, Volume 243, Issue 3, pp 675–685 | Cite as

Towards the development of an oral vaccine against porcine cysticercosis: expression of the protective HP6/TSOL18 antigen in transgenic carrots cells

  • Elizabeth Monreal-Escalante
  • Dania O. Govea-Alonso
  • Marisela Hernández
  • Jacquelynne Cervantes
  • Jorge A. Salazar-González
  • Andrea Romero-Maldonado
  • Gabriela Rosas
  • Teresa Garate
  • Gladis Fragoso
  • Edda Sciutto
  • Sergio Rosales-Mendoza
Original Article


Main conclusion

The Taenia solium HP6/TSOL18 antigen was produced in carrot cells, yielding an immunogenic protein that induced significant protection in an experimental murine model against T. crassiceps cysticercosis when orally administered. This result supports the potential of HP6/TSOL18-carrot as a low-cost anti-cysticercosis vaccine candidate.

Cysticercosis is a zoonosis caused by Taenia solium that can be prevented by interrupting the parasite life cycle through pig vaccination. Several injectable vaccine candidates have been reported, but the logistic difficulties and costs for its application limited its use in nationwide control programs. Oral plant-based vaccines can deal with this limitation, because of their easy administration and low cost. A stable expression of the HP6/TSOL18 anti-T. solium cysticercosis protective antigen in carrot calli transformed with an optimized transgene is herein reported. An antigen accumulation up to 14 µg g−1 of dry-weight biomass was achieved in the generated carrot lines. Mouse immunization with one of the transformed calli induced both specific IgG and IgA anti-HP6/TSOL18 antibodies. A statistically significant reduction in the expected number of T. crassiceps cysticerci was observed in mice orally immunized with carrot-made HP6/TSOL18, in a similar extent to that obtained by subcutaneous immunization with recombinant HP6/TSOL18 protein. In this study, a new oral plant-made version of the HP6/TSOL18 anti-cysticercosis vaccine is reported. The vaccine candidate should be further tested against porcine cysticercosis.


Cysticercosis Delivery system HP6/TSOL18 Oral vaccine Plant-based vaccine Taenia Vaccination 



Enzyme replacement therapy


Glutathione S-transferase-HP6/TSOL18 fusion protein




Central nervous system



The authors acknowledge Georgina Diaz and Daniel Garzón for their technical support, and Juan Francisco Rodriguez for the language edition of this manuscript. This research was partially supported by the CONACyT (201448, 152793), DGAPA (IG-200414), and The Programa de Investigación para el Desarrollo y la Optimización de Vacunas, Adyuvantes y Métodos Diagnósticos del Instituto de Investigaciones Biomédicas, UNAM.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Elizabeth Monreal-Escalante
    • 1
  • Dania O. Govea-Alonso
    • 1
  • Marisela Hernández
    • 2
  • Jacquelynne Cervantes
    • 2
  • Jorge A. Salazar-González
    • 1
  • Andrea Romero-Maldonado
    • 1
  • Gabriela Rosas
    • 3
  • Teresa Garate
    • 4
  • Gladis Fragoso
    • 2
  • Edda Sciutto
    • 2
  • Sergio Rosales-Mendoza
    • 1
  1. 1.Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias QuímicasUniversidad Autónoma de San Luis PotosíSan Luis PotosíMexico
  2. 2.Dpto. Inmunología. Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de México, Circuito Escolar, Ciudad UniversitariaMexicoMexico
  3. 3.Facultad de MedicinaUniversidad Autónoma del Estado de MorelosCuernavacaMexico
  4. 4.Dpto. de Parasitología, Centro Nacional de MicrobiologíaInstituto de Salud Carlos IIIMadridSpain

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