Planta

, Volume 243, Issue 2, pp 337–354 | Cite as

Immunolocalization of cell wall carbohydrate epitopes in seaweeds: presence of land plant epitopes in Fucus vesiculosus L. (Phaeophyceae)

  • Sandra Cristina Raimundo
  • Utku Avci
  • Christina Hopper
  • Sivakumar Pattathil
  • Michael G. Hahn
  • Zoë A. Popper
Original Article
First Online:
Received:
Accepted:

Abstract

Main conclusion

Land plant cell wall glycan epitopes are present in Fucus vesiculosus. RG-I/AG mAbs recognize distinct glycan epitopes in structurally different galactans, and 3-linked glucans are also present in the cell walls.

Cell wall-directed monoclonal antibodies (mAbs) have given increased knowledge of fundamental land plant processes but are not extensively used to study seaweeds. We profiled the brown seaweed Fucus vesiculosus glycome employing 155 mAbs that recognize predominantly vascular plant cell wall glycan components. The resulting profile was used to inform in situ labeling studies. Several of the mAbs recognized and bound to epitopes present in different thallus parts of Fucus vesiculosus. Antibodies recognizing arabinogalactan epitopes were divided into four groups based on their immunolocalization patterns. Group 1 bound to the stipe, blade, and receptacles. Group 2 bound to the antheridia, oogonia and paraphyses. Group 3 recognized antheridia cell walls and Group 4 localized on the antheridia inner wall and oogonia mesochite. This study reveals that epitopes present in vascular plant cell walls are also present in brown seaweeds. Furthermore, the diverse in situ localization patterns of the RG-I/AG clade mAbs suggest that these mAbs likely detect distinct epitopes present in structurally different galactans. In addition, 3-linked glucans were also detected throughout the cell walls of the algal tissues, using the β-glucan-directed LAMP mAb. Our results give insights into cell wall evolution, and diversify the available tools for the study of brown seaweed cell walls.

Keywords

Brown seaweed Cell wall Galactans Glycome profiling Monoclonal antibodies 3-Linked glucan 

Abbreviations

AG-3

Arabinogalactan-3

AGP

Arabinogalactan protein

AIR

Alcohol insoluble residue

Ara

Arabinose

ELISA

Enzyme-linked immunosorbent assay

FCSPs

Fucose-containing sulphated polysaccharides

Fuc

Fucose

Gal

Galactose

GC/MS

Gas chromatography/mass spectrometry

Glc

Glucose

GulA

Guluronic acid

KPBS

Potassium phosphate buffered saline

mAb

Monoclonal antibody

Man

Mannose

ManA

Mannuronic acid

RG-I

Rhamnogalacturonan I

RG-I/AG

Rhamnogalacturonan I/arabinogalactan

TMB

3,3′,5,5′-Tetramethylbenzidine

TMS

Per-O-trimethylsilyl

Xyl

Xylose

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Notes

Acknowledgments

The research presented in this manuscript is funded as part of Science Foundation Ireland (SFI) Research Frontiers Programme (grant 11/RFP/EOB/3345 awarded to Z.A.P). The generation of the CCRC series of mAbs used in this work was supported by the US National Science Foundation Plant Genome Program (Awards DBI-0421683 and IOB-0923992 to M.G.H.). The monosaccharide composition analysis was supported by the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, US Department of Energy (grant DE-FG02-93ER20097 to Parastoo Azadi) at the Complex Carbohydrate Research Center. The authors thank Dr Udo Nitschke (Botany and Plant Science, School of Natural Sciences, and Ryan Institute, NUI Galway) for helpful comments during the preparation of the manuscript.

Supplementary material

425_2015_2412_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 22 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sandra Cristina Raimundo
    • 1
    • 2
  • Utku Avci
    • 2
  • Christina Hopper
    • 2
  • Sivakumar Pattathil
    • 2
  • Michael G. Hahn
    • 2
  • Zoë A. Popper
    • 1
  1. 1.Botany and Plant Science and Ryan Institute for Environmental, Marine and Energy Research, School of Natural SciencesNational University of Ireland GalwayGalwayIreland
  2. 2.Complex Carbohydrate Research CenterUniversity of GeorgiaAthensUSA

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