A SCARECROW-based regulatory circuit controls Arabidopsis thaliana meristem size from the root endodermis
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SCARECROW controls Arabidopsis root meristem size from the root endodermis tissue by regulating the DELLA protein RGA that in turn mediates the regulation of ARR1 levels at the transition zone.
Coherent organ growth requires a fine balance between cell division and cell differentiation. Intriguingly, plants continuously develop organs post-embryonically thanks to the activity of meristems that allow growth and environmental plasticity. In Arabidopsis thaliana, continued root growth is assured when division of the distal stem cell and their daughters is balanced with cell differentiation at the meristematic transition zone (TZ). We have previously shown that at the TZ, the cytokinin-dependent transcription factor ARR1 controls the rate of differentiation commitment of meristematic cells and that its activities are coordinated with those of the distal stem cells by the gene SCARECROW (SCR). In the stem cell organizer (the quiescent center, QC), SCR directly suppresses ARR1 both sustaining stem cell activities and titrating non-autonomously the ARR1 transcript levels at the TZ via auxin. Here, we show that SCR also exerts a fine control on ARR1 levels at the TZ from the endodermis by sustaining gibberellin signals. From the endodermis, SCR controls the RGA REPRESSOR OF ga1-3 (RGA) DELLA protein stability throughout the root meristem, thus controlling ARR1 transcriptional activation at the TZ. This guarantees robustness and fineness to the control of ARR1 levels necessary to balance cell division to cell differentiation in sustaining coherent root growth. Therefore, this work advances the state of the art in the field of root meristem development by integrating the activity of three hormones, auxin, gibberellin, and cytokinin, under the control of different tissue-specific activities of a single root key regulator, SCR.
KeywordsARABIDOPSIS RESPONSE REGULATOR 1 (ARR1) Differentiation Gibberellin Hormones Root SCARECROW (SCR)
ARABIDOPSIS RESPONSE REGULATOR 1
REPRESSOR OF ga1-3
Stem cell niche
The ability of plants to generate organs continuously depends on the activity of meristems, localized regions where plant stem cells reside (reviewed in Heidstra and Sabatini 2014). The root meristem is responsible for generating the root system, which anchors the plant to the soil while providing all the necessary nutrients. Longitudinally, the root meristem can be divided into three functionally distinct zones: a distal stem cell niche (SCN) generating transit-amplifying cells, which divide in the proximal meristem (PM) and differentiate in the elongation/differentiation zone (EDZ) (reviewed in Heidstra and Sabatini 2014). The boundary where cells leave the meristem and enter the EDZ is called transition zone (TZ). To maintain root meristem activity and thus root growth, the activities of these zones must be well coordinated, and different plant hormones have been shown to play a crucial role in ensuring this coordination. For example, the balance between cell division in the PM and cell differentiation in the EDZ depends on the antagonistic interaction between cytokinin, promoting cell differentiation, and auxin, prompting cell division (Dello Ioio et al. 2007, 2008). Cytokinin promotes cell differentiation via the cytokinin-dependent response transcription factor ARABIDOPSIS RESPONSE REGULATOR 1 (ARR1) (Dello Ioio et al. 2007; Taniguchi et al. 2007), which in turn activates the auxin signaling inhibitor gene SHY2 (Tian et al. 2002; Dello Ioio et al. 2008). Another plant hormone, gibberellin (GA) (Olszewski et al. 2002; Hedden and Sponsel 2015), has been reported to control cell division in the PM acting from the root endodermis (Achard et al. 2009; Ubeda-Tomás et al. 2009). In particular, it was shown that GA sustains cell division by suppressing the ARR1 transcript level at the TZ via the DELLA protein REPRESSOR OF GA1-3 (RGA) (Silverstone et al. 1998; Moubayidin et al. 2010).
A single gene, SCARECROW (SCR) (Di Laurenzio et al. 1996), is responsible for the spatial coordination between stem cell activity in the SCN and differentiation of their daughters at the TZ (Moubayidin et al. 2013). The SCR protein, a member of the GRAS family of transcription factors, is specifically expressed in the stem cell niche organizing cells, the quiescent center (QC), and in the endodermis (Di Laurenzio et al. 1996; Pysh et al. 1999; Wysocka-Diller at al. 2000; Lee et al. 2008). Besides its role in sustaining the stem cells’ self-renewal activity (Sabatini et al. 2003; Moubayidin et al. 2013) SCR is known to be involved in many other root developmental events such as regulation of formative stem cell division (Heidstra et al. 2004; Cui et al. 2007; Sozzani et al. 2010; Cruz-Ramírez et al. 2012) and protoxylem formation (Carlsbecker et al. 2010).
SCR sustains stem cell activities by directly suppressing ARR1 expression in the QC cells, thus titrating auxin production (Moubayidin et al. 2013). Auxin produced in the QC not only controls stem cell division activity but at the same time acts, via polar transport, as a long-distance signal to fine-tune the level of ARR1 transcription in the TZ (Moubayidin et al. 2013). In this way, ARR1 is positioned at the TZ where cell differentiation is initiated (Moubayidin et al. 2013).
Here, we report that in addition to controlling ARR1 position and activity via auxin (Moubayidin et al. 2013) SCR controls ARR1 levels also through GA from the endodermis, thus contributing to the fine-tuning of the ARR1-mediated cell differentiation commitment which is necessary to control root meristem size.
Materials and methods
Plant materials, growth conditions, and treatments
The Arabidopsis thaliana ecotypes Columbia (Col-0), Wassilewskija (Ws) and Landsberg erecta (Ler) were used. arr1-3 is in Col-0 background (Dello Ioio et al. 2007), scr-1 and sne-1 are in Ws background (Cui and Benfey 2009; Moubayidin et al. 2013), rga-24 is in Ler background (Moubayidin et al. 2010), pRGA:rga-Delta17 and pRGA:GFP:rga-Delta17 are in Ler background (Dill et al. 2001), ARR1:GUS, scr-1 ARR1:GUS and RGA:GFP were previously described (Dill et al. 2001; Mason et al. 2004; Moubayidin et al. 2010, 2013). Seeds were sterilized and grown as described previously (Perilli and Sabatini 2010). Gibberellin (GA3-Sigma) treatments were performed using 10 μM GA dissolved in ethanol, for 24 h on 5-day-old seedlings as previously described (Moubayidin et al. 2010). Experiments with scr-3 N9094 UAS::SCR:GR and relative control plants were performed by germinating seeds on plates containing 2 μM Dexamethasone (Dex-Sigma) for 5 days and then transferring the seedlings on plates containing 10 μM GA and 2 μM Dex for 24 h. pRGA:rga-Delta17 and pRGA:GFP:rga-Delta17 were obtained from Prof Tai-Ping Sun, Department of Biology, Duke University, Durham, NC, USA. sne-1 mutant (FLAG_461E03) was obtained from the Versailles Arabidopsis Stock Center, INRA, France.
Root length and meristem size analysis
For root length measurements, plates were photographed and the resulting images were analyzed using the ImageJ software available online (http://rsbweb.nih.gov/ij/) as described in Perilli and Sabatini (2010). Root meristem size is expressed as the number of cortex cells in a file extending from the quiescent center to the first elongated cortex cell, as described previously (Dello Ioio et al. 2007; Perilli and Sabatini 2010).
At the optical microscope, the distinction between dividing and differentiating root meristematic cells belonging to the cortex file is performed by the operator taking into account multiple parameters, i.e., the smaller size, the presence of a large central vacuole, and the denser cytoplasm of dividing meristematic cells versus differentiating ones. Moreover, both the position of the TZ in the outward tissue, the epidermis, and the extension of the lateral root cap are also evaluated as helpful references to univocally identify the cortex TZ and, hence, to measure meristem size. For each experiment, a minimum of 90 plants was analyzed using an Axio Imager.A2 (Zeiss) light microscopy and average and standard deviation were calculated. To measure root development over time, root length and meristem size were analyzed at different days post-germination (dpg): 3 dpg, 5 dpg, 7 dpg, 9 dpg, 12 dpg, and 15 dpg.
GUS histochemical assay
To visualize ARR1:GUS line in wild-type and mutant backgrounds, GUS histochemical assay was performed using the β-glucuronidase substrate X-gluc (5-bromo-4-chloro-3-indolyl glucuronide, Duchefa) as previously described (Perilli and Sabatini 2010; Moubayidin et al. 2013). Five-day-old seedlings were incubated for 16 h at 37 °C in the dark and imaged using the Axio Imager.A2 (Zeiss) microscopy.
Total RNA was extracted from 5 days-old roots using the TRIsure reagent (Bioline), and the first strand cDNA was synthesized using the Superscript® III First Strand Synthesis System (Invitrogen). PCR amplification was carried out in the presence of the double-strand DNA-specific dye SYBR Green (Sigma). Amplification was monitored in real time with the 7300 Real-Time PCR System (Applied Biosystems). Experiments were performed in triplicates from RNA of root tissue. Amplification of ACTIN 2 served as control. Data are expressed in Log2 (2−ΔΔCt) ratio. qRT-PCR was performed three times on three independent RNA batches, and results were comparable in all experiments. Student t test was performed to know the actual significance of these data, the site used is: http://graphpad.com/quickcalcs/ttest2.cfm. Quantitative RT-PCR analysis was conducted using the following gene-specific primers:
ARR1 FW: 5′-TTGAAGAAACCGCGTGTCGTCT-3′ and ARR1 RV: 5′-CCTTCTCAACGCCGAGCTGATTAA-3′ for ARR1 (Moubayidin et al. 2013)
SNE FW: 5′-GTTCACCATGTCGTCGGAGA-3′ and
SNE RV: 5′-GAGCTCTGTTTCCGACAAGTG-3′ for SNEEZY (Sozzani et al. 2010)
RGA FW: 5′-CATGTTCCTCCACCGTCTTC-3′ and
RGA RV: 5′-AAAAAGGCAAAACCCTAGATC-3′ for RGA
ACT FW: 5′-CCTTCTCAACGCCGAGCTGATTAA-3′ and
ACT RV: 5′-GTGGATTCCAGCAGCTTCCAT-3′ for ACTIN2
Molecular cloning and genotyping
The SCR coding sequence lacking the stop codon was combined with the glucocorticoid receptor (GR) and cloned in the upstream activating sequence (UAS) cassette in between the 6xUAS and 35STerminator. Using the flanking NotI sites the UAS::SCR cassette was subsequently cloned into the pGreenII (Hellens et al. 2000)-based pGII124 binary vector (methotrexate resistance in planta) and transformed into scr-3 N9094 plants by floral dip.
The primers for genotyping plants for rga-24 deletion line were as follows:
RGA n212: 5′-GGTGATTTTCACGGTGGTTG-3′
rga-24 n205: 5′-TCGCTTAGTAGTTAGTACTC-3′
rga-24 n253: 5′-CATAGACCATAGTATTCGTGA-3′
RGA n212 and rga-24 n205 pair was used for amplifying the wild-type DNA copy of RGA, while rga-24 n205 and rga-24 n253 were used to amplify DNA from the mutant as described in de Lucas et al. (2008).
Confocal image processing
Confocal images of median longitudinal sections of 5-day-old roots were taken using a Zeiss LSM 780 microscope. A 10 mg/ml propidium iodide (Sigma) solution was used to visualize the cell wall.
SCR mediates GA-dependent control of root meristem size from the endodermis
GA is predicted to regulate root meristem size from the endodermis (Achard et al. 2009; Ubeda-Tomás et al. 2009) and in this tissue, SCR is known to directly activate the SNEEZY (SNE) gene (Cui et al. 2007), one of the F-box proteins necessary for gibberellin-mediated degradation of the RGA protein (Ariizumi and Steber 2011; Ariizumi et al. 2011). We thus wondered whether SCR controls root meristem size from the endodermis sustaining GA activity. To this aim we first exposed wild-type and scr-1 mutant seedlings to exogenous GA application, monitoring root meristem size after 24 h.
Root meristem size was measured as the number of meristematic cortex cells using manual labeling at the optical microscope (see “Materials and methods”) rather than the program Cell-o-Tape (French et al. 2012). In fact, while automating and speeding up operations, this program does not take into account changes neither in the density of the cytoplasm nor in the vacuole size of differentiating cells, important characteristics that allow, in our experience, a more precise positioning of the transition zone (Perilli and Sabatini 2010).
Upon GA treatments wild-type root meristem were enlarged, while no increase in meristem size was observed in the scr-1 mutant (Fig. 1c, e–h) suggesting that SCR activity is necessary to mediate GA-dependent control on root meristem size. To understand whether SCR functions in the endodermis to control root meristem size via GA we expressed the SCR gene in this tissue of the scr-3 mutant roots using a GAL4/UAS trans-activation system. In wild-type roots the N9094 (GAL4,UAS∷GFP) enhancer trap line from the J. Haseloff (http://www.plantsci.cam.ac.uk/Haseloff) collection, GFP, is expressed specifically in the cortex and endodermis tissues (Suppl. Fig. S1a) while in the scr mutant it is expressed in the monolayer typical of this mutant (Suppl. Fig. S1b) (Sabatini et al. 2003). The N9094 driver was introduced in scr-3 mutant plants carrying a UAS::SCR::GR construct where the SCR protein activities are inducible by Dexamethasone (Dex). In the resulting scr-3 N9094 UAS::SCR::GR(N9094≫SCR:GR) plants, GFP expression monitored GAL4 activity and hence ectopic SCR::GR expression in the mutant background. scr-3 N9094≫SCR:GR plants were germinated on Dex and were transferred on GA for 24 h. Analysis of the root meristem size of these plants revealed that the endodermis-specific induction of SCR was sufficient to enlarge the scr-3 meristem (Fig. 1b). Moreover, GA sensitivity was restored, as the meristem enlarged compared to GA-treated scr-3 roots (Fig. 1b). These data substantiate the notion that SCR mediates GA-dependent control of root meristem size functioning from the endodermis.
SCR mediates GA-dependent ARR1 transcriptional suppression
We have previously shown that GA controls root meristem size by lowering the expression of ARR1 at the TZ (Moubayidin et al. 2010) (Fig. 1c–f). We thus wondered whether SCR promotes the GA-mediated downregulation of ARR1 expression at the TZ, in addition to the regulation it has been shown to exert from the QC via auxin (Moubayidin et al. 2013).
Indeed, while GA negatively controls ARR1 transcription in the wild type (Fig. 1d–f) (Moubayidin et al. 2010), no downregulation of ARR1 expression was observed in the scr-1 mutant upon GA treatment as visualized both by quantitative real-time PCR (qRT-PCR) (Fig. 1d) and the analysis of scr-1 plants harboring a pARR1:ARR1:GUS (Fig. 1g, h). These data suggest that SCR is necessary to mediate the GA-dependent transcriptional downregulation of ARR1 in the control of root meristem size.
SCR mediates ARR1 transcriptional suppression via the RGA protein
Since GA acts via RGA to suppress ARR1 (Moubayidin et al. 2010) and SCR has been shown to directly control transcription of the specific RGA F-box protein SNE (Cui et al. 2007; Ariizumi and Steber 2011; Ariizumi et al. 2011), we hypothesized that the inability of GA to down-regulate ARR1 in the scr-1 mutant background could be due to increased levels of the RGA protein in the mutant.
Post-translational regulation of RGA controls root meristem size
ARR1 controls root meristem size in a dose-dependent manner
The data presented above suggest that the levels of ARR1 must be very finely regulated to determine root meristem size. We, therefore, set to establish whether a dose-dependent relation exists between the level of ARR1 and root meristem size by measuring the meristems of plant heterozygous for the scr-1 and arr1 mutations obtained by backcrossing the scr and arr1 mutants with the wild type. Five-day-old scr-1 heterozygous plants displayed root meristems shorter than Wt (Fig. 5f) whose roots kept growing over time. On the other hand, arr1 heterozygous plants displayed root meristems whose size was intermediate between wild-type plants and arr1 homozygous mutants (Fig. 5f), providing evidence for a dose-dependent control of meristem size by ARR1.
The number of transit-amplifying cells, which undergo a finite number of cell divisions in the proximal meristem (the division zone), can influence the rate of the overall root growth. Therefore, a tight control of the cell number within the meristem tunes the driving force of the root growth.
Author contribution statement
L.M. conceived the research, planned and performed experiments, analyzed the data and wrote the manuscript; E.S. planned and performed experiments; L.G. performed experiments; I.T. constructed and provided scr-3 N9094 UAS::SCR::GR (N9094≫SCR:GR) seeds; R.H. supervised experiments and provided critical review of the manuscript; P.C. discussed experiments and provided critical review of the manuscript; S.S. conceived the research, analyzed the data and wrote the manuscript.
We thank Prof Tai-Ping Sun for providing pRGA:rga-Delta17 and pRGA:GFP:rga-Delta17 mutants. We thank Prof Lars Østergaard, Dr Raffaele Dello Ioio and Dr Marta Del Bianco for reading the manuscript. This work was supported by The European Research Council (Grant number 260368 to S.S., L.M., E.S., L.G.), and by MIUR, Progetti di Ricerca di Interesse Nazionale (Grant number 2010HEBBB8 to P.C.).
Compliance with ethical standards
Conflict of interest
The authors declare no competing financial interests.
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