southwestern China, K13-868 showed high levels of resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, which are virulent to Yr10 and Yr24/Yr26. In addition, K13-868 expresses higher thousand-kernel weight and more grain number per spike than these controls in two growing seasons, suggesting that this line may carry yield-related genes of rye. This translocation line, with significant characteristics of resistance to stripe rust and high thousand-kernel weight and grain number per spike, could be utilized as a valuable germplasm for wheat improvement.
Keywords1BL•1RS translocation line Grain number per spike Sequential FISH and GISH Stripe rust resistance Thousand-kernel weight
Genome in situ hybridization
Fluorescence in situ hybridization
Simple sequence repeat
Puccinia striiformis f. sp. tritici
This work was funded by the National Natural Science Foundation of China (No. 31501311), the National High Technology Research and Development Program of China (863 program, No. 2011AA100103), and the Science and Technology Bureau and Education Bureau of Sichuan Province. We thank Dr. Tang ZX (Sichuan Agricultural University, China) for technical guidance in FISH analysis. We also thank Dr. Steven S. Xu, Red River Valley Agricultural Research Center, USDA-ARS, USA, for help with manuscript improvement.
Compliance with ethical standards
Conflict of interest
We declare that we have no conflict of interest.
- Bartoš P (1993) Chromosome 1R of rye in wheat breeding. Plant Breed 63:1203–1211Google Scholar
- Cook RJ (1987) The classification of wheat cultivars using a standard reference electrophoresis method. J Nat Agric Bot 17:273–281Google Scholar
- Dong YS, Zhou RH, Xu SJ, Cauderon Y, Wang RC (1992) Desirable characteristics in perennial Triticeae collected in China for wheat improvement. Hereditas 116:176–178Google Scholar
- Evans LT (1996) Crop evolution, adaptation and yield. Cambridge University PressGoogle Scholar
- Han DJ, Wang QL, Zhang L, Kang ZS (2010) Evaluation of resistance of current wheat cultivars to stripe rust in northwest China, north China and the middle and lower reaches of Changjiang River epidemic area. Sci Agric Sin 43:2889–2896Google Scholar
- Klindworth DL, Niu Z, Chao S, Friesen TL, Jin Y, Faris JD, Cai X, Xu SS (2012) Introgression and characterization of a goatgrass gene for a high level of resistance to Ug99 stem rust in tetraploid wheat. Genes Genome Genet 2:665–673Google Scholar
- Liu XK (1988) Study on the yellow rust resistance to common wheat (T. aestivum). Plant Prot 15:33–39Google Scholar
- Lukaszewski AJ (2000) Manipulation of the 1RS∙1BL translocation in wheat by induced homoeologous recombination. Crop Sci:216–225Google Scholar
- Mujeeb-Kazi A, Kazi AG, Dundas I, Rasheed A, Ogbonnaya F, Kishi M, Bonnett D, Wang RRC, Xu S, Chen P, Mahmood T, Bux H, Farrakh S (2013) Genetic diversity for wheat improvement as a conduit for food security. In: Sparks D (ed) Advances in agronomy, vol 122. Academic Press, Burlington, pp 179–257Google Scholar
- Qi LL, Pumphrey MO, Friebe B, Zhang P, Chen Q, Bowden RL, Rouse MN, Jin Y, Gill BS (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167CrossRefPubMedGoogle Scholar
- Xiao Y, Liu X, He S, Xia X, He Z, Ji W (2011) Analyses of genetic effect of 1BL. 1RS translocation on kernel traits in common wheat. J Northwest Agricul For Univ 5:24Google Scholar
- Xie Q, Kang HY, Tao S, SparkesDL Fan XM, Cui ZG, Xu LL, HuangJ Fan X, Sha LN, Zhang HQ, Zhou YH (2012) Wheat lines derived from trigeneric hybrids of wheat-rye-Psathyrostachys huashanica, the potential resources for grain weight improvement. Aust J Crop Sci 6:1550–1557Google Scholar
- Zeller FJ (1973) 1B/1R wheat chromosome substitutions and translocations. In: Sears ER, Sears LMS (eds) Proceedings of 4th international wheat genetic symptoms. Columbia USA, pp 209–221Google Scholar
- Zhan HX, Li GR, Zhang XJ, Li X, Guo HJ, Gong WP, Jia JQ, Qiao LY, Ren YK, Yang ZJ, Chang ZJ (2014) Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat-Thinopyrum ponticum introgression line. PLoS ONE 9(11):e113455CrossRefPubMedPubMedCentralGoogle Scholar
- Zhou Y, He ZH, Zhang GS, Xia LQ, Chen XM, Gao YC, Jin ZB, Yu GJ (2004) Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agron Sin 30:531–535Google Scholar