, Volume 243, Issue 1, pp 217–229 | Cite as

Silicon fertilization of potato: expression of putative transporters and tuber skin quality

  • Vijaya K. R. Vulavala
  • Rivka Elbaum
  • Uri Yermiyahu
  • Edna Fogelman
  • Akhilesh Kumar
  • Idit Ginzberg
Original Article


Main conclusion

A silicon transporter homolog was upregulated by Si fertilization and drought in potato roots and leaves. High Si in tuber skin resulted in anatomical and compositional changes suggesting delayed skin maturation.

Silicon (Si) fertilization has beneficial effects on plant resistance to biotic and abiotic stresses. Potatoes, low Si accumulators, are susceptible to yield loss due to suboptimal growth conditions; thus Si fertilization may contribute to crop improvement. The effect of Si fertilization on transcript levels of putative transporters, Si uptake and tuber quality was studied in potatoes grown in a glasshouse and fertilized with sodium silicate, under normal and drought-stress conditions. Anatomical studies and Raman spectroscopic analyses of tuber skin were conducted. A putative transporter, StLsi1, with conserved amino acid domains for Si transport, was isolated. The StLsi1 transcript was detected in roots and leaves and its level increased twofold following Si fertilization, and about fivefold in leaves upon Si × drought interaction. Nevertheless, increased Si accumulation was detected only in tuber peel of Si-fertilized plants—probably due to passive movement of Si from the soil solution—where it modified skin cell morphology and cell-wall composition. Compared to controls, skin cell area was greater, suberin biosynthetic genes were upregulated and skin cell walls were enriched with oxidized aromatic moieties suggesting enhanced lignification and suberization. The accumulating data suggest delayed tuber skin maturation following Si fertilization. Despite StLsi1 upregulation, low accumulation of Si in roots and leaves may result from low transport activity. Study of Si metabolism in potato, a major staple food, would contribute to the improvement of other low Si crops to ensure food security under changing climate.


Potato (Solanum tuberosum L.) Potato skin Raman spectroscopy Silicon fertilization Silicon transporter Suberization Tuber periderm 



The authors would like to thank Zechariah Tanami from the Volcani Center for technical assistance. The research was funded by the Chief Scientist at the Ministry of Agriculture, and is a contribution of ARO, the Volcani Center.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

425_2015_2401_MOESM1_ESM.pdf (173 kb)
Supplementary material 1 (PDF 172 kb)


  1. Agarwal U (2014) 1064 nm FT-Raman spectroscopy for investigations of plant cell walls and other biomass materials. Front Plant Sci 5:490PubMedPubMedCentralCrossRefGoogle Scholar
  2. Barel G, Ginzberg I (2008) Potato skin proteome is enriched with plant defence components. J Exp Bot 59:3347–3357PubMedPubMedCentralCrossRefGoogle Scholar
  3. Bernards MA (2002) Demystifying suberin. Can J Bot 80:227–240CrossRefGoogle Scholar
  4. Britez RM, Watanabe T, Jansen S, Reissmann CB, Osaki M (2002) The relationship between aluminium and silicon accumulation in leaves of Faramea marginata (Rubiaceae). New Phytol 156:437–444CrossRefGoogle Scholar
  5. Cai K, Gao D, Luo S, Zeng R, Yang J, Zhu X (2008) Physiological and cytological mechanisms of silicon-induced resistance in rice against blast disease. Physiol Plant 134:324–333PubMedCrossRefGoogle Scholar
  6. Cao A, Liquier J, Taillandier E (1995) Infrared and Raman spectroscopy of biomolecules. In: Schrader B (ed) Infrared and Raman spectroscopy. Methods and applications, Wiley, pp 344–371Google Scholar
  7. Cooke J, Leishman MR (2011) Is plant ecology more siliceous than we realise? Trends Plant Sci 16:61–68PubMedCrossRefGoogle Scholar
  8. Crusciol CAC, Pulz AL, Lemos LB, Soratto RP, Lima GPP (2009) Effects of silicon and drought stress on tuber yield and leaf biochemical characteristics in potato. Crop Sci 49:949–954CrossRefGoogle Scholar
  9. Currie HA, Perry CC (2009) Chemical evidence for intrinsic ‘Si’ within Equisetum cell walls. Phytochemistry 70:2089–2095PubMedCrossRefGoogle Scholar
  10. Deshmukh RK, Vivancos J, Ramakrishnan G, Guérin V, Carpentier G, Sonah H, Labbé C, Isenring P, Belzile F, Bélanger RR (2015) A precise spacing between NPA domains of aquaporins is essential for silicon permeability in plants. Plant J 83:489–500PubMedCrossRefGoogle Scholar
  11. Edwards HGM, Falk MJP (1997) Fourier-transform Raman spectroscopic study of unsaturated and saturated waxes. Spectrochim Acta A 53:2685–2694CrossRefGoogle Scholar
  12. Epstein E (1994) The anomaly of silicon in plant biology. P Natl Acad Sci USA 91:11–17CrossRefGoogle Scholar
  13. Epstein E (1999) Silicon. Annu Rev Plant Phys 50:641–664CrossRefGoogle Scholar
  14. Fauteux F, Chain F, Belzile F, Menzies JG, Belanger RR (2006) The protective role of silicon in the Arabidopsis-powdery mildew pathosystem. P Natl Acad Sci USA 103:17554–17559CrossRefGoogle Scholar
  15. Fleck AT, Nye T, Repenning C, Stahl F, Zahn M, Schenk MK (2011) Silicon enhances suberization and lignification in roots of rice (Oryza sativa). J Exp Bot 62:2001–2011PubMedPubMedCentralCrossRefGoogle Scholar
  16. Forrest K, Bhave M (2007) Major intrinsic proteins (MIPs) in plants: a complex gene family with major impacts on plant phenotype. Funct Integr Genomics 7:263–289PubMedCrossRefGoogle Scholar
  17. Fu D, Libson A, Miercke LJW, Weitzman C, Nollert P, Krucinski J, Stroud RM (2000) Structure of a glycerol-conducting channel and the basis for its selectivity. Science 290:481–486PubMedCrossRefGoogle Scholar
  18. Ghareeb H, Bozso Z, Ott PG, Repenning C, Stahl F, Wydra K (2011) Transcriptome of silicon-induced resistance against Ralstonia solanacearum in the silicon non-accumulator tomato implicates priming effect. Physiol Mol Plant 75:83–89CrossRefGoogle Scholar
  19. Gierlinger N (2014) Revealing changes in molecular composition of plant cell walls on the micron-level by Raman mapping and vertex component analysis (VCA). Front Plant Sci 5:306PubMedPubMedCentralCrossRefGoogle Scholar
  20. Gierlinger N, Schwanninger M (2006) Chemical imaging of poplar wood cell walls by confocal Raman microscopy. Plant Physiol 140:1246–1254PubMedPubMedCentralCrossRefGoogle Scholar
  21. Ginzberg I, Gerchikov N, Ziv E, Fogelman E, Warshavsky S, Tanami Z (2005) Potato tuber skin development: the effect of hot climate and plant desiccation. Acta Hort 684:93–98CrossRefGoogle Scholar
  22. Ginzberg I, Barel G, Ophir R, Tzin E, Tanami Z, Muddarangappa T, de Jong W, Fogelman E (2009) Transcriptomic profiling of heat-stress response in potato periderm. J Exp Bot 60:4411–4421PubMedCrossRefGoogle Scholar
  23. Ginzberg I, Minz D, Faingold I, Soriano S, Mints M, Fogelman E, Warshavsky S, Zig U, Yermiyahu U (2012) Calcium mitigated potato skin physiological disorder. Am J Potato Res 89:351–362CrossRefGoogle Scholar
  24. Hänninen T, Kontturi E, Vuorinen T (2011) Distribution of lignin and its coniferyl alcohol and coniferyl aldehyde groups in Picea abies and Pinus sylvestris as observed by Raman imaging. Phytochemistry 72:1889–1895PubMedCrossRefGoogle Scholar
  25. Haynes KG, Gergela DM, Hutchinson CM, Yencho GC, Clough ME, Henninger MR, Halseth DE, Sandsted E, Porter GA, Ocaya PC (2012) Early generation selection at multiple locations may identify potato parents that produce more widely adapted progeny. Euphytica 186:573–583CrossRefGoogle Scholar
  26. He C, Wang L, Liu J, Liu X, Li X, Ma J, Lin Y, Xu F (2013) Evidence for ‘silicon’ within the cell walls of suspension-cultured rice cells. New Phytol 200:700–709PubMedCrossRefGoogle Scholar
  27. Hodson MJ, Evans DE (1995) Aluminium/silicon interactions in higher plants. J Exp Bot 46:161–171CrossRefGoogle Scholar
  28. Hove R, Bhave M (2011) Plant aquaporins with non-aqua functions: deciphering the signature sequences. Plant Mol Biol 75:413–430PubMedCrossRefGoogle Scholar
  29. Iwama K (2008) Physiology of the potato: new insights into root system and repercussions for crop management. Potato Res 51:333–353CrossRefGoogle Scholar
  30. Katz O (2014) Beyond grasses: the potential benefits of studying silicon accumulation in non-grass species. Front Plant Sci 5:376PubMedPubMedCentralCrossRefGoogle Scholar
  31. Khandekar S, Leisner S (2011) Soluble silicon modulates expression of Arabidopsis thaliana genes involved in copper stress. J Plant Physiol 168:699–705PubMedCrossRefGoogle Scholar
  32. Kim SG, Kim KW, Park EW, Choi D (2002) Silicon-induced cell wall fortification of rice leaves: a possible cellular mechanism of enhanced host resistance to blast. Phytopathology 92:1095–1103PubMedCrossRefGoogle Scholar
  33. Kolattukudy PE (1977) Lipid polymers and associated phenols, their chemistry, biosynthesis and role in pathogenesis. Recent Adv Phytochem 77:185–246Google Scholar
  34. Krits P, Fogelman E, Ginzberg I (2007) Potato steroidal glycoalkaloid levels and the expression of key isoprenoid metabolic genes. Planta 227:143–150PubMedCrossRefGoogle Scholar
  35. Levin IW, Lewis EN (1990) Fourier transform Raman spectroscopy of biological materials. Anal Chem 62:1101A–1111APubMedGoogle Scholar
  36. Levy D (1985) The response of potatoes to a single transient heat or drought stress imposed at different stages of tuber growth. Potato Res 28:415–424CrossRefGoogle Scholar
  37. Levy D (1992) The response of potatoes (Solanum tuberosum L.) to salinity: plant growth and tuber yields in the arid desert of Israel. Ann Appl Biol 120:547–555CrossRefGoogle Scholar
  38. Levy D, Veilleux RE (2007) Adaptation of potato to high temperatures and salinity-a review. Am J Potato Res 84:487–506CrossRefGoogle Scholar
  39. Levy D, Fogelman E, Itzhak Y (1988) The effect of water salinity on potatoes (Solanum tuberosum L.): physiological indices and yielding capacity. Potato Res 31:601–610CrossRefGoogle Scholar
  40. Levy D, Coleman WK, Veilleux RE (2013) Adaptation of potato to water shortage: irrigation management and enhancement of tolerance to drought and salinity. Am J Potato Res 90:186–206CrossRefGoogle Scholar
  41. Lux A, Luxova M, Hattori T, Inanaga S, Sugimoto Y (2002) Silicification in sorghum (Sorghum bicolor) cultivars with different drought tolerance. Physiol Plant 115:87–92PubMedCrossRefGoogle Scholar
  42. Ma JF (2004) Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. J Soil Sci Plant Nut 50:11–18CrossRefGoogle Scholar
  43. Ma JF, Yamaji N (2006) Silicon uptake and accumulation in higher plants. Trends Plant Sci 11:392–397PubMedCrossRefGoogle Scholar
  44. Ma JF, Tamai K, Yamaji N, Mitani N, Konishi S, Katsuhara M, Ishiguro M, Murata Y, Yano M (2006) A silicon transporter in rice. Nature 440:688–691PubMedCrossRefGoogle Scholar
  45. Ma JF, Yamaji N, Mitani N, Tamai K, Konishi S, Fujiwara T, Katsuhara M, Yano M (2007) An efflux transporter of silicon in rice. Nature 448:209–212PubMedCrossRefGoogle Scholar
  46. Mitani N, Ma JF (2005) Uptake system of silicon in different plant species. J Exp Bot 56:1255–1261PubMedCrossRefGoogle Scholar
  47. Mitani N, Chiba Y, Yamaji N, Ma JF (2009) Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice. Plant Cell 21:2133–2142PubMedPubMedCentralCrossRefGoogle Scholar
  48. Mitani N, Yamaji N, Ago Y, Iwasaki K, Ma JF (2011) Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation. Plant J 66:231–240PubMedCrossRefGoogle Scholar
  49. Mitani-Ueno N, Yamaji N, Ma JF (2011a) Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake. Plant Signal Behav 6:991–994PubMedPubMedCentralCrossRefGoogle Scholar
  50. Mitani-Ueno N, Yamaji N, Zhao FJ, Ma JF (2011b) The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic. J Exp Bot 62:4391–4398PubMedPubMedCentralCrossRefGoogle Scholar
  51. Peleg Z, Saranga Y, Fahima T, Aharoni A, Elbaum R (2010) Genetic control over silica deposition in wheat awns. Physiol Plant 140:10–20PubMedCrossRefGoogle Scholar
  52. Pilon C, Soratto RP, Moreno LA (2013) Effects of soil and foliar application of soluble silicon on mineral nutrition, gas exchange, and growth of potato plants. Crop Sci 53:1605–1614CrossRefGoogle Scholar
  53. Prinsloo LC, du Plooy W, van der Merwe C (2004) Raman spectroscopic study of the epicuticular wax layer of mature mango (Mangifera indica) fruit. J Raman Spectrosc 35:561–567CrossRefGoogle Scholar
  54. Ranathunge K, Schreiber L, Franke R (2011) Suberin research in the genomics era-new interest for an old polymer. Plant Sci 180:399–812PubMedCrossRefGoogle Scholar
  55. Reeve RM, Hautala E, Weaver ML (1969) Anatomy and compositional variation within potatoes 1. Developmental histology of the tuber. Am Potato J 46:361–373CrossRefGoogle Scholar
  56. Romero-Aranda MR, Jurado O, Cuartero J (2006) Silicon alleviates the deleterious salt effect on tomato plant growth by improving plant water status. J Plant Physiol 163:847–855PubMedCrossRefGoogle Scholar
  57. Ruzin SE (1999) Plant microtechnique and microscopy. Oxford University Press, New YorkGoogle Scholar
  58. Santos S, Graça J (2013) Stereochemistry of C18 monounsaturated cork suberin acids determined by spectroscopic techniques including 1H-NMR multiplet analysis of olefinic protons. Phytochem Anal 25:192–200PubMedCrossRefGoogle Scholar
  59. Schoelynck J, Bal K, Backx H, Okruszko T, Meire P, Struyf E (2010) Silica uptake in aquatic and wetland macrophytes: a strategic choice between silica, lignin and cellulose? New Phytol 186:385–391PubMedCrossRefGoogle Scholar
  60. Serra O, Soler M, Hohn C, Franke R, Schreiber L, Prat S, Molinas M, Figueras M (2009a) Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration. J Exp Bot 60:697–707PubMedPubMedCentralCrossRefGoogle Scholar
  61. Serra O, Soler M, Hohn C, Sauveplane V, Pinot F, Franke R, Schreiber L, Prat S, Molinas M, Figueras M (2009b) CYP86A33-targeted gene silencing in potato tuber alters suberin composition, distorts suberin lamellae, and impairs the periderm’s water barrier function. Plant Physiol 149:1050–1060PubMedPubMedCentralCrossRefGoogle Scholar
  62. Soler M, Serra O, Fluch S, Molinas M, Figueras M (2011) A potato skin SSH library yields new candidate genes for suberin biosynthesis and periderm formation. Planta 233:933–978PubMedCrossRefGoogle Scholar
  63. Sui H, Han B-G, Lee JK, Walian P, Jap BK (2001) Structural basis of water-specific transport through the AQP1 water channel. Nature 414:872–878PubMedCrossRefGoogle Scholar
  64. Susnoschi M (1982) Growth and yield studies of potatoes developed in a semi-arid region 1. Yield response of several varieties grown as a double crop. Potato Res 25:59–69CrossRefGoogle Scholar
  65. Vaculík M, Landberg T, Greger M, Luxová M, Stoláriková M, Lux A (2012) Silicon modifies root anatomy, and uptake and subcellular distribution of cadmium in young maize plants. Ann Bot 110:433–443PubMedPubMedCentralCrossRefGoogle Scholar
  66. Van Bockhaven J, De Vleesschauwer D, Hofte M (2013) Towards establishing broad-spectrum disease resistance in plants: silicon leads the way. J Exp Bot 64:1281–1293PubMedCrossRefGoogle Scholar
  67. Vishwanath S, Delude C, Domergue F, Rowland O (2014) Suberin: biosynthesis, regulation, and polymer assembly of a protective extracellular barrier. Plant Cell Rep. doi: 10.1007/s00299-014-1727-z PubMedGoogle Scholar
  68. Wishart J, George T, Brown L, Ramsay G, Bradshaw J, White P, Gregory P (2013) Measuring variation in potato roots in both field and glasshouse: the search for useful yield predictors and a simple screen for root traits. Plant Soil 368:231–249CrossRefGoogle Scholar
  69. Yamaji N, Mitatni N, Ma JF (2008) A transporter regulating silicon distribution in rice shoots. Plant Cell 20:1381–1389PubMedPubMedCentralCrossRefGoogle Scholar
  70. Yamanaka S, Takeda H, Komatsubara S, Ito F, Usami H, Togawa E, Yoshino K (2009) Structures and physiological functions of silica bodies in the epidermis of rice plants. Appl Phys Lett 95:123703CrossRefGoogle Scholar
  71. Zellner W, Frantz J, Leisner S (2011) Silicon delays Tobacco ringspot virus systemic symptoms in Nicotiana tabacum. J Plant Physiol 168:1866–1869PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Vijaya K. R. Vulavala
    • 1
    • 2
  • Rivka Elbaum
    • 2
  • Uri Yermiyahu
    • 3
  • Edna Fogelman
    • 1
  • Akhilesh Kumar
    • 1
  • Idit Ginzberg
    • 1
  1. 1.Institute of Plant SciencesAgricultural Research Organization, Volcani CenterBet DaganIsrael
  2. 2.The Robert H. Smith Institute of Plant Sciences and Genetics in AgricultureFaculty of Agriculture, Hebrew University of JerusalemRehovotIsrael
  3. 3.Institute of Soil and WaterAgricultural Research Organization, Gilat CenterNegevIsrael

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