Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria
- 562 Downloads
Profiling of plant secondary metabolite allows to differentiate the different types of ecological interactions established between rice and bacteria. Rice responds to ecologically distinct bacteria by altering its content of flavonoids and hydroxycinnamic acid derivatives.
Plants’ growth and physiology are strongly influenced by the biotic interactions that plants establish with soil bacterial populations. Plants are able to sense and to respond accordingly to ecologically distinct bacteria, by inducing defense pathways against pathogens to prevent parasitic interactions, and by stimulating the growth of root-associated beneficial or commensal bacteria through root exudation. Plant secondary metabolism is expected to play a major role in this control. However, secondary metabolite responses of a same plant to cooperative, commensal and deleterious bacteria have so far never been compared. The impact of the plant growth-promoting rhizobacteria (PGPR) Azospirillum lipoferum 4B on the secondary metabolite profiles of two Oryza sativa L. cultivars (Cigalon and Nipponbare) was compared to that of a rice pathogen Burkholderia glumae AU6208, the causing agent of bacterial panicle blight and of a commensal environmental bacteria Escherichia coli B6. Root and shoot rice extracts were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Principal component analyses (PCAs) pinpointed discriminant secondary metabolites, which were characterized by mass spectrometry. Direct comparison of metabolic profiles evidenced that each bacterial ecological interaction induced distinct qualitative and quantitative modifications of rice secondary metabolism, by altering the content of numerous flavonoid compounds and hydroxycinnamic acid (HCA) derivatives. Secondary metabolism varied according to the cultivars and the interaction types, demonstrating the relevance of secondary metabolic profiling for studying plant–bacteria biotic interactions.
KeywordsCommensalism Cooperation Flavonoids Hydroxycinnamic acid Parasitism Rice secondary metabolite profiling
Principal component analysis
Plant growth-promoting rhizobacteria
We are grateful to Floriant Bellvert, Guillaume Meiffren, Benoît Drogue, Hervé Sanguin and Marjolaine Rey for technical assistance and helpful discussion (UMR CNRS 5557 Ecologie Microbienne). We wish to thank V. Venturi (ICGEB, Trieste, Italy) for gift of the B. glumae AU6208 strain. We are grateful to C. Louvel (Centre français du riz, Arles, France) and J.B. Morel (BGPI, Montpellier, France) for gift of Cigalon and Nipponbare seeds, respectively. This work was supported by a fellowship from the “Centre National de la Recherche Scientifique” to A.C. and by the ANR project AZORIZ (ANR-08-BLAN-0098). This work made use of the technical platforms “Serre” at FR 41 (Université Lyon 1) and “Centre d’Etude des Substances Naturelles” (UMR CNRS 5557 Ecologie Microbienne).
- Camilios-Neto D, Bonato P, Wassem R, Tadra-Sfeir MZ, Brusamarello-Santos LCC, Valdameri G, Donatti L, Faoro H, Weiss VA, Chubatsu LS, Pedrosa FO, Souza EM (2014) Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes. BMC Genomics 15:378PubMedCentralCrossRefPubMedGoogle Scholar
- Couillerot O, Ramírez-Trujillo A, Walker V, von Felten A, Jansa J, Maurhofer M, Défago G, Prigent-Combaret C, Comte G, Caballero-Mellado J, Moënne-Loccoz Y (2013) Comparison of prominent Azospirillum strains in Azospirillum–Pseudomonas–Glomus consortia for promotion of maize growth. Appl Microbiol Biotechnol 97:4639–4649CrossRefPubMedGoogle Scholar
- Edreva AV, Velikova T, Tsonev T, Dagnon S, Gurel A, Atkas L, Gesheva E (2008) Stress protective role of secondary metabolites: diversity of functions and mechanisms. Gen Appl Plant Physiol 34:67–78Google Scholar
- Mubassara S, Zahed UM, Motiur RM, Patwary FK, Akond MA (2008) Seed inoculation effect of Azospirillum spp. on growth, biomass and yield parameter of wheat. Acad J Plant Sci 1:56–61Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Walker V, Couillerot O, Von Felten A, Bellvert F, Jansa J, Maurhofer M, Bally R, Moënne-Loccoz Y, Comte G (2012) Variation of secondary metabolite levels in maize seedling roots induced by inoculation with Azospirillum, Pseudomonas and Glomus consortium under field conditions. Plant Soil 356:151–163CrossRefGoogle Scholar