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Planta

, Volume 242, Issue 5, pp 1207–1219 | Cite as

Water loss from litchi (Litchi chinensis) and longan (Dimocarpus longan) fruits is biphasic and controlled by a complex pericarpal transpiration barrier

  • Markus RiedererEmail author
  • Katja Arand
  • Markus Burghardt
  • Hua Huang
  • Michael Riedel
  • Ann-Christin Schuster
  • Anna Smirnova
  • Yueming Jiang
Original Article

Abstract

Main conclusion

In litchi and longan fruits, a specialised pericarp controls water loss by a protective system consisting of two resistances in series and two water reservoirs separated by a barrier.

In the fruits of litchi (Litchi chinensis) and longan (Dimocarpus longan), the pericarp is solely a protective structure lacking functional stomata and completely enclosing the aril that is the edible part. Maintaining a high water content of the fruits is crucial for ensuring the economic value of these important fruit crops. The water loss rates from mature fruits were determined and analysed in terms of the properties of the pericarps. Water loss kinetics and sorption isotherms were measured gravimetrically. The pericarps were studied with microscopy, and cuticular waxes and cutin were analysed with gas chromatography and mass spectrometry. The kinetics of fruit water loss are biphasic with a high initial rate and a lower equilibrium rate lasting for many hours. The outer and inner surfaces of the pericarps are covered with cuticles. Litchi and longan fruits have a unique type of transpiration barrier consisting of two resistances in series (endo- and exocarp cuticles) and two reservoirs of water (aril and mesocarp). The exocarp permeability controls the water loss from fresh fruits while in fruits kept for an extended time at low relative humidity it is determined by the endo- and exocarp permeabilities. Permeances measured are within the range for typical fruit cuticles. The findings may be used to design optimal postharvest storage strategies for litchi and longan fruits.

Keywords

Cuticular waxes Pericarp Plant cuticle Water loss kinetics Water permeability Water sorption 

Abbreviations

P

Permeance

RH

Relative humidity

WLR

Water loss rate

Notes

Acknowledgments

This work was supported by a Chinese Academy of Sciences Visiting Professorship for Senior International Scientists grant no. 2011T2S31 to M. R., and the Overseas Study Program of Guangzhou Elite Project Scholarship to H. H. The authors also gratefully acknowledge the contributions of Franz Olbrich, Frankfurt, and the skilful technical assistance by Natascha Sieling. The authors are also indebted to two anonymous reviewers for valuable comments that helped to considerably improve this manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

425_2015_2360_MOESM1_ESM.docx (28 kb)
Supplementary material 1 (DOCX 28 kb)

References

  1. Al-Muhtaseb AH, McMinn WAM, Magee TRA (2002) Moisture sorption isotherm characteristics of food products: a review. Food Bioprod Process 80:118–128CrossRefGoogle Scholar
  2. Baker EA (1982) Chemistry and morphology of plant epicuticular waxes. In: Cutler DF, Alvin KL, Price CE (eds) The plant cuticle. Academic Press, London, pp 139–165Google Scholar
  3. Bell LN, Labuza TP (2000) Moisture sorption: practical aspects of isotherm measurement and use. American Association for Cereal Chemists, St. PaulGoogle Scholar
  4. Bryant PH (2012) A model of postharvest moisture loss under air currents to reduce pericarp browning of litchi (Litchi chinensis Sonn.). Postharvest Biol Technol 73:8–13CrossRefGoogle Scholar
  5. Buda GJ, Isaacson T, Matas AJ, Paolillo DJ, Rose JKC (2009) Three-dimensional imaging of plant cuticle architecture using confocal scanning laser microscopy. Plant J 60:378–385CrossRefPubMedGoogle Scholar
  6. Burghardt M, Riederer M (2006) Cuticular transpiration. In: Riederer M, Müller C (eds) Biology of the plant cuticle. Blackwell Publishing, Oxford, pp 291–310Google Scholar
  7. Buschmann C, Langsdorf G, Lichtenthaler HK (2000) Imaging of the blue, green, and red fluorescence emission of plants: an overview. Photosynthetica 38:483–491CrossRefGoogle Scholar
  8. Cheke AS, Dahl JF (1981) The status of bats on western Indian Ocean islands, with special reference to Pteropus. Mammalia 45:205–238CrossRefGoogle Scholar
  9. Fernandez S, Osorio S, Heredia A (1999) Monitoring and visualising plant cuticles by confocal laser scanning microscopy. Plant Physiol Biochem 37:789–794CrossRefGoogle Scholar
  10. Flora of China Editorial Committee (2007a) Dimocarpus Loureiro,Fl.Cochinch.1: 233.1790. Flora of China, vol 12. Missouri Botanical Garden Press, St. Louis, pp 15–16Google Scholar
  11. Flora of China Editorial Committee (2007b) Litchi Sonnerat, Voy. Indes Orient. 3: 255.1782. Flora of China, vol 12. Missouri Botanical Garden Press, St. Louis, pp 16–17Google Scholar
  12. Forney CF, Brandl DG (1992) Control of humidity in small controlled-environment chambers using glycerol-water solutions. Horttechnology 2:52–54Google Scholar
  13. Holcroft DM, Mitcham EJ (1996) Postharvest physiology and handling of litchi (Litchi chinensis Sonn). Postharvest Biol Technol 9:265–281CrossRefGoogle Scholar
  14. Holloway PJ (1982) The chemical constitution of plant cutins. In: Cutler DF, Alvin KL, Price CE (eds) The plant cuticle. Academic Press, London, pp 45–85Google Scholar
  15. Holloway PJ (1984a) Cutins and suberins, the polymeric plant lipids. In: Mangold HK, Zweig G, Sherma J (eds) CRC handbook of chromatography. Lipids, vol 1. CRC Press, Boca Raton, pp 321–345Google Scholar
  16. Holloway PJ (1984b) Surface lipids of plants and animals. In: Mangold HK, Zweig G, Sherma J (eds) CRC handbook of chromatography. Lipids, vol 1. CRC Press, Boca Raton, pp 347–380Google Scholar
  17. Huahai Z, Changyou L, Zhiji G (1998) Experimental research on drying characteristics of litchi. Dry Technol 17:1915–1925CrossRefGoogle Scholar
  18. Huang H, Stern R (2013) Fruit set, development and maturation. In: Menzel CM, Waite G (eds) Litchi and longan. CABI Publishing, Wallingford, pp 115–14034Google Scholar
  19. Huang X, Subharabandhu S, Mitra SK, Ben-Arie R, Stern R (2013) Origin, history, production and processing. In: Menzel CM, Waite G (eds) Litchi and longan. CABI Publishing, Wallingford, pp 1–23Google Scholar
  20. Jeffree CE (2006) The fine structure of the plant cuticle. In: Riederer M, Müller C (eds) Biology of the plant cuticle, vol 23. Blackwell Publishing, Oxford, pp 11–125CrossRefGoogle Scholar
  21. Jetter R, Kunst L, Samuels AL (2006) Composition of plant cuticular waxes. In: Riederer M, Müller C (eds) Biology of the plant cuticle, vol 23. Blackwell Publishing, Oxford, pp 145–181CrossRefGoogle Scholar
  22. Jiang YM, Fu JR (1999) Postharvest browning of litchi fruit by water loss and its prevention by controlled atmosphere storage at high relative humidity. Food Sci Technol 32:278–283Google Scholar
  23. Jiang YM, Li Y (2001) Effects of chitosan coating on postharvest life and quality of longan fruit. Food Chem 73:139–143CrossRefGoogle Scholar
  24. Jiang YM, Zhang ZQ, Joyce DC, Ketsa S (2002) Postharvest biology and handling of longan fruit (Dimocarpus longan Lour.). Postharvest Biol Technol 26:241–252CrossRefGoogle Scholar
  25. Jiang YM, Duan XW, Joyce D, Zhang ZQ, Li JR (2004) Advances in understanding of enzymatic browning in harvested litchi fruit. Food Chem 88:443–446CrossRefGoogle Scholar
  26. Jiang YM, Wang Y, Song L, Liu H, Lichter A, Kerdchoechuen O, Joyce DC, Shi J (2006) Postharvest characteristics and handling of litchi fruit - an overview. Aust J Exp Agr 46:1541–1556CrossRefGoogle Scholar
  27. Kerstiens G (2006) Water transport in plant cuticles: an update. J Exp Bot 57:2493–2499CrossRefPubMedGoogle Scholar
  28. Kissinger M, Tuvia-Alkalai S, Shalom Y, Fallik E, Elkind Y, Jenks MA, Goodwin MS (2005) Characterization of physiological and biochemical factors associated with postharvest water loss in ripe pepper fruit during storage. J Am Soc Hortic Sci 130:735–741Google Scholar
  29. Kuhn GD (1962) Dehydration studies of lychee fruit. Proc Fl State Hortic Soc 75:273–277Google Scholar
  30. Lara I, Belge B, Goulao LF (2013) The fruit cuticle as a modulator of postharvest quality. Postharvest Biol Technol 87:103–112CrossRefGoogle Scholar
  31. Leide J, Hildebrandt U, Reussing K, Riederer M, Vogg G (2007) The developmental pattern of tomato fruit wax accumulation and its impact on cuticular transpiration barrier properties: effects of a deficiency in a β-ketoacyl-coenzyme A synthase (LeCER6). Plant Physiol 144:1667–1679PubMedCentralCrossRefPubMedGoogle Scholar
  32. Li JG, Huang HB, Gao FF, Huang XM, Wang HC (2001) An overview of Litchi fruit cracking. Acta Hortic 558:205–208Google Scholar
  33. Lin HT, Chen L, Lin YF, Jiang YM (2013) Fruit weight loss and pericarp water loss of harvested longan fruit in relation to pericarp browning. In: Dongliang Q, Mitra SK, Diczbalis Y (eds) III International symposium on longan, lychee, and other fruit trees in Sapindaceae family. International Society for Horticultural Science, Korbeek-Lo, Belgium, pp 587–592Google Scholar
  34. Menzel CM, Waite G (2005) Litchi and longan—botany, production and uses. CABI Publishing, WallingfordCrossRefGoogle Scholar
  35. Nobel PS (2009) Physicochemical and environmental plant physiology. Academic Press, OxfordGoogle Scholar
  36. Parsons EP, Popopvsky S, Lohrey GT, Lu S, Alkalai-Tuvia S, Perzelan Y, Paran I, Fallik E, Jenks MA (2012) Fruit cuticle lipid composition and fruit post-harvest water loss in an advanced backcross generation of pepper (Capsicum sp.). Physiol Plant 146:15–25CrossRefPubMedGoogle Scholar
  37. Riederer M, Schreiber L (1995) Waxes—the transport barriers of plant cuticles. In: Hamilton RJ (ed) Waxes: chemistry, molecular biology and functions, vol 6. The Oily Press Library. The Oily Press, West Ferry, Dundee, Scotland, pp 130–156Google Scholar
  38. Riederer M, Friedmann A (2006) Transport of lipophilic non-electrolytes across the cuticle. In: Riederer M, Müller C (eds) Biology of the plant cuticle. Blackwell Publishing, Oxford, pp 249–278CrossRefGoogle Scholar
  39. Riederer M, Schreiber L (2001) Protecting against water loss: analysis of the barrier properties of plant cuticles. J Exp Bot 52:2023–2032CrossRefPubMedGoogle Scholar
  40. Riley RG, Kolattukudy PE (1975) Evidence for covalently attached p-coumaric acid and ferulic acid in cutins and suberins. Plant Physiol 56:650–654PubMedCentralCrossRefPubMedGoogle Scholar
  41. Sarni-Manchado P, Le Roux E, Le Guerneve C, Lozano Y, Cheynier V (2000) Phenolic composition of litchi fruit pericarp. J Agric Food Chem 48:5995–6002CrossRefPubMedGoogle Scholar
  42. Schreiber L (2010) Transport barriers made of cutin, suberin and associated waxes. Trends Plant Sci 15:546–553CrossRefPubMedGoogle Scholar
  43. Sivakumar D, Korsten L (2011) Litchi (Litchi chinensis Sonn). In: Yahia EM (ed) Postharvest biology and technology of tropical and subtropical fruits: cocona to mango, vol 3. Woodhead Publishing Limited, Oxford, Cambridge, Philadelphia, New Delhi, pp 361–407Google Scholar
  44. Stark RE, Tian SY (2006) The cutin biopolymer matrix. In: Riederer M, Müller C (eds) Biology of the plant cuticle, vol 23. Blackwell Publishing, Oxford, pp 126–144Google Scholar
  45. Subharabandhu S, Stern R (2005) Taxonomy, botany and plant development. In: Menzel CM, Waite G (eds) Litchi and longan. CABI Publishing, Wallingford, pp 25–34Google Scholar
  46. Tillman EA, Van Doom A, Avery ML (2000) Bird damage to tropical fruit in south Florida. Wildlife Damage Management Conferences–Proceedings, pp 1–14Google Scholar
  47. Tindall HD (1994) Sapindaceous fruits: botany and horticulture. In: Janick J (ed) Horticultural reviews, vol 16. Wiley, Oxford, pp 143–196Google Scholar
  48. Underhill SJR, Simons DH (1993) Lychee (Litchi chinensis Sonn.) pericarp desiccation and the importance of postharvest microcracking. Sci Hortic 54:287–294CrossRefGoogle Scholar
  49. van der Pijl L (1957) On the arilloids of Nephelium, Euphoria, Litchi and Aesculus, and the seeds of Sapindaceae in general. Acta Bot Neer 6:618–641CrossRefGoogle Scholar
  50. van der Wel GK, Adan OCG (1999) Moisture in organic coatings - a review. Prog Org Coat 37:1–14CrossRefGoogle Scholar
  51. Vogg G, Fischer S, Leide J, Emmanuel E, Jetter R, Levy AA, Riederer M (2004) Tomato fruit cuticular waxes and their effects on transpiration barrier properties: functional characterization of a mutant deficient in a very-long-chain fatty acid ß-ketoacyl-CoA synthase. J Exp Bot 55:1401–1410CrossRefPubMedGoogle Scholar
  52. Weidman Groff G (1921) The lychee and lungan. New York, London, Canton (China)Google Scholar
  53. Weidman Groff G (1943) Some ecological factors involved in successful lychee culture. Proc Fl State Hortic Soc 56:134–155Google Scholar
  54. Yeats TH, Rose JK (2013) The formation and function of plant cuticles. Plant Physiol 163:5–20PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Markus Riederer
    • 1
    Email author
  • Katja Arand
    • 1
  • Markus Burghardt
    • 1
  • Hua Huang
    • 1
    • 2
  • Michael Riedel
    • 1
  • Ann-Christin Schuster
    • 1
  • Anna Smirnova
    • 1
    • 3
  • Yueming Jiang
    • 2
  1. 1.Julius von Sachs Institute of Biological SciencesUniversity of WürzburgWürzburgGermany
  2. 2.South China Botanical Garden, Chinese Academy of SciencesGuangzhouPeople’s Republic of China
  3. 3.Génétique Moléculaire, Génomique, Microbiologie, Institut de Physiologie et de la Chimie BiologiqueUniversité de StrasbourgStrasbourg CedexFrance

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